Antinociceptive constituents from Saccharum officinarum L. juice

Abstract

This work aimed to investigate the main components of methanol fractions (MFSC and MFSCf) from Saccharum officinarum L. juice and their in vivo antinociceptive potential.After LC-ESI-MS and ESI-MS/MS analysis, phenolic compounds, such as dicaffeoylquinic acid, schaftoside, vicenin-2, stilbene glycoside and the major compound tricin-7-O-(2''-α-Lrhamnopyranosyl)-α-D-galacturonide (1), were identified. MFSC and MFSCf significantly inhibited nociceptive responses in classical mice pain models. The isolated flavone, 1, inhibited strongly the neurogenic phase in formalin test without interfering with the inflammatory one. The co-administration of the opioid antagonist, naloxone, significantly reversed the antinociceptive effects on the neurogenic phase of both methanol fractions and 1, demonstrating the involvement of the opioid system on the antinociceptive effect. This work describes for the first time the antinociceptive effect of flavonoids present in sugarcane juice, highlighting the isolation, identification and the remarkable antinociceptive effect of the flavonoid tricin-7-O-(2''-α-L-rhamnopyranosyl)-α-D-galacturonide.

Paper Spray Mass Spectrometry on the Analysis of Phenolic Compounds in Rhynchelytrum repens: A Tropical Grass with Hypoglycemic Activity

“…Tricin and luteolin 6,8-di-C-glucoside have been shown to possess a potent free-radical trapping activity, being considered a natural trapping agent for these radicals. Tricin-7-O-(200-a-L-rhamnopyranosyl)-a-D-galacturonide was also detected in R. repens with a reported antinociceptive activity [29][30][31] Research of the main components present in the aqueous extract of this plant is currently being carried out by our group to further expand the knowledge of this species' potential. Identification of such a wide range of Rhynchelytrum repens phenolic compounds reinforces the importance of this species, which should be the subject of further investigation.…”

“…Tricin and luteolin 6,8-di-C-glucoside have been shown to possess a potent free- radical trapping activity, being considered a natural trapping agent for these radicals. Tricin-7-O-(200-a-L-rhamnopyranosyl)-a-D-galacturonide was also detected in R. repens with a reported antinociceptive activity [ 29 , 30 , 31 ]…”

“… 1 Identification based on expected mass signals [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. …”

Flavonoids and fractions from Saccharum officinarum L. juice: antinociceptive agents and molecular docking evaluations with µ-opioid receptor

“…The protocol for obtaining fractions from sugarcane juice was carried out according our previous work (Gomes et al, 2020). The juice from different varieties of S. officinarum (150 mL) was autoclaved (1 atm, 121 ºC) for 20 min to eliminate contaminating microorganisms.…”