Antimicrobial Susceptibility Pattern of Helicobacter heilmannii and Helicobacter ailurogastricus Isolates

Matos, Rita; Witte, Chloë De; Smet, Annemieke; Berlamont, Helena; Bruyckere, Sofie De; Amorim, Irina; Gärtner, Fátima; Haesebrouck, Freddy

doi:10.3390/microorganisms8060957

Cited by 15 publications

(20 citation statements)

References 49 publications

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“…[4][5][6] Gastric NHPH species are mainly found in the stomach of several animals, such as dogs, cats, and pigs, 15 but several of these bacteria can also colonize the human stomach. [10][11][12][13] Compared with H. pylori, NHPH infection rate is lower, being found in 0.2%-6.0% of gastric biopsies from human patients with gastric disease. 15 In our experiments, we were able to observe, for the first time, in vitro binding of several NHPH strains to the human gastric mucosa.…”

Section: Discussionmentioning

confidence: 99%

Helicobacterspecies binding to the human gastric mucosa

et al. 2021

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Helicobacter pylori infects half of the world population, being associated with several gastric disorders, such as chronic gastritis and gastric carcinoma. The Helicobacter genus also includes other gastric helicobacters, such as H. heilmannii¸ H. ailurogastricus, H. suis, H. felis, H. bizzozeronii, and H. salomonis. These gastric helicobacters colonize both the human and animal stomach. The prevalence of gastric non-Helicobacter pylori Helicobacter (NHPH) species in humans has been described as low, and the in vitro binding to the human gastric mucosa was never assessed. Herein, human gastric tissue sections were used for the evaluation of the tissue glycophenotype and for the binding of gastric NHPH strains belonging to different species. Histopathological evaluation showed that 37.5% of the patients enrolled in our cohort presented chronic gastritis, while the presence of neutrophil or eosinophilic activity (chronic active gastritis) was observed in 62.5% of the patients. The secretor phenotype was observed in 68.8% of the individuals, based on the expression of Lewis B antigen and binding of the UleX lectin. The in vitro binding assay showed that all the NHPH strains evaluated were able to bind, albeit in low frequency, to the human gastric mucosa. The H. heilmannii, H. bizzozeronii, and H. salomonis strains displayed the highest binding ability both to the gastric superficial epithelium and to the deep glands. Interestingly, we observed binding of NHPH to the gastric mucosa of individuals with severe chronic inflammation and intestinal metaplasia, suggesting that NHPH binding may not be restricted to the healthy gastric mucosa or slight chronic gastritis. Furthermore, the in vitro binding of NHPH strains was observed both in secretor and non-secretor individuals in a similar frequency. In conclusion, this study is the first report of the in vitro binding ability of gastric NHPH species to the human gastric mucosa. The results suggest that other glycans, besides the Lewis antigens, could be involved in the bacterial adhesion mechanism; however, the molecular intervenients remain unknown.

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Section: Discussionmentioning

confidence: 99%

Helicobacterspecies binding to the human gastric mucosa

et al. 2021

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“…Indeed, the majority of the pathogens are able to modulate the expression of their lectins, in order to adapt to alterations in the host glycosylation profile observed for several diseases (Ringot-Destrez et al, 2017). Glycans have been implicated in host-pathogen interactions of several microorganisms, including viruses such as Influenza virus (Bateman et al, 2010;Air, 2014;Cohen et al, 2016;Byrd-Leotis et al, 2017;Mayr et al, 2018), Calicivirus (Bhella et al, 2008), and recently SARS-Cov-2 (Casalino et al, 2020;Clausen et al, 2020;Watanabe et al, 2020), as well as bacteria, such as Mycobacterium tuberculosis (Barnes et al, 2017;Shukla et al, 2018;Fonseca et al, 2020;Matos et al, 2020), Pseudomonas eruginosa (Linden et al, 2008), Escherichia coli (Krishnan et al, 2014;Bottomley et al, 2020), Yersinia pestis, Yersinia pseudotuberculosis and Yersinia enterocolitica (Pakharukova et al, 2016) and H. pylori (Borén et al, 1994;Aspholm-Hurtig et al, 2004;Benktander et al, 2012).…”

Section: Role Of Glycans In Host-pathogen Interactionmentioning

confidence: 99%

“…The efficacy of the conventional H. pylori eradication therapy, usually a combination of 2-3 antibiotics with an acid-suppressive drug (Chey et al, 2017), has been declining in the last years, and the WHO considered priority the search for new drugs and alternative therapies (De et al, 2009;Blaecher et al, 2013). One of the major problems has been the acquisition of antimicrobial resistance, not only for H. pylori, but also for some NHPH such as H. heilmannii, H. ailurogastricus, H. felis and H. suis (Van den Bulck et al, 2005;Berlamont et al, 2019;Matos et al, 2020). The analysis of the antimicrobial susceptibility pattern is important to better understand the factors underneath the increasing resistance and to improve the available treatment.…”

Section: Helicobacter Pylori Adhesion To Human Gastric Mucosa: An Interaction Mediated By Glycansmentioning

confidence: 99%

“…H. pylori is the most prevalent Helicobacter species in the human stomach, however other gastric, spiral-shaped NHPH have also been associated with the development of gastric disorders in humans (Baele et al, 2008;Ali et al, 2018;Matos et al, 2020). NHPH have been detected in 0.2-6% of the human gastric biopsies, usually accompanied by gastritis, antral erosions, duodenal ulcers and gastric MALT lymphoma (Morgner et al, 2000;Haesebrouck et al, 2009;Joosten et al, 2016;Ali et al, 2018).…”

Section: Non-helicobacter Pylori Helicobacters (Nhph) Adhesion: What Is the Mechanism And Which Molecules Are Involved?mentioning

confidence: 99%

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Adhesion of Helicobacter Species to the Human Gastric Mucosa: A Deep Look Into Glycans Role

Matos

Amorim

Magalhães

et al. 2021

Front. Mol. Biosci.

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Helicobacter species infections may be associated with the development of gastric disorders, such as gastritis, peptic ulcers, intestinal metaplasia, dysplasia and gastric carcinoma. Binding of these bacteria to the gastric mucosa occurs through the recognition of specific glycan receptors expressed by the host epithelial cells. This review addresses the state of the art knowledge on these host glycan structures and the bacterial adhesins involved in Helicobacter spp. adhesion to gastric mucosa colonization. Glycans are expressed on every cell surface and they are crucial for several biological processes, including protein folding, cell signaling and recognition, and host-pathogen interactions. Helicobacter pylori is the most predominant gastric Helicobacter species in humans. The adhesion of this bacterium to glycan epitopes present on the gastric epithelial surface is a crucial step for a successful colonization. Major adhesins essential for colonization and infection are the blood-group antigen-binding adhesin (BabA) which mediates the interaction with fucosylated H-type 1 and Lewis B glycans, and the sialic acid-binding adhesin (SabA) which recognizes the sialyl-Lewis A and X glycan antigens. Since not every H. pylori strain expresses functional BabA or SabA adhesins, other bacterial proteins are most probably also involved in this adhesion process, including LabA (LacdiNAc-binding adhesin), which binds to the LacdiNAc motif on MUC5AC mucin. Besides H. pylori, several other gastric non-Helicobacter pylori Helicobacters (NHPH), mainly associated with pigs (H. suis) and pets (H. felis, H. bizzozeronii, H. salomonis, and H. heilmannii), may also colonize the human stomach and cause gastric disease, including gastritis, peptic ulcers and mucosa-associated lymphoid tissue (MALT) lymphoma. These NHPH lack homologous to the major known adhesins involved in colonization of the human stomach. In humans, NHPH infection rate is much lower than in the natural hosts. Differences in the glycosylation profile between gastric human and animal mucins acting as glycan receptors for NHPH-associated adhesins, may be involved. The identification and characterization of the key molecules involved in the adhesion of gastric Helicobacter species to the gastric mucosa is important to understand the colonization and infection strategies displayed by different members of this genus.

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“…Correct species identification is also important to better estimate the true prevalence of NHPH infections in humans, to allow identification of the source of infection (e.g., in pets), and hence, allow implementation of correct measurements to avoid reinfection (e.g., treatment of infected pets). Finally, as antimicrobial resistance in NHPHs has already been described and since antimicrobial susceptibility can differ between Helicobacter species, species identification may guide antimicrobial therapy as well [ 17 , 18 ]. So far, however, no ideal diagnostic tool exists to identify Helicobacter infections to the species level.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Gastric Helicobacter Species Using MALDI-TOF Mass Spectrometry

et al. 2021

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Gastric helicobacters (Helicobacter (H.) pylori and non-H. pylori Helicobacter species (NHPHs)) colonize the stomach of humans and/or animals. Helicobacter species identification is essential since many of them are recognized as human and/or animal pathogens. Currently, Helicobacter species can only be differentiated using molecular methods. Differentiation between NHPHs using MALDI-TOF MS has not been described before, probably because these species are poorly represented in current MALDI-TOF MS databases. Therefore, we identified 93 gastric Helicobacter isolates of 10 different Helicobacter species using MALDI-TOF MS in order to establish a more elaborate Helicobacter reference database. While the MALDI Biotyper database was not able to correctly identify any of the isolates, the in-house database correctly identified all individual mass spectra and resulted in 82% correct species identification based on the two highest log score matches (with log scores ≥2). In addition, a dendrogram was constructed using all newly created main spectrum profiles. Nine main clusters were formed, with some phylogenetically closely related Helicobacter species clustering closely together and well-defined subclusters being observed in specific species. Current results suggest that MALDI-TOF MS allows rapid differentiation between gastric Helicobacter species, provided that an extensive database is at hand and variation due to growth conditions and agar-medium-related peaks are taken into account.

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Antimicrobial Susceptibility Pattern of Helicobacter heilmannii and Helicobacter ailurogastricus Isolates

Cited by 15 publications

References 49 publications

Helicobacterspecies binding to the human gastric mucosa

Helicobacterspecies binding to the human gastric mucosa

Adhesion of Helicobacter Species to the Human Gastric Mucosa: A Deep Look Into Glycans Role

Differentiation of Gastric Helicobacter Species Using MALDI-TOF Mass Spectrometry

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