Antimycobacterial, antiplasmodial and toxicological effect of oleanolic acid and its derivative from Syzygium aromaticum Linn (Myrtaceae)

Fadipe, V.O.; Opoku, Andy R.; Shintre, Suhas A.; Singh, Parminder; Singh, Moganavelli; Mongalo, N.I.

doi:10.1016/j.sajb.2016.02.043

Cited by 4 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Melting point (mp): Melting points of the compounds were determined on a Stuart Scientific SMP3 apparatus. The currently isolated compound (1) was isolated and identified as β-Sitosterol (NMR data not shown), while the other three compounds (2, 3 and 4) were identified earlier in our research group [ 23 ]. …”

Section: Methodsmentioning

confidence: 94%

Isolation of anti-mycobacterial compounds from Curtisia dentata (Burm.f.) C.A.Sm (Curtisiaceae)

Fadipe

Mongalo

Opoku

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundTuberculosis is counted amongst the most infectious and lethal illnesses worldwide and remains one of the major threats to human health. The aim of the current study was to isolate and characterize anti-mycobacterial compounds present in Curtisia dentata (Burm.f.) C.A.Sm, a medicinal plant reportedly used in the treatment of tuberculosis, stomach ailments and sexually transmitted infections.MethodsThe bioassay guided principle was followed to isolate the anti-mycobacterial compounds. The crude ethanol extracts of the leaves was partitioned with various solvents four compounds such as β-sitosterol, betulinic acid, ursolic acid and lupeol were successfully isolated. The compounds and their derivatives were evaluated for anti-mycobacterial activity using Microplate Alamar Blue Assay (MABA) against Mycobacterium tuberculosis H37RV (ATCC 27294). Furthermore, the derivatives were investigated for their toxicity against HepG2 and HEK293 using the MTT assay.ResultsThe methanol fraction had the lowest minimum inhibitory concentration (MIC) of 22.2 μg/ml against the selected Mycobacterium strain when compared to other fractions. Ursolic acid acetate (UAA) was the most active compound with MIC value of 3.4 μg/ml. The derivatives had varying degrees of toxicity, but were generally non-toxic to the selected cell lines. Derivatives also exhibited highest selectivity index and offers a higher safety margin.ConclusionsThe derivatives had better antimicrobial activity and low cytotoxic effects compared to isolated compounds. These increased their selectivity. It appears that acetylation of both betulinic acid and ursolic acid increased their activity against the selected Mycobacterium species. The results obtained in this study gives a clear indication that Curtisia dentata may serve as major source of new alternative medicines that may be used to treat TB. Furthermore, there is a need to explore the activity of these tested plant against other pathogenic Mycobacterium species.

show abstract

Section: Methodsmentioning

confidence: 94%

Isolation of anti-mycobacterial compounds from Curtisia dentata (Burm.f.) C.A.Sm (Curtisiaceae)

Fadipe

Mongalo

Opoku

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…Phytochemically, the leaves of C. eludis revealed the presence of compounds such as β-amyrin, oleanolic acid, uvaol, monogalactosyldiacyl glycerol, catechin, epicatechin and procyanidin [59] . In the earlier reports, oleanolic acid isolated from other plant sources reveled an LC 50 of >300 μg/mL against both human embryonic kidney (HEK293) and hepatocellular carcinoma (HepG2) cell lines [60] . These findings may suggest that the cytotoxic effect of the plant species may not be due to the said phenolic acid alone but other compounds or in combination with those of the known compounds.…”

Section: Resultsmentioning

confidence: 97%

Antifungal, anti-oxidant activity and cytotoxicity of South African medicinal plants against mycotoxigenic fungi

Mongalo

Dikhoba

Soyingbe

et al. 2018

Heliyon

Self Cite

View full text Add to dashboard Cite

Fungal strains belonging to genus Fusarium and Aspergillus are known to infect crops, resulting in threatened food security and less agricultural crop yields. The aim of the current work was to investigate the anti-mycotoxigenic activity, cytotoxic effect and antioxidant potential of selected South African medicinal plants extracts. The aqueous and organic extracts of the leaves from selected medicinal plants were investigated for their antifungal activity against various fungal strains that are known to infect crops and produce mycotoxins. Antioxidant activity, total phenolic and total flavonoids contents were also evaluated. Organic extract of Milletia grandis (E. Mey) Skeels revealed the lowest minimum inhibitory concentration (MIC) value of 0.01 mg/mL against Aspergillus ochraceous, Fusarium graminearum and Furasium oxysporum. Generally, organic extracts revealed significant antifungal activity compared to aqueous extracts. Carpobrutus eludis L. and Warburgia salutaris (G. Bertol) Chiov. revealed a potent cytotoxic effect yielding 50 % lethal concentration (LC50) value of 0.01 mg/mL against Bovine dermis and Vero cells respectively. Ricinus communis L. revealed a 50 % inhibitory concentration (IC50) value of 945 μg/mL against 2, 2 diphenyl-picryl-hydrazyl (DPPH). Generally, the plant species revealed lower flavonoids compared to phenolic contents. The biological activity of the selected plant extracts may be attributed to the high phenolic contents.

show abstract

Health-promoting activities of clove (Syzygium aromaticum) extracts

Tomas¹,

Maroyi²,

Cheikhyoussef³

et al. 2022

Clove ( Syzygium Aromaticum)

View full text Add to dashboard Cite

Antimycobacterial, antiplasmodial and toxicological effect of oleanolic acid and its derivative from Syzygium aromaticum Linn (Myrtaceae)

Cited by 4 publications

References 0 publications

Isolation of anti-mycobacterial compounds from Curtisia dentata (Burm.f.) C.A.Sm (Curtisiaceae)

Isolation of anti-mycobacterial compounds from Curtisia dentata (Burm.f.) C.A.Sm (Curtisiaceae)

Antifungal, anti-oxidant activity and cytotoxicity of South African medicinal plants against mycotoxigenic fungi

Health-promoting activities of clove (Syzygium aromaticum) extracts

Contact Info

Product

Resources

About