Antineoplastic Effects of Bee Honey andNigella sativaon Hepatocellular Carcinoma Cells

Abstract

Objectives: To evaluate in vitro antitumor effects of bee honey (BH) and Nigella sativa (NS) on HepG2 through their antioxidant and apoptotic activities. Methods: HepG2 cell line was treated with different concentrations of diluted unfractionated BH and different concentrations of alcohol extract of NS. Exposure lasted for different time durations (6-72 hours), both dose-response and time course-response were conducted. Cell viability was tested by trypan blue exclusion test. Total antioxidant status and caspase-3 activity were estimated in the cell lysate. Nitric oxide levels were measured in culture supernatants of both treated and untreated HepG2 at all indicated times. Results: Treatment of HepG2 cells with BH and NS leads to a significant decrease in both the number of viable HepG2 cells and the levels of nitric oxide on one hand, but improvement of the total antioxidant status and caspase-3 activity on the other, especially in HepG2 cells treated with higher doses of BH and NS (20% and 5000 μg/mL, respectively) and for longer duration (72 hours). Conclusions: BH and NS are effective in reducing the viability of HepG2 cells, improving their antioxidant status and inducing their apoptotic death.

The Effect of Tualang Honey on Human Periodontal Ligament Fibroblast Proliferation and Alkaline Phosphatase Level

“…The observed biological activities of honey may be due to its chemical constituents and high sugar content (Hassan et al 2010). Most literature reported on the effect of honey treatment on external wounds rather than lesions and infections of mucosal surfaces, but the features observed are relevant also to many aspects of dental practice, such as removing FIGURE 1.…”

“…It is acidic in nature (pH4) and consists of 80% sugars (fructose, glucose, sucrose, and maltose), 17-20% water and 4% of other substances (pollen grains, enzymes, hydrogen peroxide, amino acids, organic acids, polyphenols, vitamins and minerals). Though the exact biochemical mechanism remains unclear, studies have shown that honey has anti-inflammatory, anti-oxidant, apoptotic and immunomodulatory properties (Hassan et al 2010). In addition, honey accelerates wound healing by forming new tissues and also has antimicrobial properties (Kannan et al 2009).…”

Honey as a Potential Natural Anticancer Agent: A Review of Its Mechanisms

“…The exact antioxidant mechanism is unknown, but the proposed mechanism is through hydrogen donation, free radical sequestration, metallic ion chelation, flavonoids substrates for hydroxyl and superoxide radical actions [71]. The antioxidant capacity of honey contributes to the prevention of several acute and chronic disorders such as diabetes [72], inflammatory disorders [73], cardiovascular diseases [74], and cancer [75, 76]. The phenolic acids and flavonoids are responsible for the well-established antioxidant activity of honey [77].…”

UJI SITOTOKSISITAS DAN ANTIPROLIFERATIF FRAKSI ETIL ASETAT EKSTRAK ETANOL BIJI JINTEN HITAM (Nigella sativa, Lour) TERHADAP SEL MIELOMA

“…Nigella sativa dilaporkan mempunyai efek antioksidan (Burits and Bucar, 2000), penghambatan lipooksigenase (Dakhakhny et al, 2002), antiinflamasi, antikanker (Tariq, 2008), analgesik (Randhawa, 2008), sitotoksik terhadap hepatoma HepG2 cell line (Thabrew et al, 2005), hepatocelluler carcinoma cells (Hassan et al, 2008) Berdasarkan data-data yang tersedia tersebut, maka dapat dikatakan bahwa tanaman Nigella Sativa, Lour memiliki potensial untuk dikembangkan sebagai agen antikanker. Penelitian ini bertujuan untuk meneliti efek sitotoksik dan antiproliferatif ekstrak etanol biji jinten hitam terhadap sel mieloma.…”