Antimutagenic, Antiproliferative and Antioxidant Properties of Sea Grape Leaf Extract Fractions (Coccoloba uvifera L.)

Abstract

Background: Cancer is a disease characterized by the invasion and uncontrolled growth of cells. One of the best ways to minimize the harmful effects of mutagens is through the use of natural antimutagens. In this regard, the search for new antimutagens that act in the chemoprevention could represent a promising field in this area. Objective: In this study biological potential of 11 fractions from Coccoloba uvifera L. leaf hexane extract was evaluated by several in vitro tests. Methods: Leaves were lyophilized and hexane extraction was performed. The extract was fractionated by column chromatography with hexane, ethyl acetate, and methanol. The antimutagenic (Ames test), antiproliferative (MTT test), and antioxidant capacity (DPPH, ABTS, and ferrous ion chelation) of the fractions were evaluated. Results: Fractions 4, 6, 8, and 9 have antimutagenic activity (against sodium azide in strain TA100), fraction 11 showed antiproliferative capacity (IC50 of 24 ± 9 μg/mL in cells of HCT 116). The fractions with the highest activity were analyzed by HPLC-MS and lupeol, acacetin, and β-sitosterol were identified. Conclusion: This study demonstrates, for the first time, the bioactivity of C. uvifera leaf as a new source of high biological value compounds (HBVC), which can be of interest to the food and pharmaceutical industries.

Microencapsulation of Phenolic Extract from Sea Grape (Coccoloba uvifera L.) with Antimutagenic Activity

“…Antimutagenic compounds contribute to cancer prevention, because they decrease the concentration of mutagens, blocking mutagens before they damage DNA. [47] The inhibition of spontaneous revertants obtained in this research is higher than the previously reported fractions of jackfruit methanol-hexane extract at 50 mg/mL [48] and similar to fresh [47] and microencapsulated sea grape leaves extract in nanofibers of gelatin/agave fructans, [49] both at 20 μg/plate. The sea grape leaves extract is rich in triterpenes such as lupeol and eucomic, piscidic, caffeic, ferulic, and quinic organic acids.…”

“…The inhibition of spontaneous revertants obtained in this research is higher than the previously reported fractions of jackfruit methanol‐hexane extract at 50 mg/mL [48] and similar to fresh [47] and microencapsulated sea grape leaves extract in nanofibers of gelatin/agave fructans, [49] both at 20 μg/plate. The sea grape leaves extract is rich in triterpenes such as lupeol and eucomic, piscidic, caffeic, ferulic, and quinic organic acids [47] . The differences in the antimutagenic activity between this and other studies are associated with the source and type of compounds present in the extracts, purity degree, extraction method, and so on.…”

“…These results provide the first approach for future application of the microencapsulated sea grape extract as a chemopreventive in agent food systems or in biomedicine. Antimutagenic compounds contribute to cancer prevention, because they decrease the concentration of mutagens, blocking mutagens before they damage the DNA [47] . The inhibition of spontaneous revertants obtained in this research is higher than the previously reported fractions of jackfruit methanol‐hexane extract at 50 mg/mL [48] and similar to fresh [47] and microencapsulated sea grape leaves extract in nanofibers of gelatin/agave fructans, [49] both at 20 μg/plate.…”

“…The sea grape leaves extract is rich in triterpenes such as lupeol and eucomic, piscidic, caffeic, ferulic, and quinic organic acids. [47] The differences in the antimutagenic activity between this and other studies are associated with the source and type of compounds present in the extracts, purity degree, extraction method, and so on.…”

Encapsulation with HDPAF-WP of the hexane fraction of sea grape (Coccoloba uvifera L.) leaf extract by electrospraying

“…These compounds are of great biological interest since they offer great benefits to the consumers, such as, anti-inflammatory, anti-cancer, and gastroprotective. Ramos-Hernández et al [10], identified fractions of lupeol, acecetin, and βsitosterol of sea grape extract by column chromatography purification. These compounds possess antimutagenic, antiproliferative, and antioxidant properties.…”