Anxiolytic Potency of Cardamonin Mediated through Brain GABAergic System

Rajput, Mithun Singh; Rathore, Devashish; Dahima, Rashmi

doi:10.22270/jddt.v9i1.2322

Cited by 2 publications

(2 citation statements)

References 6 publications

(8 reference statements)

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“…Ltd. Bengaluru, India). Cardamonin was freshly prepared by dissolving in DMSO, tween 20 and distilled water at a ratio of 5:5:90 (v/v) (Rajput et al, 2019). All other drugs were dissolved in distilled water.…”

Section: Drugs and Solutionsmentioning

confidence: 99%

Cardamonin prevents degranulation in fMLP and opsonized zymosan activated neutrophils by attenuating myeloperoxidase release and superoxide generation

Rajput¹,

Rathore²,

Dahima³

2019

Asian J Pharm Pharmacol

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Background: Neutrophils are one of the components of innate immune system that defend against infectious microorganisms through various antimicrobial capabilities, including microorganism phagocytosis and by releasing bactericidal agents from intracellular secretory granules into the phagosome or to the cell exterior. Activated neutrophils represent the main source of myeloperoxidase (MPO), superoxide (SO) and subsequently derived oxygen metabolites. The current study was carried out with the aim to investigate the effect of cardamonin on N-Objective: formyl-methionyl-leucyl-phenyl-alanine (fMLP) and opsonized zymosan (OZ) stimulated SO generation in neutrophils and their degranulation measured as MPO release. pectrophotometry was used Materials and Methods: S to evaluate the effect of cardamonin (0.1 to 100.0 OZ (0.5 mg/ml) or fMLP (0.1 μmol/l) on μmol/l) stimulated SO generation and MPO release in neutrophil cells. Superoxide formation was measured in isolated neutrophils as superoxide dismutase inhibitable reduction of cytochrome c and the activity of MPO was assayed by determining the oxidation of-dianisidine in the presence of hydrogen peroxide. It is evident from one way ANCOVA o Results: followed by post-hoc test that cardamonin dose-dependently (1.0 to 100.0 significantly decreased (p < 0.05) SO μmol/l) generation and MPO release after each stimulus (fMLP or OZ). Our results indicate that cardamonin Conclusion: could support resolution of inflammation through decreased activity of neutrophils, respiratory burst and i.e. degranulation by inhibiting MPO release and by decreasing the generation of SO and the subsequently derived reactive oxygen species.

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Section: Drugs and Solutionsmentioning

confidence: 99%

Cardamonin prevents degranulation in fMLP and opsonized zymosan activated neutrophils by attenuating myeloperoxidase release and superoxide generation

Rajput¹,

Rathore²,

Dahima³

2019

Asian J Pharm Pharmacol

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“…More than 450 million people suffer from psychiatric or behavioral disorders, which add up to 12.3 percent of the global disease burden and are expected to increase to 15 percent by 2020 (Rajput et al., 2019). Currently, the most psychiatric conditions that raise morbidity in the world tend to be anxiety and depression.…”

Section: Introductionmentioning

confidence: 99%

Bioactive metabolites of Blumea lacera attenuate anxiety and depression in rodents and computer‐aided model

Hossen¹,

Reza²,

Amin³

et al. 2021

Food Science & Nutrition

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Blumea lacera is an edible plant with imperative medicinal values. However, the anxiolytic and antidepressant roles of B. lacera have not been well‐explained. Therefore, the current study aims to explore the impending bioactive metabolites and roles of B. lacera methanol leaf extract (Me‐BLL) in attenuating anxiety and depression through several experimental and computer‐aided approaches. The chemical characterization of Me‐BLL was performed through standard phytochemical and GC‐MS analyses. To explore the neuropharmacological insights, Swiss albino mice were treated with Me‐BLL at doses of 200–400 mg/kg, p.o. The anxiolytic effects were observed employing elevated plus maze (EPM), light–dark box (LDB), and hole‐board (HBT) tests, while antidepressant effects were evaluated using forced swimming (FST) and tail suspension tests (TST). Diazepam (1 mg/kg, i.p.) and fluoxetine HCl (20 mg/kg, p.o.) were used as the reference standard. The phytochemical analyses revealed several bioactive metabolites, including higher contents of total phenolics and flavonoids. The EPM and LDB tests demonstrated an increased time spent in open arms and light box, and the HBT showed an increased number of head dipping, indicating the anxiolytic effects of Me‐BLL. The TST and FST revealed a decrease in immobility time, meaning the persuasive antidepressant effects. The antioxidative effects of Me‐BLL have also been observed prominently. Correspondingly, the computer‐aided investigation confirmed several bioactive lead molecules. Specifically, thymol and cuminol revealed potential anxiolytic and antioxidant effects, while stigmast‐5‐en‐3.beta.‐ol and gamma‐sitosterol possessed promising antidepressant effects. Taken these results as a base, the plant has imperative potentials in managing anxiety and depression‐like disorders.

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Anxiolytic Potency of Cardamonin Mediated through Brain GABAergic System

Cited by 2 publications

References 6 publications

Cardamonin prevents degranulation in fMLP and opsonized zymosan activated neutrophils by attenuating myeloperoxidase release and superoxide generation

Cardamonin prevents degranulation in fMLP and opsonized zymosan activated neutrophils by attenuating myeloperoxidase release and superoxide generation

Bioactive metabolites of Blumea lacera attenuate anxiety and depression in rodents and computer‐aided model

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