Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils

Nikolić, Miloš; Jovanović, Katarina; Marković, Tatjana; Marković, Dejan; Gligorijević, Nevenka; Radulović, Siniša; Kostić, Milena; Glamočlija, Jasmina; Sokóvić, Marina

doi:10.1111/jphp.12792

Cited by 43 publications

(31 citation statements)

References 38 publications

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“…On the other hand, fungi strains of A. niger and S. cerevaceae were found to display moderate activity with the zone of 9 mm. The present results are higher than reported data (Bag & Chattopadhyay, ; Nikolic et al, ) whereas, some oils were reported to have higher activity (Morsy & El‐Salam, ; Singh et al, ). This variation is due to the major component present in the oils.…”

Section: Resultscontrasting

confidence: 91%

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Chemical, pharmacological and nutritional quality assessment of black pepper (Piper nigrumL.) seed cultivars

et al. 2018

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The present research has been performed on black pepper of two cultivars to evaluate their nutritional quality and safety issue on the basis of proximate, chemical and pharmacological properties. The proximate results were compiled with the standard USDA limits. K, Ca, Mg, Na, Fe, Al and Se were detected as major elements, whereas the toxic elements were found within the recommended limit. The GC‐MS analysis of the essential oils showed the presence of δ‐3‐carene (32.61%) as the major component in Kerala cultivar whereas β‐caryophyllene (18.39%) was the major components in Indigenous cultivar. The IC50 values of DPPH antioxidant activity of the essential oils were found to be 44.16 and 22.88 mg/mL in Indigenous and Kerala cultivars, respectively. The antimicrobial activity of the essential oils showed good activity in both cultivars. The LC50 values of the brine shrimp cytotoxic activity were 1.03 and 1.21 μg/mL in Indigenous and Kerala cultivars, respectively. Practical applications Spice quality is an important issue in the food industries as well as export markets. Due to the toxicological effect of imported spices, much attention has been paid to the safety issue especially on cytotoxicity and toxic metal contaminations. The present study reveals that both cultivars possess rich amount of nutritional components, essential minerals, phyto and flavouring components in their essential oils and recommended level of toxic elements as well as decent antioxidant and antimicrobial properties. This study promises to scaffold a new window for exportation of quality spice and confer potential for use in food, nutraceutical and pharmaceutical industries.

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Section: Resultscontrasting

confidence: 91%

“…Moreover, Krishnaraju et al () reported the LC 50 30 μg/mL at the dose of 1–5000 μg/mL. In another report, the LC 50 values were found to have 25.57–56.74 μg/mL on the cell line assay (Nikolic et al, ). The observed difference may be due to the chemical composition of the oils as well as synergistic action.…”

Section: Resultsmentioning

confidence: 97%

Chemical, pharmacological and nutritional quality assessment of black pepper (Piper nigrumL.) seed cultivars

et al. 2018

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“…The product should not cause death of the cells, nor affect their cellular functions, as was observed in this work. Nicolic et al studied the cytotoxic activity of lemon essential oil ( Citrus limon L.) using the human fetal lung fibroblast cell line derived from the test of 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium (MTT) and confirmed the absence of cytotoxic activity, which agrees with the results obtained in the present work.…”

Section: Resultssupporting

confidence: 90%

Biological activities of the essential oil from the Moro orange peel (Citrus sinensis (L.) Osbeck)

Magalhães

Ionta

Ferreira

et al. 2019

Flavour & Fragrance J

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Citrus essential oils have become the focus of several researches, because they have broad biological activity, due to their chemical composition. However, there are few studies covering Moro orange essential oil. Thus, the aim of this study was to evaluate the effects of fresh Moro orange peel essential oil on antifungal, antioxidant, cytotoxic, and genotoxic activities in normal and tumor cells. The essential oil was extracted by hydrodistillation and characterized by GC/MS and GC/FID. Cytotoxicity was evaluated by MTS assay in normal cells (CCD‐1059Sk) and tumor cells (CCD‐1059Sk), and the genotoxicity was determined in normal cells by the comet assay. Antifungal activity was evaluated by the disk diffusion test against Aspergillus carbonarius and Aspergillus flavus filamentous fungi. The antioxidant activity was determined in DPPH, ABTS, and β‐carotene assay. The essential oil was mainly composed of monoterpenes such as limonene (95.12%), α‐pinene (0.35%), sabinene (0.54%), and myrcene (1.07%). The essential oil did not cause cell death after 48 hours of treatment in normal cells, but the cell viability of tumor cells decreased by 50% in the presence of the essential oil at a concentration of 272.6 μg/mL. Although no antioxidant effect was observed, the growth of all evaluated fungi was inhibited with a minimum inhibitory concentration (MIC) of 125 μL/mL. The essential oil from the Moro orange is promising for cancer treatment, and it can be used as a natural preservative in food systems.

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“…Among the analysed metabolites, sabinene, β-myrcene, δ-3-carene, γ-terpinene, limonene, α-capaene, octanoic acid, and isocaryophillene were reported to have antimicrobial properties. [14][15][16][17][18][19] Identified metabolites reported to have antimicrobial and antioxidant properties are also tabulated in Table 5. Figure 4A).…”

Section: Gc-ms Analysis Of Eomentioning

confidence: 99%

TLC‐bioautography identification and GC‐MS analysis of antimicrobial and antioxidant active compounds in Musa × paradisiaca L. fruit pulp essential oil

Fahim

Ibrahim

Zahiruddin

et al. 2019

Phytochemical Analysis

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Introduction The absence of microbial growth and resistance to oxidative deterioration in fruits of Musa × paradisiaca L. (bananas) is an indication of the presence of antimicrobial and antioxidant metabolites. Objective In order to investigate the secondary metabolomic spectrum as well as the active antimicrobial and antioxidants present in essential oils (EOs) from fruits of different geographical areas of M. × paradisiaca, gas chromatography‐mass spectroscopy (GC‐MS) principal component data correlation analysis is complemented with antimicrobial assays and phytochemical and bioautographic antioxidant fingerprints with thin layer chromatography (TLC). Methodology An EO was obtained by steam distillation and subjected to GC‐MS and TLC for metabolomic profiling from fruit pulp. The antimicrobial potential was tested in both Escherichia coli as a gram negative and Bacillus subtilis as a gram positive microbe. Potential antioxidant metabolites were identified through TLC‐bioautography and GC‐MS analysis of active zones. Results A maximum of 0.56% v/w EO was isolated from fruit pulps of M. × paradisiaca. Minimum inhibitory concentrations (MICs) against B. subtillis and E. coli were 0.25 and 0.35 μg/mL, respectively. Thus, 56 metabolites were identified through GC‐MS. The major abundant antimicrobial metabolites found in EOs are α‐thujene, γ‐terpinene, α‐ and β‐pinene, sabinene, β‐myrcene, limonene, α‐capaene, caryophyllene and (Z,E)‐α farnesene. Aceteugenol, palmitic acid, stearic acid, palmitin, and stearin were identified as antioxidant metabolites. Principal component analysis of metabolite data reveals correlations and a clear separation based on metabolites obtained from various areas. Conclusion The data generated using metabolic profiling and cluster analysis helped to identify antimicrobial and antioxidant compounds in M. × paradisiaca.

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Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils

Abstract: The results of this study encourage use of tested EOs in development of a novel agent intended for prevention or therapy of corresponding oral disorders.

Cited by 43 publications

References 38 publications

Chemical, pharmacological and nutritional quality assessment of black pepper (Piper nigrumL.) seed cultivars

Chemical, pharmacological and nutritional quality assessment of black pepper (Piper nigrumL.) seed cultivars

Biological activities of the essential oil from the Moro orange peel (Citrus sinensis (L.) Osbeck)

TLC‐bioautography identification and GC‐MS analysis of antimicrobial and antioxidant active compounds in Musa × paradisiaca L. fruit pulp essential oil

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