Antioxidant Activities and Total Phenolic and Flavonoid Content Variations of Leaf Extracts of Laurus Nobilis L. From Morocco

Abstract

Objective: The present study was undertaken to determine antioxidant activity and total phenolic and flavonoid content of ethanol, methanol, ethyl acetate, and aqueous extracts of the leaves of Laurus nobilis L.Methods: Antioxidant properties were measured using three tests: Free radical scavenging activity against 2,2-diphenyl picrylhydrazyl (DPPH), reduction of molybdate, and reducing (Fe3+/Fe2+) power. Total phenolic and flavonoid content was measured by Folin–Ciocalteu and rutin reagent, respectively.Results: The results showed that both the ethanol and water extracts had greater antioxidant activity than ethyl acetate. Aqueous extract exhibited a higher DPPH radical scavenging and reducing molybdate 545.83±5.89 mg equivalent to ascorbic acid/g dry extract. The strong antioxidant activity of water extract was probably due to its high content of phenols. Furthermore, ethanol and water extracts showed higher total flavonoid content with the values: 153.33±3.59 and 127.25±2.60 mg equivalent rutin/g dry extract, respectively.Conclusion: Our results suggested a potent and excellent antioxidant activity of L. nobilis L. extracts of Morocco. Further research has to be done to isolate the pure bioactive compound and could be considered as a potential source of biomolecules for pharmaceutical and food industry.

In vitro evaluation of antioxidant, antineurodegenerative and antidiabetic activities of Ocimum basilicum L., Laurus nobilis L. leaves and Citrus reticulata Blanco peel extracts

“…Methanolic extract of L. nobilis possessed effective reducing power in the study of Pacifico et al (2014), while in our research it was less potent comparing to acetonic extract. Water extract of Moroccan bay leaves exhibited the highest DPPH radical scavenging activity comparing to other tested extracts, which was probably due to its high content of phenols (Taroq et al, 2018). Our results showed higher antioxidant activity of acetonic extracts than methanolic extracts.…”

“…In the case of bay leaves, the yield of acetonic extract of commercial sample (31.06%) was noticeable higher comparing to other samples. On the contrary, methanolic extracts of Moroccan bay leaves showed the highest yield (22%) comparing to other applied solvents (Taroq et al, 2018). Mandarin peel showed the highest yield in methanolic extracts of both commercial and naturally cultivated samples (23.07 and 23.77%) (Table 1).…”

“…(2017) tested mandarin peels with different extraction methods and extraction solvents, and achieved the highest yield Kivrak et al (2017) have found that ethanolic extract of L. nobilis possess higher TFC than water extract, which is similar to our results, with noticeable higher TFC in methanolic extracts. Likewise, the ethanolic extract of bay leaves from Morocco had the highest TFC (Taroq et al, 2018). Zhang et al (2014) notified that TFC varied from 6.28 to 20.66 mg RE/g for methanolic extracts of peels of Chinese mandarin genotypes.…”

Formulasi dan evaluasi karakter fisik sediaan gel ekstrak etanol daun salam (Syzygium polyanthum)

“…Senyawa flavonoid dalam daun salam juga berperan sebagai antioksidan. Senyawa ini dapat melindungi tubuh dari serangan radikal bebas (Taroq et al, (2018).…”

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“…The free radical scavenging activity of the extracts, in our case, could be attributed to the phenolic content. According to the literature, phenol compounds can contribute to the antioxidant potent 9,36,37 and they are considered as anti-cancer, anti-inflammatory, antiviral, and anti-bacterial agents due to their antioxidant and free radical scavenging properties 38 .…”