Antimutagenic Effect ofHibiscus sabdariffaL. Aqueous Extract on Rats Treated with Monosodium Glutamate

Abstract

Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25 mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract of H. sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested, H. sabdariffa L. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis.

Phyto-chemistry and Pharmacological Activities of Hibiscus sabdariffa Linn.-A Review

“…In another study the effects of Hibiscus sabdariffa aqueous extract had been evaluated against cyclophosmsmide (CPA) on male Wistar rats treated with monosodium glutamate. The group treated with the aqueous extract of Hibiscus sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group and it is a potential candidate as a chemopreventive agent against carcinogenesis [72].…”

“…The investigation for the anticancer potential of the Hibiscus sabdariffa plant began roughly in the last decade that emerged with encouraging results [63][64][65][66][67][68][69]. Both crude extracts and pure compounds of the plant were reported to induce chemoprevention, selective cytotoxicity, cell cycle arrest, apoptosis, autophagy and antimetastatis effects in various types of human cancer cells.…”

“…Several researchers reported the cytotoxic, antimutagenic, antimetastatic, antiproliferative, apoptotic antiangiogenic activity of Hibiscus sabdariffa both in human and animal cancer cells. This activity of Hibiscus sabdariffa is due to the presence of polyphenols in it, which attributes towards its antioxidant and anticarcinogenic activities [64][65][66][67][68][69]. The anticancer potential of Hibiscus sabdariffa leaves extracts (methanol, ethanol, ethyl acetate and chloroform) on hepatocellular carcinoma cell line Hep 3B was tested and methanolic extract showed a greater cytotoxic effect when compared with others [70].…”

Organic Acids from Roselle (Hibiscus sabdariffa L.)—A Brief Review of Its Pharmacological Effects

“…(e) The aqueous extracts have been the most studied so far, and this is where the genoprotective effect of some phenolic and flavonoid acids of jamaica has been achieved. With the purpose to determine the antimutagenic potential of an aqueous extract of Hibiscus sabdariffa L., Brazilian researchers (2016) [8] conducted an in vivo study. They used Wistar rats that were treated with the extract (400 mg/kg) for 15 days and subsequently a single dose of cyclophosphamide (25 mg/kg).…”

Ethnomedicinal, Phytochemical and Ethnopharmacological Aspects of Four Medicinal Plants of Malvaceae Used in Indian Traditional Medicines: A Review

“…Aqueous extract of calyx of H. sabdariffa showed chemoprotective effects in cyclophosphamide- induced DNA damage in male Wistar rats, thus indicating anti-mutagenic activity against chemical (cyclophosphamide)- induced carcinogenesis [ 169 ].…”