Antineoplastic effects of Chlorella pyrenoidosa in the breast cancer model

Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

“…The criteria for categorization (solidization, cell atypia, mitotic activity index and necrosis) were chosen according to the standard diagnostic method of classification. HG carcinomas were considered to be tumours with ≥2 positive criteria; LG carcinomas were tumours with ≤1 positive criterion . Serum metabolic parameters (total cholesterol, very low‐density lipoprotein cholesterol, low‐density lipoprotein cholesterol, high‐density lipoprotein cholesterol, triacylglycerols, glucose) were evaluated using an Olympus AU640 (Olympus Optical, Tokyo, Japan) automatic biochemical analyser.…”

“…The detection of selected markers of mechanism of action (mentioned below) was carried out by indirect immunohistochemical method on whole paraffin sections, utilizing commercially available rat‐specific antibodies (Santa Cruz Biotechnology, Paso Robles, CA, USA; Dako, Glostrup, Denmark; Bioss, Woburn, MA, USA; GeneTex, Irvine, CA, USA; Abcam, Cambridge, MA, USA; Boster Biological Technology, Pleasanton, CA, USA; Thermo Fisher Scientific, Rockford, IL, USA). All steps of the immunohistochemical staining were processed according to the manufacturers’ recommendations as we described previously . The concentration used for each primary antibody is as follows: caspase‐3 1:500, Bax 1:200, Bcl‐2 1:200, Ki‐67 1:50, VEGFA 1:150, VEGFR‐2 1:80, MDA 1:1000, CD24 1:200, CD44 1:200, ALDH1A1 1:500, H3K4m3 1:500, H3K9m3 1:400, H4K20m3 1:300, H4K16ac 1:200.…”

“…For flow cytometric analysis (FCM), floating and adherent cells were harvested together 24, 48 and 72 hrs after treatment (CLO final concentrations 350 and 450 μg/ml), washed in PBS, resuspended in PBS and stained prior to analysis as described previously . Fluorescence was detected, after 15‐ to 30‐min.…”

Effects of anastrozole on Ki-67 antigen expression in the vaginal epithelium of female rats in persistent estrus

“…Gerdes et al (23) found a Ki-67 antibody that recognized a nuclear antigen present in proliferating cells and absent in resting cells, making it ideal for the evaluation of cell proliferative activity. Anastrozole is administered in postmenopausal women with hormonesensitive breast cancer; however, it has been shown that aromatase inhibitors may lead to adverse effects in premenopausal female rats, such as dietary exemestane-induced mammary carcinogenesis (24). Nevertheless, the effects of anastrozole administered by oral gavage to premenopausal rats, as opposed to oral exposure in the diet, are unknown.…”

Metabolic and motor activity effects of microalgae (Chlorella vulgaris) in laboratory mice

“…6). Несмотря на многочисленные исследования, в которых были показаны антиоксидантные и противовос-палительные свойства хлореллы, противоопухолевые или другие положительные эффекты на организмы животных или человека (Vijayavel et al, 2007;Wang et al, 2010;Panahi et al, 2012aPanahi et al, , b, 2013Kim et al, 2015;Kubatka et al, 2015), работы по влиянию микроводорослей на поведение практически отсутствуют. Тем не менее при исследовании эффектов хлореллы, которую использовали как дополне-ние к стандартной схеме терапии антидепрессантами па-циентов с диагнозом Большое депрессивное расстройство (БДР), характеризующееся в том числе утратой интересов, снижением энергичности и повышенной утомляемостью, была показана ее эффективность (Panahi et al, 2015).…”