Antinociceptive and antiedematogenic effects of the aqueous extract of Hyptis pectinata leaves in experimental animals

119

ABSTRACT:The infl ammatory reaction is a response of the organism against an injury and it involves the action of complex events and mediators through of the blood vessels. The present work is a literature survey of the extracts of plants with anti-infl ammatory activity studied in Brazil. The review refers to 75 plants with their families, parts used, type of extract used, bioassay models and their activity.

Section: Anti-infl Ammatory Activity Of Crude Plant Extractsmentioning

confidence: 96%

Review of the plants with anti-inflammatory activity studied in Brazil

Falcão¹,

Lima²,

Santos³

et al. 2005

119

“…Recent studies have shown that species such as Protium heptaphyllum (Aubl.) Marchand, P. kleinii Cuatrec., Burseraceae, Hyptis pectinata (L.) Poit., Lamiaceae and Zataria multiflora Boiss., Lamiaceae, rich in p-cymene, show antinociceptive activity in rodents (Oliveira et al, 2005;Otuki et al, 2001;Bispo et al, 2001;Ramezani et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

p-Cymene reduces orofacial nociceptive response in mice

Santana¹,

Quintans‐Júnior²,

Cavalcanti³

et al. 2011

This study investigated the possible antinociceptive effect of p-cymene in different tests of orofacial nociception. The animals (mice) were pretreated (i.p.) with p-cymene (25, 50, 100 mg/kg), morphine (5 mg/kg), or vehicle (0.2% Tween 80+saline), and were then subsequently administered, subcutaneously into their upper lip: formalin, capsaicin, and glutamate. The nociceptive behavior response was characterized by the time in s that the mice remained rubbing the orofacial region, for a period of 40 min in the formalin test (first phase, 0-6 min; and second phase, 21-40 min), and for 42 and 15 min in the capsaicin and glutamate tests, respectively. To verify the possible opioid involvement in the antinociceptive effects, naloxone (i.p.) was administered into the mice 15 min prior to the pretreatment with p-cymene (100 mg/kg). Finally, whether or not the p-cymene evoked any change in motor performance in the Rota-rod test was evaluated. The results showed that the treatment with p-cymene, at all doses, reduced (p<0.001) the nociceptive behavior in all nociception tests. The antinociceptive effect of p-cymene was antagonized by naloxone (1.5 mg/kg). Additionally, mice treated with p-cymene did not show any change in motor performance. In conclusion, p-cymene attenuated orofacial nociception, suggesting an involvement of the opioid system in this effect. Thus, p-cymene might represent an important biomolecule for management and/or treatment of orofacial pain.

“…Various species are used in folk medicine as anti-inflammatory, antinociceptive, anticonvulsant, and antiulcerogenic (Barbosa & Ramos, 1992;Akah & Nwambie, 1993;Kuhnt et al, 1995;Bispo et al, 2001). The aqueous extract and volatile oil of Hyptis pectinata showed antiedematogenic and antinociceptive activities in animal models (Bispo et al, 2001;Arrigoni-Blank et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Preliminary study on the anti-inflammatory and antioxidant activities of the leave extract of Hyptis fruticosa Salzm. ex Benth., Lamiaceae

Andrade¹,

Oliveira²,

Santos³

et al. 2010

RESUMO: "Avaliação das atividades anti-inflamatória e antioxidante do extrato etanólico das folhas da Hyptis fruticosa Salzm. ex Benth., Lamiaceae". Várias espécies do gênero Hyptis são utilizadas na medicina popular para tratar processos inflamatórios. Para avaliar as ações anti-inflamatória e antioxidante da Hyptis fruticosa Salzm. ex Benth., Lamiaceae, utilizou-se extrato etanólico (EE) das folhas desta planta e suas partições n-C 6 H 14 , CHCl 3 , AcOEt e MeOH/H 2 O. O tratamento oral com o EE das folhas da H. fruticosa (100, 200 e 400 mg/kg) ou suas partições n-C 6 H 14 , AcOEt e MeOH/H 2 O (50, 100 e 200 mg/kg) apresentou atividade inibitória sobre a formação de edema e migração leucocitária para a cavidade peritoneal induzidas pela carragenina em ratos (p<0,05). Entretanto, a partição CHCl 3 não causou nenhum efeito sobre a formação de edema e migração de células peritoneais. O EE bruto e a partição AcOEt apresentaram alto potencial antioxidante (IC50 = 35,00±1,01 e 36,67±2,65 μg/mL DPPH, respectivamente), similar ao composto referência (IC50 = 16,67±1,21 μg/mL). Em conclusão, demonstrou-se que a H. fruticosa apresenta atividades anti-inflamatória e antioxidante.Unitermos: Anti-inflamatória, antioxidante, edema, Hyptis fruticosa, Lamiaceae, migração celular.ABSTRACT: Various species of Hyptis are used in folk medicine as anti-inflammatory. In order to evaluate the actions of Hyptis fruticosa Salzm. ex Benth., Lamiaceae, studies were performed on anti-inflammatory and antioxidant activities. The ethanol extract (EE) of H. fruticosa leaves and its n-C 6 H 14 , CHCl 3 , EtOAc, and MeOH/H 2 O partitions were used in the following experiments. Oral treatment with the EE of H. fruticosa leaves (100, 200, and 400 mg/kg) or its n-C 6 H 14 , EtOAc, and MeOH/H 2 O partitions (50, 100, and 200 mg/kg) elicited inhibitory activity (p<0.05) on carrageenan-induced oedema formation and leukocyte migration into the peritoneal cavity in rats. However, the CHCl 3 partition did not show any inhibitory effect on paw oedema and peritonitis experimental models. The EE and EtOAc partition present the highest antioxidant potential (IC50 = 35.00±1.01 and 36.67±2.65 μg/ mL DPPH, respectively), similar to the reference compound (IC50 = 16.67±1.21 μg/mL). In conclusion, H. fruticosa shows anti-inflammatory and antioxidant activities.