Antimutagenic, antiproliferative, and antioxidant effect of extracts obtained from octopus (Paraoctopus limaculatus)

Abstract

The search for chemopreventive/chemoprotective compounds in marine organism has been extensively reported; however, the presence of these compounds in octopus has been incipiently explored. In this research, the antimutagenic, antiproliferative, and antioxidant potential of three crude extracts (methanolic, acetonic, and hexanic) from Paroctopus limaculatus was investigated. Antimutagenic activity against aflatoxin B 1 (AFB 1 ) was evaluated through the Ames test using Salmonella typhimurium tester strains TA98 and 100. Antiproliferative activity was assessed using the standard MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay on M12.C3.F6 murine cell line. Antioxidant activity was assessed using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) methods. Hexanic extract showed the highest antimutagenic and antiproliverative activities inhibiting 80 and 43% of mutagenicity induced by AFB 1 for TA98 and TA100, respectively, and showing a high antiproliferative activity at 200 and 100 µg/mL. However, when the antioxidant activity was evaluated at a concentration of 50 mg/mL, the methanolic fraction exerted inhibition of 98 and 96 % ABTS and DPPH radicals, respectively. RP-HPLC and 1 H-RMN analyses suggested the presence of double bonds with extended conjugation and oxygenated compounds such as alcohols, esters, ethers or ketones. These results suggested that hexanic and methanolic extract form octopus contained compounds with chemoprotective and antioxidant properties.Keywords: octopus; biological activity; chemopreventive.Practical Application: Octopus fractions can be used as potential chemopreventive and antioxidant compounds.

Chemical structure and antioxidant activity of cephalopod skin ommochrome pigment extracts

“…Therefore, the antioxidant activity of the pigmented extracts was considered adequate, particularly that of methanolic extracts. These results are supported by the work of Cruz-Ramírez et al (2015), who reported that methanol is one of the most effective organic solvents for extracting bioactive compounds from octopus (Paraoctopus limaculatus). Although more studies are necessary, the high antioxidant potential of extracts obtained using methanol-HCl could be explained by the high presence of aromatic protons and amino group protons in these extracts.…”

“…The order of the yield after drying was OVS-T1 > DGS-T1 > OVS-T2 > DGS-T2. Similarly, Cruz-Ramírez et al (2015) reported that methanol was one of the most effective organic solvents for the extraction of compounds from an octopus.…”

“…Most solvents used to extract bioactive compounds from cephalopods are aqueous solutions of ethanol, methanol, acetone, and hexane; for example, ethanolic extracts of squid (Loligo duvauceli) ink exhibited higher antioxidant activity than hexane extracts (Fatimah & Rabeta, 2017). In contrast, antimutagenic activity assessed in octopus (Paraoctopus limaculatus) tentacle extracts was higher in hexane than in methanol and acetone extracts (Cruz-Ramírez et al, 2015). Until now, pigmented extracts…”

Octopus vulgaris ink extracts exhibit antioxidant, antimutagenic, cytoprotective, antiproliferative, and proapoptotic effects in selected human cancer cell lines

“…A similar trypsin fractioning was also used to hydrolyze squid ( Ommatresphes bartrami ) oligopeptides for producing a proapoptotic tripeptide (Huang et al., 2012), suggesting that the ink hydrolysis by similar enzymes as those found in the gastrointestinal tract might potentiate the bioactivity of its components. Other parts from octopus have also shown antimutagenic activity, such as freeze‐dried tentacles, attributed to the content of 1‐butyl‐2‐isobutyl phthalate isolated from the lipid fraction of the muscle (Cruz‐Ramírez et al., 2015).…”

Antimutagenic bis (2-ethylhexyl) phthalate isolated from octopus (Paraoctopus vulgaris)

“…Marine invertebrates, especially cephalopods, constitute an important source of many biological substances with antioxidant, antitumor, antileukemic, antibacterial, and antiviral activities (Sudhakar & Nazeer, 2015;Jesy et al, 2014;Jayaraj et al, 2008;Rajaganapathi et al, 2000). In particular, Paraoctopus limaculatus has been reported as a source of bioactive agents since the isolation of extracts with chemopreventive activity has previously been carried out (Cruz-Ramírez et al, 2015), suggesting the presence of antimutagenic and antiproliferative hexane-soluble compounds. Therefore, the aims of this study were to chemical and structurally characterize the antimutagenic compounds present in the hexane-soluble fraction of octopus tentacles.…”