Abstract:This work aimed to investigate the main components of methanol fractions (MFSC and MFSCf) from Saccharum officinarum L. juice and their in vivo antinociceptive potential.After LC-ESI-MS and ESI-MS/MS analysis, phenolic compounds, such as dicaffeoylquinic acid, schaftoside, vicenin-2, stilbene glycoside and the major compound tricin-7-O-(2''-α-Lrhamnopyranosyl)-α-D-galacturonide (1), were identified. MFSC and MFSCf significantly inhibited nociceptive responses in classical mice pain models. The isolated flavone… Show more
“…Tricin and luteolin 6,8-di-C-glucoside have been shown to possess a potent free-radical trapping activity, being considered a natural trapping agent for these radicals. Tricin-7-O-(200-a-L-rhamnopyranosyl)-a-D-galacturonide was also detected in R. repens with a reported antinociceptive activity [29][30][31] Research of the main components present in the aqueous extract of this plant is currently being carried out by our group to further expand the knowledge of this species' potential. Identification of such a wide range of Rhynchelytrum repens phenolic compounds reinforces the importance of this species, which should be the subject of further investigation.…”
Section: Discussionmentioning
confidence: 99%
“…Tricin and luteolin 6,8-di-C-glucoside have been shown to possess a potent free- radical trapping activity, being considered a natural trapping agent for these radicals. Tricin-7-O-(200-a-L-rhamnopyranosyl)-a-D-galacturonide was also detected in R. repens with a reported antinociceptive activity [ 29 , 30 , 31 ]…”
The characterization of plant compounds with pharmacological activity is a field of great relevance in research and development. As such, identification techniques with the goal of developing new drugs or even validating the bioactive properties of extracts must be explored in order to further expand the knowledge of plant extract composition. Most works in this field employ HPLC, when exploring non-structural and cell wall carbohydrates from Rhynchelytrum repens. Phenolic compounds were studied by classical chromatography techniques and UV-vis spectrophotometry, with C-glycosylated flavonoids being detected but with no further details regarding the chemical structure of these compounds. In this work we employ paper spray ionization mass spectrometry (PS-MS) for the evaluation of the chemical profile of R. repens methanol extract. Positive ionization mode identified 15 compounds, belonging to flavonoids, fatty acids, and other classes of compounds; negative mode ionization was able to identify 20 compounds comprising the classes of quinic acids, stilbenes and flavonoids. PS-MS proved effective for the evaluation of R. repens extracts, making it possible to identify a total of thirty-five compounds. The bioactive properties attributed to R. repens were confirmed by the identification and characterization of compounds identified by PS-MS.
“…Tricin and luteolin 6,8-di-C-glucoside have been shown to possess a potent free-radical trapping activity, being considered a natural trapping agent for these radicals. Tricin-7-O-(200-a-L-rhamnopyranosyl)-a-D-galacturonide was also detected in R. repens with a reported antinociceptive activity [29][30][31] Research of the main components present in the aqueous extract of this plant is currently being carried out by our group to further expand the knowledge of this species' potential. Identification of such a wide range of Rhynchelytrum repens phenolic compounds reinforces the importance of this species, which should be the subject of further investigation.…”
Section: Discussionmentioning
confidence: 99%
“…Tricin and luteolin 6,8-di-C-glucoside have been shown to possess a potent free- radical trapping activity, being considered a natural trapping agent for these radicals. Tricin-7-O-(200-a-L-rhamnopyranosyl)-a-D-galacturonide was also detected in R. repens with a reported antinociceptive activity [ 29 , 30 , 31 ]…”
The characterization of plant compounds with pharmacological activity is a field of great relevance in research and development. As such, identification techniques with the goal of developing new drugs or even validating the bioactive properties of extracts must be explored in order to further expand the knowledge of plant extract composition. Most works in this field employ HPLC, when exploring non-structural and cell wall carbohydrates from Rhynchelytrum repens. Phenolic compounds were studied by classical chromatography techniques and UV-vis spectrophotometry, with C-glycosylated flavonoids being detected but with no further details regarding the chemical structure of these compounds. In this work we employ paper spray ionization mass spectrometry (PS-MS) for the evaluation of the chemical profile of R. repens methanol extract. Positive ionization mode identified 15 compounds, belonging to flavonoids, fatty acids, and other classes of compounds; negative mode ionization was able to identify 20 compounds comprising the classes of quinic acids, stilbenes and flavonoids. PS-MS proved effective for the evaluation of R. repens extracts, making it possible to identify a total of thirty-five compounds. The bioactive properties attributed to R. repens were confirmed by the identification and characterization of compounds identified by PS-MS.
“…The protocol for obtaining fractions from sugarcane juice was carried out according our previous work (Gomes et al, 2020). The juice from different varieties of S. officinarum (150 mL) was autoclaved (1 atm, 121 ºC) for 20 min to eliminate contaminating microorganisms.…”
Section: Preparation Of Methanol Fraction After Juice Fermentationmentioning
This work aimed to evaluate the antinociceptive activity of methanolic fractions from sugarcane varieties (MFSCf) in classic nociception models, as well as evaluate the interactions between bioactive compounds and the µ-opioid receptor (µOR) through docking analysis. Five methanolic fractions of sugarcane juice were obtained and analysed by LC-ESI-MS/MS. The fractions and standards of phenolic compounds were evaluated in a nociception model using the formalin test. All MFSCfs exhibited antinociceptive activity in the first phase of the formalin test. Docking analyses corroborates with the in vivo test results, suggesting that the phenolic substances are able to activate µOR. These results, for the first time, implicate phenolic constituents from sugarcane juice and other phenolic compounds in the activation of µOR. The antinociceptive activity of fractions from sugarcane juice suggests the potential pharmacological use of this species, widely cultivated in Brazil
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