Microbial Technology in Bioplastic Production and Engineering

Francis, Dileep; Parayil, Deepu Joy

doi:10.1002/9781119160182.ch6

Cited by 1 publication

(2 citation statements)

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“…Conducted in bioreactors under controlled conditions, these fed‐batch bioprocesses are typically divided into a growth phase, where the bacterial culture is allowed to reach high cell densities before nutrient stress is imposed to initiate a production phase, which results in a switching of metabolic pathways in favor of PHA production to achieve the highest volumetric productivities possible. For certain microorganisms like Alcaligenes latus and recombinant Escherichia coli , the growth and PHA production phases occur simultaneously 36–38 . Techniques for PHA recovery include cell separation from the culture (centrifugation, cross‐flow filtration, flotation, etc .)…”

Section: Pha Production In Brazil: Challenges and Directionsmentioning

confidence: 99%

“…For certain microorganisms like Alcaligenes latus and recombinant Escherichia coli, the growth and PHA production phases occur simultaneously. [36][37][38] Techniques for PHA recovery include cell separation from the culture (centrifugation, cross-flow filtration, flotation, etc.) followed by solvent-based extraction and precipitation or non-solvent-based methods involving mechanical disruption of cells or their digestion with enzymes or hypochlorite followed by additional steps for polymer purification.…”

Section: Introduction -Bioplastics Worldwidementioning

confidence: 99%

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Cross‐sectoral citrus sugarcane biorefinery: polyhydroxyalkanoates production in Brazil

Kanbe,

da Silva,

Gomez

2023

Biofuels Bioprod Bioref

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Polyhydroxyalkanoates (PHA) are naturally occurring polyesters accumulated by bacteria under conditions of inorganic nutrient limitations and excess carbon. They offer renewable and biodegradable alternatives to petroleum‐based polymers, especially those used in transient single‐use applications. However, PHA still faces high production costs, primarily linked to the carbon source, lower productivity in processes and an early stage of technical development in comparison with well‐established fossil‐based polymers, which challenge its transition to a true commodity market. Recent research has highlighted the potential of renewable, inexpensive, abundant and non‐edible carbon‐rich residues from agriculture, municipal solid waste and industrial byproducts as alternative carbon sources in developing the production process. Recognizing Brazil's position as the leading producer of both citrus and sugarcane, this review introduces a novel citrus–sugarcane biorefinery model for PHA production, capitalizing on the geographical and operational synergies of both sectors. The proposed model integrates wet citrus pulp as a second‐generation carbon source, sugarcane industry fermenters, waste management, solvents and renewable energy from both sectors. Primary challenges involve the need for efficient, low‐cost biomass pre‐treatment to remove limonene and partially extract citric pectin, coupled with the partial hydrolysis of pectin to enhance solubility and reduce viscosity. Additional considerations include determining citrus biomass composition and sugar consumption, screening bacterial diversity for optimal PHA production, and deepening our understanding of the metabolic pathways underpinning the catabolism of citrus residues. Future research should focus on enhancing PHA concentrations and diversity and finally conducting life cycle assessment studies to evaluate the sustainability and feasibility of this proposed model.

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Section: Pha Production In Brazil: Challenges and Directionsmentioning

confidence: 99%

Section: Introduction -Bioplastics Worldwidementioning

confidence: 99%