Antiobesity Effect of Dead Lactobacillus plantarum nF1 on High-Fat Diet-Induced C57BL/6 Mice

Abstract

The anti-obesity effects of dead Lactobacillus plantarum nF1 (nLp), live Lactobacillus plantarum pF1 (pLp), or live Lactobacillus plantarum PNU (PNU) were studied on C57BL/6 mice fed a 45% high-fat diet. The body, liver, and epididymal fat weights of mice fed nLp were significantly lower than those of mice in the pLp, PNU, or control groups (P<0.05). Serum triglyceride, total cholesterol, glucose, and insulin levels were significantly lower in the nLp, pLp, or PNU groups than in control, and these levels were significantly lower in the nLp group than in the pLp and PNU groups (P<0.05). On the other hand, adiponectin and HDL-cholesterol levels were significantly higher in the nLp group than in the pLp, PNU, and control groups. Histological observations indicated that nLp reduced fat accumulation in liver tissue. Furthermore, L. plantarum strains significantly reduced the mRNA expressions of SREBP-1c, increased the mRNA levels of PPAR-α, CPT-1, and ACO in liver tissues, and significantly increased the mRNA levels of PPAR-α, and CPT-1 in epididymal fat tissues. In particular, the mRNA expressions of PPAR-α and CPT-1 in liver and epididymal fat tissues were highest in the nLp group. These results suggest that nLp (dead L. plantarum) has a greater anti-obesity effect on high-fat diet-induced mice than the live strain (pLp) and a live L. plantarum PNU strain.

Increased Anticancer Activity of Organic Kimchi with Starters Demonstrated in HT-29 Cancer Cells

“…In addition, postbiotic metabolites produced from LP caused selective antiproliferative effects and apoptosis on cancer cells while preserving normal cells, and cell proliferation inhibitory effects and total phenol content on HT-29 colorectal cancer cells in soymilk fermented with LAB increased [10,11]. Dead nanometric Lactiplantibacillus plantarum (nF1), produced by the heat treatment of LP, is less than 1 µm in size and is absorbed faster through the Peyer's patches of M cells in the intestine, and it has immune-enhancing and anti-obesity effects [12]. However, the functional study of kimchi co-produced with LP, LM, and nF1 has not been comprehensively conducted to date.…”