Antimicrobials from Microbes

Francis, Dileep

doi:10.1007/978-981-10-4284-3_12

Cited by 6 publications

(2 citation statements)

References 32 publications

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“…including bacitracin (Bacillus megaterium, Bacillus subtilis, and Bacillus licheniformis), lichenin (Bacillus licheniformis), megacin (Bacillus megaterium), coagulin (Bacillus coagulans), polyfermenticin (Bacillus polyfermenticus), cerein (Bacillus cereus), thuricins, tochicin, kurstakin, entomocin and bacthuricin (Bacillus thuringiensis). [39][40][41] The chemical signal molecules (autoinducers) were produced and released from quorum sensing bacteria in response to change the cell populatin density. 42 While there are many types of signaling molecules produced by bacteria, Autoinducer-1 (AHLs -N-acyl homoserine lactones) derived from gram-negative bacteria is perhaps the most interesting.…”

Section: Effect Of Cyb On Total Vibrio Count In Culture Watermentioning

confidence: 99%

Effect from Combinations of Yucca schidigera extract with Bacillus Strains on the Growth of White Leg Shrimp (Penaeus vannamei), Density of Vibrio sp., and on the Ammonia and Nitrite Content of Culture Water

Phuc,

Huong,

Dan

et al. 2023

J. Pure Appl. Microbiol.

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The water quality including toxic gas parameters and Vibrio density is a serious problem in intensive shrimp culture. Yucca schidigera extract and Bacillus sp. are considered as a practical solution to improve the water quality. The aim of our study was to determine the effect of combination of Yucca schidigera extract and Bacillus spp. on total ammonia nitrogen (TAN) and nitrite contents, Vibrio count, and the growth performance of white leg shrimp (Penaeus vannamei). Shrimp (PL12) were assigned to seven treatments (triplicates) including treatments with combination of yucca extract and single strain of Bacillus (AY, B2Y, B3Y), combination of yucca extract and Bacillus consortium (ABBY), yucca extract only (Y), Bacillus consortium (ABB), and control treatment (C). Vibrio count was carried out by the spread plate technique every 7 days. TAN and nitrite contents were determined by using sodium nitroprusside, and sulphanilic acid with N-(1-naphthyl) ethylenediamine, respectively, at 24 hours and 168 hours after adding Bacillus strains and yucca extract. The shrimp growth parameters include the final biomass, final body weight (FBW, g), weight gain rate (WGR, %), survival rate (SR, %), and feed conversion ratio (FCR) were recorded after 5 weeks. The results showed that the water quality (0.073 – 0.179 ppm of TAN, 0.187 – 0.934 ppm of nitrite, and Vibrio count of 2.552 – 3.038 log CFU/ml) of tanks treated with combination of yucca extract and single strain of Bacillus (AY, B2Y, B3Y) or Bacillus consortium (ABBY), and Bacillus consortium (ABB) was significantly controlled compared to the control and yucca extract only treatments. The ABBY treatment most significantly improved the shrimp growth parameters (FBW, biomass, WGR, FCR, and SR – 0.424 ± 0.010 g, 40.202 ± 1.510 g, 14048.902 ± 328.756 %, 1.100 ± 0.040, and 94.667 ± 1.856 %, respectively) compared to the other treatments. Overall, our study concluded that the combination of yucca extract and Bacillus consortium could control water quality (0.036 – 0.105 ppm of TAN, 0.187 – 0.604 ppm of nitrite, and Vibrio count of 2.553 – 2.624 log CFU/ml), reduce 46.49% of FCR and significantly increase the growth performance (approximately 92.73% of FWB, 163.19% of biomass, 93.93% of WGR, 46.49% of FCR) of P. vannamei (PL12) with density of 100 individuals/100L.

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Section: Effect Of Cyb On Total Vibrio Count In Culture Watermentioning

confidence: 99%

Effect from Combinations of Yucca schidigera extract with Bacillus Strains on the Growth of White Leg Shrimp (Penaeus vannamei), Density of Vibrio sp., and on the Ammonia and Nitrite Content of Culture Water

Phuc,

Huong,

Dan

et al. 2023

J. Pure Appl. Microbiol.

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“…Microorganisms are a rich source of antimicrobial compounds [ 9 – 11 ] that are produced as an outcome of various competitive mechanisms including microbial antagonism [ 12 , 13 ]. Microorganisms may cause inhibition by changing pH, osmotic pressure and surface tension or by producing toxic components, antibiotics, bacteriocins etc.…”

Section: Introductionmentioning

confidence: 99%

Anti-mycobacterial activity of heat and pH stable high molecular weight protein(s) secreted by a bacterial laboratory contaminant

et al. 2022

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Background Tuberculosis currently stands as the second leading cause of deaths worldwide due to single infectious agent after Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The current challenges of drug resistance in tuberculosis highlight an urgent need to develop newer anti-mycobacterial compounds. In the present study, we report the serendipitous discovery of a bacterial laboratory contaminant (LC-1) exhibiting a zone of growth inhibition on an agar plate seeded with Mycobacterium tuberculosis. Results We utilized microbiological, biochemical and biophysical approaches to characterize LC-1 and anti-mycobacterial compound(s) in its secretome. Based on 16S rRNA sequencing and BIOLOG analysis, LC-1 was identified as Staphylococcus hominis, a human bacterial commensal. Anti-mycobacterial activity was initially found in 30 kDa retentate that was obtained by ultrafiltration of culture filtrate (CF). SDS-PAGE analysis of peak fractions obtained by size exclusion chromatography of 30 kDa retentate confirmed the presence of high molecular weight (≥ 30 kDa) proteins. Peak fraction-1 (F-1) exhibited inhibitory activity against M. bovis BCG, but not against M. smegmatis, E. coli and S. aureus. The active fraction F-1 was inactivated by treatment with Proteinase K and α-chymotrypsin. However, it retained its anti-mycobacterial activity over a wide range of heat and pH treatment. The anti-mycobacterial activity of F-1 was found to be maintained even after a long storage (~12 months) at − 20 °C. Mass spectrometry analysis revealed that the identified peptide masses do not match with any previously known bacteriocins. Conclusions The present study highlights the anti-mycobacterial activity of high molecular weight protein(s) present in culture filtrate of LC-1, which may be tested further to target M. tuberculosis. The heat and pH stability of these proteins add to their characteristics as therapeutic proteins and may contribute to their long shelf life. LC-1 being a human commensal can be tested in future for its potential as a probiotic to treat tuberculosis.

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Microbial Technology in Bioplastic Production and Engineering

Francis¹,

Parayil²

2022

Handbook of Bioplastics and Biocomposites Engineering Applications

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Antimicrobials from Microbes

Cited by 6 publications

References 32 publications

Effect from Combinations of Yucca schidigera extract with Bacillus Strains on the Growth of White Leg Shrimp (Penaeus vannamei), Density of Vibrio sp., and on the Ammonia and Nitrite Content of Culture Water

Effect from Combinations of Yucca schidigera extract with Bacillus Strains on the Growth of White Leg Shrimp (Penaeus vannamei), Density of Vibrio sp., and on the Ammonia and Nitrite Content of Culture Water

Anti-mycobacterial activity of heat and pH stable high molecular weight protein(s) secreted by a bacterial laboratory contaminant

Microbial Technology in Bioplastic Production and Engineering

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