Antinociceptive activities of Artocarpus lacucha Buch-ham (Moraceae) and its isolated phenolic compound, catechin, in mice

Abstract

Background The present study evaluated the antinociceptive effect of the bark of Artocarpus lacucha , which is used for the treatment of stomachache, headache and boils in the traditional system of medicine. Methods The antinociceptive activity was investigated by the tail immersion, hot plate, acetic acid- & formalin-induced nociception and carrageenan-induced paw edema tests using a hydro-methanolic extract of A. lacucha bark. The plant extract was found to contain a substantial amount of phenolic compounds according to the total phenolic and flavonoid content assay. A phenolic metabolite, (+)-catechin, has been isolated using different chromatographic techniques. The compound was characterized with 1D and 2D NMR spectroscopic data. (+)-catechin, isolated from A. lacucha was assessed for antinociceptive effects swiss albino mice. Furthermore, the possible involvement of opioid receptors and ATP-sensitive K + channel for the effect of the plant extract and (+)-catechin has been justified using naloxone and glibenclamide, respectively. Results Oral administration (p.o) of the plant extract (50–200 mg/Kg b.w.) resulted in significant thermal pain protection in the hot plate and tail immersion tests. The action of the plant extract was significantly antagonized by naloxone, a non-selective opioid antagonist, in the hot plate and tail immersion tests, which supports the involvement of opioid receptors. Both the plant extract and (+)-catechin, (50–200 mg/Kg b.w., p.o.) significantly diminished the acetic acid- & formalin-induced nociception, and carrageenan-induced paw edema. Glibenclamide, an ATP-sensitive K + channel blocker, significantly reversed their effect in the acetic acid-induced writhing test which indicates the participation of ATP-sensitive K + channel system. Conclusions The investigation revealed potential central and peripheral antinociceptive effects of A. lacucha bark supports its applications in the traditional system of medicine. Electronic supplementary material The online version of this article (10.1186/s12906-019-2565-x) contains supplementary material, which is available to authorized users.

An Investigation of the Molecular Mechanisms Underlying the Analgesic Effect of Jakyak‐Gamcho Decoction: A Network Pharmacology Study

“…e analgesic activity of the chemical components of JGd has been previously reported. (+)-Catechin and pinocembrin produce analgesic, antineuropathy, and antinociceptive effects [295][296][297]. Albiflorin might play a pharmacological role as an analgesic, antineuropathy, and antinociceptive compound that can reduce pain intensity via the functional modulation of calcium channels, mitogen-activated protein kinase (MAPK) pathways, and various cytokines and chemokines [127,298].…”

Evaluación farmacológica de la actividad antinociceptiva y análisis fitoquímico de los extractos activos de Salvia purpurea Cav.

“…De dichos compuestos ya se han demostrado sus propiedades analgésicas reiterando con este estudio su participación como bioactivos. La apigenina, catequina y naringenina presentaron un potente efecto antinociceptivo y antiinflamatorio en las diversas pruebas donde fueron evaluadas (placa caliente, inmersión de la cola, "writhing", formalina y carragenina) (Pinheiro et al 2012, Islam et al 2019, Xue et al 2019. La florizina y su aglicona floretina son reportadas principalmente por sus efectos antinflamatorios (Sekhon-Loodu et al 2015, Kim et al 2018.…”

Methanol Extract of Dicranopteris linearis Leaves Attenuate Pain via the Modulation of Opioid/NO-Mediated Pathway

“…As part of an attempt to establish the antinociceptive potential of D. linearis and to promote the use of medicinal plants as pain-relieving agent, MEDL was also subjected to phytoconstituents analyses using the UHPLC-ESI-HRMS and GC-MS methods to determine the presence of polyphenolics or any volatile bioactive compounds with potential antinociceptive activity, respectively. The UHPLC-ESI-HRMS analysis of MEDL leads to identification of approximately 30 polyphenolic compounds of which several of them, such as gallic acid [60,61], ferulic acid [62], protocatechuic acid [63], caffeic acid [64,65], p-coumaric acid [66], rutin [67,68], isoquercitrin [69], astragalin [70], catechin [71], quercetin [72,73], apigenin [74] and kaempferol [75], have been reported to show antinociceptive activity. These reports also revealed that: (i) gallic acid was reported to show low antinociceptive activity against the acetic acid-induced nociception [60] while its derivative (gallic acid ethyl ester) was reported to attenuate bradykininand formalin-induced nociception [61].…”

“…These reports also revealed that: (i) gallic acid was reported to show low antinociceptive activity against the acetic acid-induced nociception [60] while its derivative (gallic acid ethyl ester) was reported to attenuate bradykininand formalin-induced nociception [61]. In addition, gallic acid ethyl ester was ineffective in the hot-plate test and demonstrated partly the opioid/NO-independent action; (ii) ferulic acid exerts an opioid-mediated antinociceptive activity when assessed using the hot plate test [62]; (iii) protocatechuic acid also exerts an opioid-mediated antinociceptive activity when assessed using the hot plate test [63]; (iv) caffeic acid demonstrated the antinociceptive activity against the abdominal constriction test and the late phase of the formalin-induced nociception, but not the hot plate test [64] whereas the dodecyl ester derivative of caffeic acid were reported to produce antinociceptive activity against the abdominal constriction test as well as the formalin-, capsaicin-and glutamate-induced nociceptive model [65]; (v) rutin exerts antinociceptive activity when assessed using the abdominal constriction test and the formalin-induced nociception, respectively [66,67] with Hernandez-Leon et al [67] also showed that rutin produces an opioid-mediated antinociceptive activity only in the late phase of the formalin-induced test; (vi) isoquercitrin exhibits antinociceptive activity against the abdominal constriction and formalin tests [68]; (vii) astragalin demonstrates an opioid-mediated antinociceptive activity when assessed using the hot plate test and the formalin-induced nociception [69]; (viii) catechin produces antinociceptive activity against the abdominal constriction, hot plate and formalin-induced paw licking tests with an opioid-independent activity shown using the hot plate test [70]; (ix) quercetin was earlier reported to show an opioid-mediated antinociceptive activity when assessed using the hot plate test [71] while later study demonstrates that quercetin produces antinociceptive activity against the abdominal constriction test, as well as the formalin-, capsaicin-and glutamate-induced nociceptive tests that involves an interaction with l-arginine/NO pathway [72]; (x) apigenin was found to show antinociceptive activity against the abdominal constriction, hot plate and formalin-induced paw licking tests with the centrally-mediated opioid activity proven using the hot plate test [73]; and (xi) kaempferol derivatives (i.e., kaempferol 3-O-rutinoside, kaempferol 3-O-glucoside and kaempferol-3,7-di-O-α-l-rhamnopyranoside) was reported to exert antinociceptive activity against the abdominal constriction and formalin-induced paw licking tests [74,75]. In addition, Ali et al [75] also...…”