Antinociceptive effect of novel trihalomethyl-substituted pyrazoline methyl esters in formalin and hot-plate tests in mice

Antinociceptive activities of some triazole and pyrazoline moieties-bearing compounds

“…The findings obtained in this investigation proved the hypothesis that the tested triazole-pyrazoline derivative compounds have significant antinociceptive activities in mice and supported the previous studies reporting the analgesic activities of the compounds containing triazole and/or pyrazoline groups (Turan-Zitouni et al, 2001;Godoy et al, 2004;Tabarelli et al, 2004;Dündar et al, 2007;Salgin-Gökşen et al, 2007;Milano et al, 2008;Kaplancıklı et al, 2009;Karthikeyan, 2009;Girisha et al, 2010). …”

“…Besides peripherally mediated antinociceptive action, centrally mediated antinociceptive activities have also been reported for some pyrazoline derivative compounds (Godoy et al, 2004;Tabarelli et al, 2004;Milano et al, 2008;Kaplancıklı et al, 2009;Girisha et al, 2010). Activation of opioidergic system (Tabarelli et al, 2004;Prokopp et al, 2006;Milano et al, 2008;Kaplancıklı et al, 2009), decrease of on/off cell firing in the periaqueductal gray (Tabarelli et al, 2004), and activation of spinal serotonergic and adrenergic systems (Godoy et al, 2004) have been suggested as possible mechanisms of central analgesic action.…”

Tabernaemontana catharinensis ethyl acetate fraction presents antinociceptive activity without causing toxicological effects in mice

“…Animals received a subcutaneous administration of formalin (2.5%, v/v; 20 µl/paw) into their dorsal surface of the right hind paw (Milano et al, 2008a). Immediately after the formalin injection, the animals were individually placed in an observation chamber made of glass with a transparent floor.…”

Antinociceptive activity of Mirabilis jalapa in mice

“…In order to investigate the possible effect of Eta or CrdL over the forced and spontaneous locomotor performance was tested in the rotarod and open-field tests, respectively (Milano et al, 2008). Twenty-four hours before the tail-flick test, all animals were trained in the rotarod (3.7 cm in diameter, 8 r.p.m.)…”

“…In this study, mice were fasted for 18-24 h (water ad libitum) before the gastrointestinal transit analyses, as described previously (Milano et al, 2008). The animals were treated with Eta (10 mg/kg, p.o.…”