Antinociceptive and Antiedematogenic Activities of Andrographolide Isolated From Andrographis paniculata in Animal Models

Abstract

The current study was performed to evaluate the antinociceptive and antiedematogenic properties of andrographolide isolated from the leaves of Andrographis paniculata using two animal models. Antinociceptive activity was evaluated using the acetic acid- induced writhing and the hot-plate tests, while antiedematogenic activity was measured using the carrageenan-induced paw edema test. Subcutaneous (s.c.) administration of andrographolide (10, 25, and 50 mg/kg) did not affect the motor coordination of the experimental animals but produced significant (p < .05) antinociceptive activity when assessed using both tests. However, 2 mg/kg naloxone failed to affect the 25 mg/kg andrographolide activity in both tests, indicating that the activity was modulated via nonopioid mechanisms. Furthermore, andrographolide showed significant (p < .05) antiedematogenic activity. In conclusion, the results obtained suggest that andrographolide has antinociceptive and antiedematogenic activities; it may be useful for treating pain and inflammation once human studies are conducted.

Neuroprotective effects of andrographolide in a rat model of permanent cerebral ischaemia

“…Andrographolide is the major bioactive compound isolated from Andrographis paniculata (Chuan‐Xin‐Lian in Chinese), a medicinal herb that is widely used in China and other parts of Asia for the treatment of upper respiratory tract infections (Roxas and Jurenka, 2007). We now know that andrographolide exerts a wide range of therapeutic actions, including immunosuppressant (Burgos et al ., 2005; Iruretagoyena et al ., 2006), anti‐thrombotic (Li et al ., 2009), anti‐inflammatory (Wang et al ., 2007b; Abu‐Ghefreh et al ., 2009; Bao et al ., 2009), anti‐neoplastic (Varma et al ., 2009), anti‐viral (Lin et al ., 2008; Chen et al ., 2009), anti‐bacterial (Jiang et al ., 2009), anti‐diabetic (Zhang et al ., 2009b), antioxidative stress (Akowuah et al ., 2009), anti‐pyretic (Suebsasana et al ., 2009) and anti‐oedematogenic and anti‐nociceptive (Lin et al ., 2009; Sulaiman et al ., 2010) activities. Several of these pharmacological properties would appear to be beneficial in cerebral ischaemia.…”

Enhancing bioavailability and hepatoprotective activity of andrographolide fromAndrographis paniculata, a well-known medicinal food, through its herbosome

“…It has been shown to exert potent anticancer activity against human colorectal carcinoma Lovo cells by inhibiting cell cycle progression and also reducing MMP‐7‐mediated cellular events 23. Andrographolide was also found to possess significant antinociceptive and antiedematogenic activities via nonopioid mechanisms 24. Further, 14‐α‐lipoyl andrographolide derived from angrographolide was found to have significant activity against influenza A viruses, including the H5N1 avian influenza virus 25…”

Anti-inflammatory effect of novel andrographolide derivatives through inhibition of NO and PGE2 production

“…f) Nees, which is extensively used in China, India, and other Asian countries for the treatment of viral infections and inflammatory diseases [1][2][3]. Studies have demonstrated that andrographolide significantly inhibits rat paw edema induced by carrageenin, kaolin, and nystatin, inhibits granuloma formation induced by cotton pellet, and inhibits the increase in vascular permeability induced by acetic acid [4][5][6][7]. Activation of the expression of inducible isoforms of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) leads to the production of excessive amounts of nitric oxide (NO) and prostaglandin E 2 (PGE 2 ), which play key roles in the inflammation process [8].…”