Antinematodal Activity of Some Tropical Rainforest Plants against the Pinewood Nematode, Bursaphelenchus xylophilus

Alen, Yohannes; Nakajima, Shûhei; Nitoda, Teruhiko; Baba, Naomichi; Kanzaki, Hiroshi; Kawazu, Kazuyoshi

doi:10.1515/znc-2000-3-425

Cited by 30 publications

(18 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detailed literature review indicated that various classes of phytochemicals have been identified in different parts of M. malabathricum such as fatty acids and aliphatic constituents (Dinda & Saha 1986a, Dinda & Saha 1986b, Dass & Kotoky 1988, sterols (Manzoor-I-Khuda et al 1981), phenolic acids (Lowry 1968), flavonoids and their glycosides (Dinda & Saha 1988, Mohandoss & Ravindran 1993, Wong et al 2012, tannins (Yoshida et al 1992a(Yoshida et al , 1992b, anthocyanins (Lowry 1976), amino acids (Dinda & Saha 1985) and so on. Different parts of the plant have been shown to exert diverse biological activities like antibacterial and antifungal (Grosvenor et al 1995, Wiart et al 2004, Johnny et al 2010, Maji et al 2010), cytotoxic (Susanti et al 2007 and antiviral (Nazlina et al 2008, Lohezic-Le Devehat et al 2002, antiparasitic (Alen et al 2000), antioxidant (Susanti et al 2007, anticoagulant (Manicam et al 2010), platelet-activating factor inhibitory (Jantan et al 2005), wound healing (Sunilson et al 2008), anti-ulcer (Hussain et al 2008), anti-diarrheal (Sunilson et al 2009), anti-nociceptive (Sulaiman et al 2004), and anti-pyretic (Zakaria et al 2006) at different concentrations.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis and Constituents of Hexane Extract of Melastoma Malabathricum L

Giri

Rajbhandari

2018

J. Inst. Sci. Tech.

View full text Add to dashboard Cite

Hexane, dichloromethane, ethyl acetate, methanol and 50 % aqueous methanol extracts of leaf, flower and fruit of Melastoma malabathricum were screened for the presence of different classes of phytochemicals. In phytochemical screening, ethyl acetate, methanol and 50 % aqueous methanol extracts showed the presence of phenolics and flavonoids. Glycosides were found only in the 50 % methanol extract of fruit. Free radical scavenging activity of methanol extract of leaf, flower and fruit was determined using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical. Total phenolic content was measured spectrophotometrically using Folin-Ciocalteu, total flavonoids content by using aluminum chloride and total sugar content by using anthrone reagent. Gallic acid was used as a standard for the calibration of phenolics, catechin for flavonoids and glucose for carbohydrates. The highest amount of phenolic was found in the 50 % methanol extract of leaf (242.88 ± 3.52 mg GAE/g extract), the highest amount of flavonoids was found in the ethyl acetate extract of leaf (127.41 ± 6.33 mg CE/g extract). Total sugar in 50 % methanol extract of fruit was found to be 151.12 ± 8.79 mg GE/g extract. Methanol extracts of flower showed the strongest antioxidant activity with IC50 value of 17.23 μg/ml. Silyl derivative of hexane extracts were prepared by using N-methyl-N-(trimetylsilyl) trifluoroacetamide for gas chromatography-mass spectroscopic (GC-MS) analysis. The GC-MS analysis of hexane extract allowed the identification of 17 phyto-constituents by comparing the fragmentation pattern of each component in mass spectrum with the standard NIST mass spectral database.

show abstract

Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis and Constituents of Hexane Extract of Melastoma Malabathricum L

Giri

Rajbhandari

2018

J. Inst. Sci. Tech.

View full text Add to dashboard Cite

show abstract

“…Essential oils have long been known to have significant biological activities. Recent studies have shown that some essential oils appear to have good nematicidal activity against the PWN under laboratory conditions [ 5 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Bioassays Against Pinewood Nematode: Assessment of a Suitable Dilution Agent and Screening for Bioactive Essential Oils

et al. 2012

View full text Add to dashboard Cite

Acetone was investigated and found to be an appropriate alternative to Triton X-100 as a solvent of essential oils in bioassays aimed to investigate their effects on pinewood nematode (Bursaphelenchus xylophilus) mortality. Therefore it was used as dilution agent to screen the effectiveness of fifty two essential oils against this pest. Thirteen essential oils were highly effective, resulting in more than 90% pinewood nematode mortality at 2 mg/mL, with six of them resulting in 100% mortality. LC100 values ranged between 0.50 mg/mL and 0.83 mg/mL for the essential oils of Origanum vulgare and Satureja montana, respectively. Essential oils were submitted to gas chromatography and gas chromatography-mass spectrometry analysis and their chemical composition established. Data from essential oils with 100% mortality at 2 mg/mL and other essential oils previously found to have LC100 ≤ 2 mg/mL was combined, their chemical profiles investigated by correspondences analysis plus automatic classification.

show abstract

“…By using this method, methanolic extracts of 63 tropical rainforest plants were assessed and 27 plants were proved to contain active component(s) against the phytopathogenic nematode, Bursaphelenchus xylophilus. Among them, the extract of the sap of Knema hookeriana was shown to have pronounced activity (Alen et al, 2000). A literature survey revealed that the isolation work of antinematodal compounds from this species has not been reported so far.…”

Section: Introductionmentioning

confidence: 99%

Two Antinematodal Phenolics from Knema hookeriana, a Sumatran Rainforest Plant

Alen

Nakajima

Nitoda

et al. 2000

Zeitschrift Für Naturforschung C

Self Cite

View full text Add to dashboard Cite

The activity-guided chromatographic purification of the methanol extract of Knema hookeriana, using pine wood nematodes Bursaphelenchus xylophilus has successfully led to the isolation and characterization of two phenolic antinematodal compounds with minimun effective dose (MED) of 4.5 and 20 μg/cotton ball (μg/ bl.) or 0.018 and 0.073 μᴍ/cotton ball (μᴍ/bl.), respectively. Based on their chemical and spectral properties, these compounds were determined to be 3-undecylphenol (1) and 3-(8Z-tridecenyl)-phenol (2). These compounds were isolated for the first time from this species, and 2 seems to be a novel compound.

show abstract

Antinematodal Activity of Some Tropical Rainforest Plants against the Pinewood Nematode, Bursaphelenchus xylophilus

Cited by 30 publications

References 8 publications

Phytochemical Analysis and Constituents of Hexane Extract of Melastoma Malabathricum L

Phytochemical Analysis and Constituents of Hexane Extract of Melastoma Malabathricum L

Bioassays Against Pinewood Nematode: Assessment of a Suitable Dilution Agent and Screening for Bioactive Essential Oils

Two Antinematodal Phenolics from Knema hookeriana, a Sumatran Rainforest Plant

Contact Info

Product

Resources

About