Antioxidant Activities and Protective Effects of Dendropachol, a New Bisbibenzyl Compound from Dendrobium pachyglossum, on Hydrogen Peroxide-Induced Oxidative Stress in HaCaT Keratinocytes

Abstract

Five compounds including a new bisbibenzyl named dendropachol (1) and four known compounds (2–5) comprising 4,5-dihydroxy-2,3-dimethoxy-9,10-dihydrophenanthrene (2), gigantol (3), moscatilin (4) and 4,5,4′-trihydroxy-3,3′-dimethoxybibenzyl (5) were isolated from a methanolic extract of Dendrobium pachyglossum (Orchidaceae). The chemical structures of the isolated compounds were characterized by spectroscopic methods. Dendropachol (1) was investigated for its protective effects on hydrogen peroxide (H2O2)-induced oxidative stress in HaCaT keratinocytes. Compound 1 showed strong free radical scavenging compared to the positive control. For the cytoprotective effect, compound 1 increased the activities of GPx and CAT and the level of GSH but reduced intracellular reactive oxygen species (ROS) generation and accumulation. In addition, compound 1 significantly diminished the expression of p53, Bax, and cytochrome C proteins, decreased the activities of caspase-3 and caspase-9, and increased Bcl-2 protein. The results suggested that compound 1 exhibited antioxidant activities and protective effects in keratinocytes against oxidative stress induced by H2O2.

Gigantol Alleviates IL-1β-Induced Inflammation and Catabolism in Mouse Osteoarthritis via PI3K/Akt/NF-κB Pathways In Vivo and In Vitro

“…Gigantol, a biphenolic compound found in Dendrobium species, has been reported exhibit numerous biological functions including antitumor effects in human liver and lung cancer, anti-in ammatory effects, and anti-oxidant effects. 23,27,29,35 Nevertheless, as far as we know, there is currently no research exploring the therapeutic effect of gigantol on OA chondrocytes dysfunction. In the present study, we demonstrated the therapeutic effect of gigantol on IL-1β-induced chondrocytes and OA mice model.…”

“…22 Currently, a great number of studies implies that gigantol could exert multiple biological effects e.g., antioxidant, bene t of diabetic nephropathy, and restraint some kinds of cancers. [23][24][25] Moreover, previous evidences indicated that crude extracts from Dendrobium plants motivate the production of collagen in human dermal broblasts and inhibited MMP. 26 Deciga-Campos, M. et al suggested gigantol decreased carrageenan induced in ammation in rats.…”

Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)

“…The 50% ethanolic extracts of Centella were dissolved with DMSO to produce a 1 mg/mL stock solutions. The treatment protocol was slightly modified from our previous in vitro experiments on skin cells 21 , 22 . To observe the preventive activities of Centella extracts against H 2 O 2 -induced cytotoxicity, BJ cells were pre-treated with the Centella extracts (final concentration, 15–60 µg/mL; 0.5% DMSO) for 24 h prior to H 2 O 2 treatment.…”

Protective Effect and Potential Antioxidant Role of Kakadu Plum Extracts on Alcohol-Induced Oxidative Damage in HepG2 Cells

“…Various assays are available to quantify antioxidant capacity and, from these, we chose DPPH and the potassium ferricyanide reducing power assay to evaluate the antioxidant potential of our samples, expressed as gallic acid equivalents (GAE) and ascorbic acid equivalents (AAE). These assays are used widely to determine the antioxidant properties of compounds as free radical scavengers or hydrogen donors [50], and the results are shown in (Table 4) The results of the DPPH assay revealed that the antioxidant capacity of the fruits ranged from 1.16 ± 0.02 to 239.7 ± 8.17 mg/g, expressed as GAE, and varied from 2.47 ± 0.05 to 124.4 ± 4.34 mg/g, expressed as AAE. Similarly, the reducing power assay recorded that, in comparison with all fruit samples, KKD-NT and KKD-WA showed the highest antioxidant properties, ranging from 6.65 ± 0.44 to 179.6 ± 5.35 mg GAE/g DW and from 14.08 ± 0.83 to 74.4 ± 2.06 mg AAE/g DW.…”