Antioxidant activities of aqueous extracts from three cultivars of guava leaf

Jeong, Chang Ho; Bae, Young Il; Park, Soo Jung; Lee, Si Kyung; Hur, Sun

doi:10.1007/s10068-012-0207-x

Cited by 14 publications

(6 citation statements)

References 25 publications

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“…Another study found that 50% hydroethanolic extract of Salvia officinalis had the highest TPC (Khiya et al, 2021). TPC value of GL is close to 257.38 to 290.466 mg/g as described by Jeong et al (2012). Our values also agree with Morais-Braga et al ( 2017) findings (288.56 mg GAE/g dry extract) for 50% ethanol solvent.…”

Section: Phenolic and Flavonoid Contentsupporting

confidence: 90%

Phytochemical screening and in vitro radical scavenging activities of “Gola” guava fruit and leaf extracts

Shahid

Inam‐Ur‐Raheem

Aadil

et al. 2022

Food Processing Preservation

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This study aimed to investigate the chemical and antioxidant characteristics of 50% and 70% hydroethanolic Gola guava fruit (GF) and guava leaf (GL) extracts by high‐performance liquid chromatography (HPLC) and Fourier‐transform infrared spectroscopy (FTIR). Total phenolic content (TPC) was determined by Folin–Ciocalteu reagent. The ability to scavenge free radicals was evaluated using DPPH (2,2‐diphenyl‐1‐picrylhydrazyl), H2O2 (hydrogen peroxide), and ABTS+ (2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid)) in vitro assay. Fours hydroethanolic Gola GF and GL extracts (GF50%, GF70%, GL50%, and GL70%): 50% hydroethanolic leaves extract showed the highest TPC (299.06 ± 2.69 mg GAE/g dry extract) and TFC (31.05 ± 1.67 mg CE/g). GL50% (299.06 ± 2.69 mg GAE/g dry extract) showed high TPC than GL70%. While GL50% and GL70%’s antioxidant potential was higher than GF extracts. TPC showed a highly positive correlation with DPPH, H2O2, and a moderately positive correlation with ABTS·+. HPLC analysis indicated the presence of flavonoid compounds including quercetin and kaempferol as well as phenolic acids like gallic, syringic, m‐coumaric, p‐coumaric, vanillic, ferulic, benzoic, caffeic, chlorogenic, cinnamic, sinapic, and ascorbic acids. A high number of phenolic compounds was identified in GL50% followed by GF50% through HPLC. The extraction of bioactive compounds was more efficient with 50% hydroethanolic solvent. The FTIR spectrograms showed changes in the chemical groups and determined the extracts' functional groups. In conclusion, findings support the use of phenolic compounds extracted from GF and GL, not only for their antioxidant activity, but also provide an important basis for potential use of phenolic compounds as crosslinking agents of sulfated and non‐sulfated glycosaminoglycan. Novelty impact statement This study manifests the best extraction solvent for optimized recovery of all antioxidant compounds of Gola GF and GL at maximum potential and provides an insight into the chemical profile of extracts. The chemical characterization by HPLC and FTIR pave the way for the possible natural antioxidant's formulation and development of functional food with a specific investigation to elucidate the action of mechanism at the cellular and organism level to evaluate the pharmacological potential of extracts.

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Section: Phenolic and Flavonoid Contentsupporting

confidence: 90%

Phytochemical screening and in vitro radical scavenging activities of “Gola” guava fruit and leaf extracts

Shahid

Inam‐Ur‐Raheem

Aadil

et al. 2022

Food Processing Preservation

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“…In the last two decades, extensive efforts have focused on identifying and characterizing potential therapeutic plants as natural antioxidants sources to reduce the free radical‐ and peroxide‐induced cell and tissue injuries . Therefore, different procedures have been implemented to investigate the potential antioxidant activity of crude plant oils and extracts . In this study, we have employed the commonly used DPPH · and ABTS ·+ radical scavenging and metal ion chelating activity (MICA) assays to evaluate the antioxidant potentials of F‐PSEO and D‐PSEO.…”

Section: Resultsmentioning

confidence: 99%

Composition, Antioxidant, and Cytotoxic Activities of the Essential Oils from Fresh and Air‐Dried Aerial Parts of Pallenis spinosa

Al–Qudah

Saleh

Alhawsawi

et al. 2017

Chemistry & Biodiversity

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This study was performed to determine the chemical composition, antioxidant and cytotoxic effects of essential oils extracted from the aerial parts of fresh (F-PSEO) and air-dried (D-PSEO) Pallenis spinosa. The composition of the oils was analyzed by gas chromatography (GC) and GC/mass spectrometry, the antioxidant activity by free radical scavenging and metal chelating assays, and their cytotoxicity by a flow cytometry analysis. The primary components in both oils were sesquiterpene hydrocarbons and oxygentated sesquiterpenes. F-PSEO contained 36 different compounds; α-cadinol (16.48%), germacra-1(10),5-diene-3,4-diol (14.45%), γ-cadinene (12.03%), and α-muurolol (9.89%) were the principal components. D-PSEO contained 53 molecules; α-cadinol (19.26%), δ-cadinene (13.93%), α-muurolol (12.88%), and germacra-1(10),5-diene-3,4-diol (8.41%) constituted the highest percentages. Although both oils exhibited a weak radical scavenging and chelating activity, compared to α-tocopherol and ascorbic acid, D-PSEO showed a 2-fold greater antioxidant activity than F-PSEO. Furthermore, low doses of F-PSEO were able to inhibit the growth of leukemic (HL-60, K562, and Jurkat) and solid tumor cells (MCF-7, HepG2, HT-1080, and Caco-2) with an IC range of 0.25 - 0.66 μg/ml and 0.50 - 2.35 μg/ml, respectively. F-PSEO showed a ca. 2 - 3-fold stronger cytotoxicity against the tested cells than D-PSEO. The potent growth inhibitory effect of the plant essential oil encourages further studies to characterize the molecular mechanisms of its cytotoxicity.

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“…The main phenolic compounds reported in guava leaves extracts are gallic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, rutin, quercetin, kaempferol, and luteolin [ 20 ]. The phenolic compounds in guava leave extracts may vary depending on the variety, the way to dry the leaves, extraction technique, and the maturity of the leaves [ 16 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…Siwarungson et al [ 22 ] used three varieties of Psidium guajava grown in Thailand, measuring total phenols, DPPH, and hydroxyl radicals. Jeong et al [ 21 ] measured total phenolic compounds, ABTS, and DPPH in three varieties of Psidium guajava cultivated in Korea. These assays are used due to the versatility of the samples that can be analyzed from cell lines to nutrition lines, since it shows a premise of the structure and its relationship in the antioxidant activity of the compounds contained in the samples [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.

et al. 2018

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Guava leaf (Psidium guajava L.) extracts are used in both traditional medicine and the pharmaceutical industry. The antioxidant compounds in P. guajava leaves can have positive effects including anti-inflammatory, anti-hyperglycemic, hepatoprotective, analgesic, anti-cancer effects, as well as protecting against cardiovascular diseases. In the present study, phenolic compounds and in vitro antioxidant capacity were measured in extracts obtained with polar and non-polar solvents from leaves of two varieties of guava, Calvillo Siglo XXI and Hidrozac. The quantity of total phenolics and total flavonoids were expressed as equivalents of gallic acid and quercetin, respectively. Hydroxyl radical, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and Oxygen Radical Absorbance Capacity using fluorescein (ORAC-FL) in vitro tests were used to assess the radical scavenging abilities of the extracts. The total phenolics were higher in the aqueous fraction of the variety Calvillo Siglo XXI, while in the Hidrozac variety total phenolics were higher in the acetone and chloroform fractions. Total flavonoids were higher in all fractions in the variety Calvillo Siglo XXI. Total phenolics showed a highly positive correlation for ORAC-FL, and a moderately positive correlation with hydroxyl radicals. Finally, total flavonoids showed a slightly positive correlation for ORAC-FL and hydroxyl radicals. Both varieties of guava leaf extract showed excellent antioxidant properties.

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Antioxidant activities of aqueous extracts from three cultivars of guava leaf

Cited by 14 publications

References 25 publications

Phytochemical screening and in vitro radical scavenging activities of “Gola” guava fruit and leaf extracts

Phytochemical screening and in vitro radical scavenging activities of “Gola” guava fruit and leaf extracts

Composition, Antioxidant, and Cytotoxic Activities of the Essential Oils from Fresh and Air‐Dried Aerial Parts of Pallenis spinosa

Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.

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