Antioxidant Activities and Heavy Metal Contents of Castanea Sativa Honey

Abstract

<p>Honey is one of the most valuable foods in terms of its antioxidant nature and antioxidant activity. In this study, their botanic origins, total flavonoid content, total phenol content, the hydrogen peroxide scavenging activity (HPSA) (in terms of SC50 (µg mL-1)), ferric reducing antioxidant power capacity (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (in terms of SC50), metal-chelating activity (MCA) (%) and heavy metal amounts were examined to determine the qualities of honey samples that gathered from Giresun city of Black Sea region in Turkey. According to the melissopalynological analysis, the botanic origins of the samples are Castanea sativa (Chestnut) Miller and uni-floral. The HPSA, FRAP, DPPH, MCA (%), total phenol content (TPC), and total flavonoid content (TFC) were found between 251.99±0.48-258.64±1.22 µg mL-1, 71.34±0.09-73.71±0.20 %, 584.86±0.06-595.04±0.29 µg mL-1, 36.73±0.00-36.86±0.09 %, 93.82±1.05-173.15±2.46 mg GAE 100 g-1 and 5.51±0.19-8.29±0.05 mg CAE/100 g, respectively. For comparison of these results, Butylated Hydroxy Anisole (BHA), Butylated Hydroxy Toluene (BHT) and α-Tocopherol (TOC) were used as standard antioxidant compounds. In addition, it was observed that honey samples were been contaminated with most of the heavy metals (Al, Ca, Cr, Cu, Fe, Li, Mg, Mn, Ni, Rb, and Zn) to a lesser or greater extent, whereas others (Te, Tl, and U) were never detected in these samples. However, Cd and Cs were detected only in one sample and Pb in another sample Finally, the results indicate that although honey is an important source of nutrients, nevertheless it could be affected by environmental pollution.</p>

The chemical composition of pollen, staminate catkins, and honey of Castanea sativa Mill.

“…Fe contents in honey samples were found in amounts from 6.280 mg.kg -1 to 20.515 mg.kg -1 and showed similarity to the other study in Turkey (Yücel and Sultanoğlu, 2013), and higher than performed in Portugal (0.70 -7.06 mg.kg and New Zealand (0.67 -3.39 mg.kg -1 ) (Vanhanen, Emmertz and Savage, 2011). Kızılpınar Temizer et al (2018) determined Mn contents in amounts from 21.58 to 26.52 mg.kg -1 , which are higher compared to our honey sample. The mineral content in honey is also related to the geographical origin, therefore it is possible to be used to differentiate between honey of different geographical origins (Davies, 1975;Wang and Li, 2011).…”

“…Kızılpınar Temizer et al (2018) determined heavy metal content in three unifloral honey samples of Castanea sativa from the Black Sea region in Turkey. Fe contents in honey samples were found in amounts from 6.280 mg.kg -1 to 20.515 mg.kg -1 and showed similarity to the other study in Turkey (Yücel and Sultanoğlu, 2013), and higher than performed in Portugal (0.70 -7.06 mg.kg and New Zealand (0.67 -3.39 mg.kg -1 ) (Vanhanen, Emmertz and Savage, 2011).…”

Bitlis’ten alınan bazı balların Botanik Orijini ve Antioksidan Aktivitesi

“…Nayik and Nanda (2016) declared that total flavonoid content 8-17 mg QE/100 g. These results are higher than our results. Temizer et al (2018) determined that total flavonoid contents were 5.51±0. Sherin et al (2015) determined the metal-chelating activities for honey samples as 54.2%.…”

“…There are many analytical methods for definition of the antioxidant capacity of honey, a lot of analytical methods are been used. The most generally experiments are total phenolic and flavonoid contents, DPPH, HPSA, FRAP, ABTS, FICA (Al-Hindi Shehata, 2014;Kıvrak and Kıvrak, 2017;Temizer et al, 2018;Temizer et al, 2019).…”