CML-1 inhibits TNF-α-induced NF-κB activation and adhesion molecule expression in endothelial cells through inhibition of IkBα kinase

Mo, Seong-Ji; Son, Eunwha; Lee, Sungryul; Lee, Sun-Mee; Shin, Dae-Hee; Pyo, Suhkneung

doi:10.1016/j.jep.2006.07.006

Cited by 45 publications

(25 citation statements)

References 35 publications

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“…However, pretreatment with astaxanthin decreased nuclear p50 activity. Similarly, a purified extract of herbs commonly used for treatment of inflammatory diseases in Asia, also was shown to downregulate NF-κB activation in HUVEC stimulated with 10 ng/mL TNF-α (Mo et al, 2007).…”

Section: Discussionmentioning

confidence: 95%

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Chew¹,

Mathison²,

Kimble³

et al. 2013

AJAFST

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Oxidative stress and inflammation are strongly linked to the development of cardiovascular disease (CVD). As a potent antioxidant, astaxanthin may downregulate inflammation-associated factors involved in endothelial dysfunction and CVD progression. We studied the possible protective effects of astaxanthin against endothelial dysfunction in human umbilical vein endothelial cells (HUVEC) induced with hydrogen peroxide (H2O2). Cells were pre-incubated with 0, 0.01, 0.1, or 1.0 μmol/L astaxanthin for 48 h, then oxidative stress induced with 100 μmol/L H2O2 overnight. Uptake kinetics of astaxanthin showed a time-dependent uptake of astaxanthin (1.0 μmol/L) by HUVEC over the 48 h incubation period. Oxidative stress induction with H2O2 in HUVEC decreased intracellular antioxidant activity and increased the production of inflammatory biomarkers and reactive oxygen species (ROS). In contrast, pre-incubation of HUVEC with astaxanthin prior to H2O2 stress increased (P < 0.05) superoxide dismutase activity and decreased ROS, prostaglandin E2, leukotriene B4, NO, IL-8, and IFN- production. Pre-treatment with astaxanthin also downregulated the transcriptional activation of NF-κB and activator protein-1, thereby inhibiting downstream production of inflammatory mediators and cytokines. Therefore, astaxanthin protects against factors initiated by H2O2 addition, likely by scavenging ROS required for transcriptional activation and inhibiting the production of inflammatory biomarkers involved in endothelial dysfunction and CVD.Abbreviations: AP-1: activator protein-1; CVD: cardiovascular disease; CS: culture supernatant; EGM-2: endothelial growth medium-2; GPx: glutathione peroxidase; H2O2: hydrogen peroxide; HUVEC: human umbilical vein endothelial cell; LTB4: leukotriene B4; PGE2: prostaglandin E2; ROS: reactive oxygen species; SOD: superoxide dismutase; THF: tetrahydrofuran.

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Section: Discussionmentioning

confidence: 95%

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Chew¹,

Mathison²,

Kimble³

et al. 2013

AJAFST

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“…* and **, significantly different from positive control (P \ 0.05 and 0.01, respectively) of inflammatory diseases in Asia (Achyranthis Radix, Angelicae Gigantis Radix, Cinnamomi Cortex Spissus, Eucommiae Cortex, Glycyrrhizae Radix, Hoelen, Lycii Fructus, Paeoniae Radix, Rehmanniae Radix Preparata and Atractylodis Rhizoma, Zingiberis Rhizoma, Zizyphi Semen, Acori Graminei Rhizoma). This complex mixture inhibits TNF-a-induced IjB kinase activation, subsequent degradation of IjBa, and nuclear translocation of NK-jB that may explain the ability of CML-1 to suppress inflammation [36]. Finally, an extract from stem bark of Cinnamomum cassia Blume (Lauraceae) was discovered to exert an inhibitory effect on lipopolysaccharide (LPS)-induced NF-JB transcriptional activity.…”

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway

et al. 2010

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Azadirachta indica (neem tree) is used in traditional Indian medicine for its pharmacological properties including cancer prevention and treatment. Here, we studied a neem extract's anti-inflammatory potential via the nuclear factor-jB (NF-jB) signaling pathway, linked to cancer, inflammation, and apoptosis. Cultured human leukemia cells were treated with a methanolic neem leaf extract with or without tumor necrosis factor (TNF)-a stimulation. Inhibition of NF-jB activity was demonstrated by luciferase assay and electrophoretic mobility shift assay (EMSA). Inhibition of viability by neem extracts was assessed by luminescent assays. Western blot analysis allowed assessing the inhibitory effect of the neem extract on TNF-a-induced degradation of inhibitor of jB (IjB) and nuclear translocation of the NF-jB p50/p65 heterodimer. Inhibition of IjB kinase (IKK) activity was shown as well as the effect of neem extract on the induction of apoptotic cell death mechanisms by nuclear fragmentation analysis and flow cytometry analysis. In conclusion, our data provide evidence for a strong effect of the neem extract on proinflammatory cell signaling and apoptotic cell death mechanisms, contributing to a better understanding of the mechanisms triggered by Azadirachta indica.

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“…The cell surface expression of the adhesion molecules on the muscle cell monolayers was quantified by ELISA using a modification of the methods described previously [15]. VSMCs were seeded at a concentration of 2 × 10 4 cells/well in 96-well gelatin-coated plates, cultured to confluence and pre-treated with stereocalpin A (0.1-10 μg/ml) for 2 h at 37°C.…”

Section: Determination Of Cell Surface Expression Of Adhesion Moleculmentioning

confidence: 99%

“…It is well documented that NF-κB is ubiquitous transcription factor that plays a crucial role in adhesion molecule expression [15,[18][19][20]. We therefore examined the effect of stereocalpin A on NF-κB transcriptional activation.…”

Section: Inhibition Of Tnf-α-induced Nf-κb Activation By Stereocalpin Amentioning

confidence: 99%

Stereocalpin A inhibits the expression of adhesion molecules in activated vascular smooth muscle cells

Byeon

Park

Yim

et al. 2012

International Immunopharmacology

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CML-1 inhibits TNF-α-induced NF-κB activation and adhesion molecule expression in endothelial cells through inhibition of IkBα kinase

Cited by 45 publications

References 35 publications

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway

Stereocalpin A inhibits the expression of adhesion molecules in activated vascular smooth muscle cells

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