Antinociceptive Effects of Cardamonin in Mice: Possible Involvement of TRPV1, Glutamate, and Opioid Receptors

Abstract

Pain is one of the most common cause for hospital visits. It plays an important role in inflammation and serves as a warning sign to avoid further injury. Analgesics are used to manage pain and provide comfort to patients. However, prolonged usage of pain treatments like opioids and NSAIDs are accompanied with undesirable side effects. Therefore, research to identify novel compounds that produce analgesia with lesser side effects are necessary. The present study investigated the antinociceptive potentials of a natural compound, cardamonin, isolated from Boesenbergia rotunda (L) Mansf. using chemical and thermal models of nociception. Our findings showed that intraperitoneal and oral administration of cardamonin (0.3, 1, 3, and 10 mg/kg) produced significant and dose-dependent inhibition of pain in abdominal writhing responses induced by acetic acid. The present study also demonstrated that cardamonin produced significant analgesia in formalin-, capsaicin-, and glutamate-induced paw licking tests. In the thermal-induced nociception model, cardamonin exhibited significant increase in response latency time of animals subjected to hot-plate thermal stimuli. The rota-rod assessment confirmed that the antinociceptive activities elicited by cardamonin was not related to muscle relaxant or sedative effects of the compound. In conclusion, the present findings showed that cardamonin exerted significant peripheral and central antinociception through chemical- and thermal-induced nociception in mice through the involvement of TRPV1, glutamate, and opioid receptors.

Peripheral Analgesic Effect and Possible Mechanisms of Ferulic Acid

“…13 Acetic acid-induced writhing test, which is used to evaluate the antinociceptive and anti-inflammatory features of potential analgesic agents in visceral pain, was used to evaluate the peripheral analgesic effect of ferulic acid and the contribution of NO/cGMP/PKG/KATP pathway to this effect in mice. 23 Writhing test is a chemical procedure which is used to arouse peripheral pain in mice with the injection of irritant substances such as acetic acid. The writhing behavior is characterized by contractions in abdominal muscles and backward extension of back extremities and rubbing of the stomach on the floor.…”

Cardamonin prevents degranulation in fMLP and opsonized zymosan activated neutrophils by attenuating myeloperoxidase release and superoxide generation

“…Cardamonin, chemically known as (2', 4'-dihydroxy-6'methoxychalcone) is a naturally occurring chalcone, (Bheemasankara et al, 1976). Cardamonin is known to exhibit many pharmacological activities including anti-inflammatory, anti-mutagenic, antineoplastic, anti-proliferative, anti-tumor, anti-HIV, in vitro vasorelaxant, hypoglycemic, anti-infectious and anti-platelet activities (Ping et al, 2018). It is also known to possess potential antioxidant effect.…”

Anxiolytic Potency of Cardamonin Mediated through Brain GABAergic System

“…Cardamonin, chemically known as (2', 4'-dihydroxy-6'methoxychalcone) is a naturally occurring chalcone, foremost isolated from seeds of Amomum subulatum and later from other plant species, such as Alpinia henryi, Alpinia katsumadai, Alpinia rafflesiana, Boesenbergia pandurata and Campomanesia adamantium 5 . Cardamonin is known to exhibit many pharmacological activities including antiinflammatory, anti-mutagenic, anti-neoplastic, antiproliferative, anti-tumor, in vitro anti-HIV, vasorelaxant, hypoglycemic, anti-infectious and anti-platelet activities 6 . It is also known to possess potential beneficial effects on various neurological disorders including anti-nociceptive property 6 , neuroprotection of PC12 cells against oxidative damage 7 and ameliorative effect on hyperalgesia and allodynia in a mouse model of chronic constriction injuryinduced neuropathic pain 8 .…”

“…Cardamonin is known to exhibit many pharmacological activities including antiinflammatory, anti-mutagenic, anti-neoplastic, antiproliferative, anti-tumor, in vitro anti-HIV, vasorelaxant, hypoglycemic, anti-infectious and anti-platelet activities 6 . It is also known to possess potential beneficial effects on various neurological disorders including anti-nociceptive property 6 , neuroprotection of PC12 cells against oxidative damage 7 and ameliorative effect on hyperalgesia and allodynia in a mouse model of chronic constriction injuryinduced neuropathic pain 8 . The quest for potential health applications of cardamonin particularly in relation to neuroprotection seems to grow in the near future as shown clearly by the increasing number of ongoing research.…”