Antifungal, anti-oxidant activity and cytotoxicity of South African medicinal plants against mycotoxigenic fungi

Mongalo, N.I.; Dikhoba, P.M.; Soyingbe, S.O.; Makhafola, Tshepiso Jan

doi:10.1016/j.heliyon.2018.e00973

Cited by 30 publications

(10 citation statements)

References 59 publications

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“…In the present study, Ziziphus mucronata did not show any cytotoxicity effects with LC 50 values of 2582.656 µg/ml against RAW 264.7 cells, however, it was toxic against C2C12 cells with LC 50 value of 150.210 µg/ml. Previous studies have reported cytotoxicity of Ziziphus mucronata with LC 50 value ranged from 0.10 µg/ml to 0.22 µg/ml against Bovine dermis and Vero cells (Mongalo et al, 2018). In other studies, no cytotoxicity was reported for Z. mucronata in RAW 264.7 cells with LC 50 value as low as >50 µg/ml.…”

Section: Discussionmentioning

confidence: 90%

The Evaluation Of Cytotoxicity And Anti-Inflammatory Effects Of Selected South African Medicinal Plants Against C2c12 Cells And Raw 264.7 Cells

B.A¹,

H.P²,

Qwebani‐Ogunleye³

2020

IJSRP

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Medicinal plants are used in traditional medicine throughout the world. In addition to this, certain communities consider medicinal plants to be safer than drugs and able to treat more than one ailment. This study aimed to evaluate the cytotoxicity and anti-inflammatory effects of Euclea crispa (leaf), Eulea natalensis (leaf), Schkuhria pinnata (leaf), Ziziphus mucronata (leaf), Ziziphus mucronata (fruits), Lippia javanica (leaf), Vernonia oligocephala (leaf), Clerodendrum myricoides (leaf), and Erythrina lysistemon (leaf) in C2C12, and RAW 264.7 cells. Plants were extracted with 90% methanol (1 g/10 ml) and diluted in distilled water to give a final concentration of 10 mg/ml. C2C12, and RAW 264.7 cells were treated for 24 h with various concentrations of plant extracts (10 -1000 µg/ml). Cytotoxicity was evaluated with Alamar Blue and crystal violet cell viability assays. RAW 264.7 cells were stimulated with lipopolysaccharide (LPS) to produce nitric oxide (NO). Thereafter, the antiinflammatory effect of the plant extracts was assessed by their ability to inhibit NO production, using the Griess reagent assay. None of the plants extracts demonstrated cytotoxic effects at the concentrations used against RAW 264.7 cells with LC 50 value >1000 µg/ml. However, a degree of cytotoxicity in all plant extracts against C2C12 cells in higher concentrations was observed with LC 50 <1000 µg/ml. All plant extracts demonstrated some degree of anti-inflammatory effect. However, plant extracts exhibited marked anti-inflammatory activities. These were Clerondendrum myricoides (35% -89%), Lippia javanica (26% -77%), Erythrina lysistemon (23% -76%), Schkuhria pinnata (27% -65%), and Vernonia oligocephala (16% -58%) with IC50 value >1000 µg/ml. The present findings suggest that these plants' extracts may serve as a promising therapeutic agent for inflammatory diseases and authenticates their use in traditional medicine.

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Section: Discussionmentioning

confidence: 90%

The Evaluation Of Cytotoxicity And Anti-Inflammatory Effects Of Selected South African Medicinal Plants Against C2c12 Cells And Raw 264.7 Cells

B.A¹,

H.P²,

Qwebani‐Ogunleye³

2020

IJSRP

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“…, Mahonia aquifolium Nutt. , Glycyrrhiza glabra L. and so on, were reported to exert antifungal activity [8–13]. In our screening study, we found that Maianthemum japonicum ( A.…”

Section: Introductionmentioning

confidence: 85%

Antifungal Activity of Crude Extract from the Rhizome and Root ofSmilacina japonicaA. Gray

Liu

Sun

Yang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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This study aimed to investigate the antifungal activity of hydroalcoholic extract from Smilacina japonica A. Gray (SJA) against different fungi. The minimum inhibitory concentration (MIC) for SJA was determined by the broth microdilution method. The antifungal effects of SJA against Candida albicans were further confirmed by cell growth test and time-kill curve test. The effects of SJA on the fungal morphology and ultrastructure were also evaluated. SJA has a broad-spectrum antifungal activity. The MICs of SJA against different fungi, including fluconazole-sensitive and -resistant Candida albicans, other Candida species, and Cryptococcus neoformans, ranged from 208 μg/ml to 1665 μg/ml. Furthermore, SJA displayed fungicidal activity against varied fungi and obviously inhibited the hyphal growth of fungi. The mechanism study revealed that the antifungal activity of SJA might be associated with its effect on the cell morphology and ultrastructure.

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“…The phytochemicals that produce medicinal plants derived from their secondary metabolism represent a safe and effective alternative to control various phytopathogens that affect various crops of agricultural products of economic and nutritional Table 4. Secondary metabolites of medicinal plants with biological activity against phytopathogens [86][87][88][89][90][91][92][93][94].…”

Section: Discussionmentioning

confidence: 99%

Plant Metabolites in Plant Defense Against Pathogens

Ramírez-Gómez¹,

Jiménez-García²,

Campos³

et al. 2020

Plant Diseases - Current Threats and Management Trends

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Medicinal plants are widely used worldwide to treat various diseases. Its widespread use is due in part to the cultural acceptance of traditional medicine in different regions of the world, as well as its effectiveness in treating various diseases. Many of its active substances or secondary metabolites are formed to a response of various situations that generate stress in their habitat, such as sudden changes in environmental temperature, humidity, rain, drought, and infections by phytopathogens (fungi, bacteria, viruses, nematodes, protozoa). The production of these secondary metabolites is a mechanism of defense of plants. In this context, the objective of this chapter is to study the secondary metabolites of medicinal plants that could have a promising application in the control of different phytopathogens in crops of agricultural and economic interest.

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Antifungal, anti-oxidant activity and cytotoxicity of South African medicinal plants against mycotoxigenic fungi

Cited by 30 publications

References 59 publications

The Evaluation Of Cytotoxicity And Anti-Inflammatory Effects Of Selected South African Medicinal Plants Against C2c12 Cells And Raw 264.7 Cells

The Evaluation Of Cytotoxicity And Anti-Inflammatory Effects Of Selected South African Medicinal Plants Against C2c12 Cells And Raw 264.7 Cells

Antifungal Activity of Crude Extract from the Rhizome and Root ofSmilacina japonicaA. Gray

Plant Metabolites in Plant Defense Against Pathogens

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