Antinociceptive Profiles of Crude Extract from Roots of Angelica gigas NAKAI in Various Pain Models

Abstract

To characterize the antinociceptive profiles of Angelica gigas NAKAI (ANG; Korean angelica), methanol extract from the dried roots of ANG was made and mice were administered orally at the various doses (from 0.25 to 3 g/kg). ANG produced the increased latencies of the tail-flick and hot-plate paw-licking responses in a dose-dependent manner. In acetic acid-induced writhing test, ANG dose-dependently decreased writhing numbers. Moreover, the cumulative response time of nociceptive behaviors induced by intraplantar formalin injection was reduced during both the 1st and the 2nd phases in a dose-dependent manner in ANG-treated mice. Furthermore, oral administration of ANG did not cause licking, scratching and biting responses induced by TNF-alpha (100 pg), IFN-gamma (100 pg) or IL-1beta (100 pg) injected intrathecally (i.t.), especially at higher dose (3 g/kg). Additionally, in ANG treated mice, the cumulative nociceptive response time for i.t. administration of substance P or capsaicin was dose-dependently diminished. Finally, nociceptive responses elicited by i.t. injection of glutamate (20 microg), N-methyl-D-aspartic acid (60 ng), alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (13 ng) or kainic acid (12 ng) were decreased by oral administration of ANG. Our results suggest that ANG produces antinociception via acting on the central nervous system and shows antinociceptive profiles in various pain models, especially inflammatory pain.

ESP-102, a standardized combined extract of Angelica gigas, Saururus chinensis and Schizandra chinensis, significantly improved scopolamine-induced memory impairment in mice

“…In addition, decursin, a major coumarin derivative, greatly improved scopolamine-induced amnesia in both passive avoidance and Morris water maze performance tests through AChE inhibition in vivo (Kang et al, 2003). It was also recently reported that coumarins and/or extract of A. gigas exhibited anti-amnesic (Yan et al, 2004), antitumor , antinociceptive (Choi et al, 2003), anti-bacterial and antiplatelet aggregation (Lee et al, 2003d) activities. In order to obtain a more effective remedy for the treatment of memory deficiency than exclusively using A. gigas roots, we combined several extracts of Oriental herbs and changed the composition based on both traditional references and the results of our previous work.…”

CML-1 inhibits TNF-α-induced NF-κB activation and adhesion molecule expression in endothelial cells through inhibition of IkBα kinase

“…The anti-inflammatory properties of Achyranthis Radix (Ida et al, 1998), Atractylodis Rhizoma (Resch et al, 1998) Cinnamomi Cortex Spissus (Hong et al, 2002), Glycyrrhizae Radix (Baltina, 2003), Poria cocos (Schinella et al, 2002), Paeoniae Radix (Xue et al, 2000), Rehmanniae Radix Preparata (Kim et al, 1999), and Zingiber officinale (Shen et al, 2003) have been mentioned. The analgesic properties of Angelicae Gigantis Radix (Choi et al, 2003), and Eucommiae Cortex (Hong et al, 1988) were earlier investigated in various inflammatory pain models. Therefore, these results suggest that CML-1 may have synergic therapeutic effects from each herb on inflammatory diseases.…”

The effect of extract from leaves and stalks of Angelica gigas on the innate immunity

“…The extraction method was modified from the article of Choi et al (2003). Crude extract was prepared as follow.…”