Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)

Abstract

Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin. The use of phytochemicals that can promote capacity of fibroblasts to combat oxidative stress is an attractive strategy to prevent skin aging and promote skin beauty. Centella asiatica has been used to treat multitude of diseases for centuries. Previous investigations demonstrated that extracts from C. asiatica have a broad range of beneficial activities through their antioxidant activity. Hence, the extract from this medicinal plant could be a great candidate for anti-skin-aging agent. Callus culture offers a powerful platform for sustainable, rapid and large-scale production of phytochemicals to serve extensive demands of pharmaceutical and cosmeceutical industries. Here, we demonstrated the application of callus culture of Centella asiatica to produce bioactive metabolites. The 50% ethanolic extract of callus culture has distinctive features of chemical compositions and biological profiles. Information from HPTLC-DPPH and HPLC analysis suggested that the callus extract comprises distinctive antioxidant compounds, compared with those isolated from authentic plant. Moreover, results from cell culture experiment demonstrated that callus extract possesses promising antioxidant and anti-skin-aging activities. Pre-treatment with callus extract attenuated H2O2-induced-cytotoxicity on human dermal fibroblasts. The results from RT-qPCR clearly suggested that the upregulation of cellular antioxidant enzymes appeared to be major contributor for the protective effects of callus extract against oxidative stress. Moreover, supplementation with callus extract inhibited induction of matrix metalloprotease-9 following H2O2 exposure, suggesting its potential anti-skin-aging activity. Our results demonstrate the potential utility of C. asiatica callus extract as anti-skin-aging agent in cosmeceutical preparations.

Some Antioxidant Properties of Components from the Flower of Ochna integerrima and Their Beneficial Effects on HaCaT Keratinocytes and in Silico Analysis on Tyrosinase

“…HPTLC‐DPPH⋅ was performed to screen the antioxidant activity of the tested samples [40] . The plate was incubated with 0.02 % DPPH⋅‐MeOH solution for 2 .…”

“…HPTLC-DPPH * was performed to screen the antioxidant activity of the tested samples. [40] The plate was incubated with 0.02 % DPPH * -MeOH solution for 2. Antioxidant activity was indicated by the yellowish band on the purple background under white light.…”

Ameliorative effect of gel combination of Centella asiatica extract transfersomes and rosemary essential oil nanoemulsion against UVB-induced skin aging in Balb/c mice

“…The main chemical components of REO including -pinene, camphor, 1-8-cineole, and verbenone, have been reported to exhibit antioxidant, antimicrobial, and anti-aging activities 18,19 . Many properties make CA and REO potential candidates in preventing skin aging 20,21 . However, their use in preclinical and clinical pharmacokinetics is limited due to physicochemical characteristics such as a high molecular weight and instability, that impede skin absorption and low bioavailability after topical applications 12,22 .…”

UBIAD1 alleviates ferroptotic neuronal death by enhancing antioxidative capacity by cooperatively restoring impaired mitochondria and Golgi apparatus upon cerebral ischemic/reperfusion insult

“…The mitochondrial oxidative stress-inducing agent (H 2 O 2 ) was applied to UBIAD1-overexpression neurons to modulate the antioxidant capacities [ 39 ]. Then, oxidative stress levels were determined in UBIAD1-overexpression neurons.…”