The Effect of Tualang Honey on Human Periodontal Ligament Fibroblast Proliferation and Alkaline Phosphatase Level

Lau, Xin Yu; Taib, Haslina; Berahim, Zurairah; Ahmad, A.L.; Zainuddin, Siti Lailatul Akmar

doi:10.17576/jsm-2015-4407-14

Cited by 5 publications

(2 citation statements)

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“…While osteoblast and fibroblast cell migration were represented by the length of the wound, the registered values of wound length were (324.7, 336.4, 547) and (308.3, 464.2, 1032) respectively in 48 h (Tables 7 , 11 ). This aligns with previous research showing that Tualang honey stimulates fibroblast cell proliferation at low concentrations (0.02%) but inhibits it at high concentrations [ 57 ]. Additionally, low concentrations of Pasture honey and Manuka honey have been found to activate phagocytes and stimulate lymphocyte proliferation which promotes wound healing [ 58 ].…”

Section: Discussionsupporting

confidence: 92%

Combination of stem cell-derived secretome from human exfoliated deciduous teeth with Yemeni Sidr honey on cell viability and migration: an in vitro study

Al-Hadi

2024

BDJ Open

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Introduction Bone diseases have a profound global impact, especially when the body’s innate regenerative capacity falls short in the face of extensive damage. Stem cells from human exfoliated deciduous teeth (SHEDs), discovered in 2003, offer a promising solution for tissue repair, as they self-renew naturally and are easily obtainable. Mesenchymal stem cells (MSCs), including SHEDs, are believed to promote tissue regeneration by releasing growth factors, collectively known as the secretome. Aims This study explored the potential of combining SHED-derived secretome with Yemeni Sidr honey to improve osteoblast and fibroblast cell viability and migration. Materials and methods The experiment involved treating cell cultures of two types of rat cell lines - 7F2 osteoblast and BHK-21 fibroblast immortalized cells - with SHED-derived secretome and Yemeni Sidr honey. After the treatment, cell viability was measured using the MTT assay, which calculates OD at 590 nm. Additionally, the scratch assay was conducted to evaluate cell migration, and ImageJ software was used for data processing. Results The findings indicated that combining SHED-derived secretome and Yemeni Sidr honey enhanced osteoblast and fibroblast cell viability and migration. Furthermore, the study highlighted the difference in the stimulative potential of SHED-derived secretome, Yemeni Sidr honey, and their combination, on the viability and migration of the cultured cells. Conclusion The research concludes that combining SHED-derived secretome with Yemeni Sidr honey has the potential to promote cell viability and migration in in-vitro settings. The synergistic application of these substances has been found to be more effective -when combined in a dose-dependent manner- than their counterparts. Overall, the current study serves as a foundation for further investigations to establish if the explored substance has any useful clinical applications.

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Section: Discussionsupporting

confidence: 92%

Combination of stem cell-derived secretome from human exfoliated deciduous teeth with Yemeni Sidr honey on cell viability and migration: an in vitro study

Al-Hadi

2024

BDJ Open

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“…The finding showed that 0.02% Tualang honey concentration stimulated a higher proliferation rate than the control. However, at a higher concentration of Tualang honey (5%), the cells became rounded and floating, indicating that a higher honey concentration could inhibit cell proliferation [ 98 ].…”

Section: Cell Migration and Proliferation On Honey-based Wound Dressingsmentioning

confidence: 99%

Polymer-Based Hydrogel Loaded with Honey in Drug Delivery System for Wound Healing Applications

Yasin,

Said,

Halib

et al. 2023

Polymers

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Excellent wound dressings should have crucial components, including high porosity, non-toxicity, high water absorption, and the ability to retain a humid environment in the wound area and facilitate wound healing. Unfortunately, current wound dressings hamper the healing process, with poor antibacterial, anti-inflammatory, and antioxidant activity, frequent dressing changes, low biodegradability, and poor mechanical properties. Hydrogels are crosslinked polymer chains with three-dimensional (3D) networks that have been applicable as wound dressings. They could retain a humid environment on the wound site, provide a protective barrier against pathogenic infections, and provide pain relief. Hydrogel can be obtained from natural, synthetic, or hybrid polymers. Honey is a natural substance that has demonstrated several therapeutic efficacies, including anti-inflammatory, antibacterial, and antioxidant activity, which makes it beneficial for wound treatment. Honey-based hydrogel wound dressings demonstrated excellent characteristics, including good biodegradability and biocompatibility, stimulated cell proliferation and reepithelization, inhibited bacterial growth, and accelerated wound healing. This review aimed to demonstrate the potential of honey-based hydrogel in wound healing applications and complement the studies accessible regarding implementing honey-based hydrogel dressing for wound healing.

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Combinatorial effects of hydroxyapatite and Tualang honey on medication-related osteonecrosis of the jaw (MRONJ): An in vitro study

Fauzi,

Sabri,

Halim

et al. 2024

Journal of Stomatology, Oral and Maxillofacial Surgery

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The Effect of Tualang Honey on Human Periodontal Ligament Fibroblast Proliferation and Alkaline Phosphatase Level

Abstract: Previous studies on honey have shown its potential as an alternative approach in optimizing medical resources for periodontal treatment. However, to date, there has been no study published on the effects of honey on human periodontal ligament fibroblasts (HPDLFs

Cited by 5 publications

References 10 publications

Combination of stem cell-derived secretome from human exfoliated deciduous teeth with Yemeni Sidr honey on cell viability and migration: an in vitro study

Combination of stem cell-derived secretome from human exfoliated deciduous teeth with Yemeni Sidr honey on cell viability and migration: an in vitro study

Polymer-Based Hydrogel Loaded with Honey in Drug Delivery System for Wound Healing Applications

Combinatorial effects of hydroxyapatite and Tualang honey on medication-related osteonecrosis of the jaw (MRONJ): An in vitro study

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