Antimicrobial susceptibility of Streptococcus species isolated from clinical mastitis in dairy cows

Guérin-Faublée, Véronique; Tardy, Florence; Bouveron, Clarisse; Carret, G.

doi:10.1016/s0924-8579(01)00485-x

Cited by 71 publications

(93 citation statements)

References 17 publications

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“…In the present work, consistent with such investigations (GUÉRIN-FAUBLÉE et al, 2002;MINST et al, 2012;RATO et al, 2013), most isolates (96.6%) were susceptible to penicillin, indicating that this antibiotic is a viable option for the treatment of mastitis. In humans, penicillin is the recommended first-line treatment for S. agalactiae infections for all age groups, in addition to being administered prophylactically to pregnant women (BOLUKAOTO et al, 2015;VERANI et al, 2010).…”

Section: Discussionsupporting

confidence: 90%

“…Erythromycin resistance has been documented worldwide in studies concerning S. agalactiae strains of human and bovine origin. In the United States and Europe, resistance rates of approximately 4-50% have been reported (DIPERSIO; DIPERSIO, 2006;DOGAN et al, 2005;GUÉRIN-FAUBLÉE et al, 2002;SADOWY et al, 2010), while in Brazil, estimates have been lower than 10% (CORRÊA et al, 2011;DUARTE et al, 2004DUARTE et al, , 2005PALMEIRO et al, 2010). However, of the Brazilian bovine isolates examined by Pinto et al (2013), 27.6% were resistant to this antibiotic, a higher value than that recorded in the present work (19.5%).…”

Section: Resultsmentioning

confidence: 99%

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In vitro antimicrobial susceptibility and genetic resistance determinants of Streptococcus agalactiae isolated from mastitic cows in Brazilian dairy herds

Silva

Castro

Gonçalves

et al. 2017

SCA

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Streptococcus agalactiae is one of the main causative agents of bovine mastitis and is associated with several economic losses for producers. Few studies have evaluated antimicrobial susceptibility and the prevalence of genetic resistance determinants among isolates of this bacterium from Brazilian dairy cattle. This work aimed to evaluate the frequency of the antimicrobial resistance genes ermA, ermB, mefA, tetO, tetM, aphA3, and aad-6, and in vitro susceptibility to the antimicrobials amikacin, erythromycin, clindamycin, tetracycline, gentamicin, penicillin, ceftiofur, and cefalotin, and the associations between resistance genotypes and phenotypes among 118 S. agalactiae isolates obtained from mastitic cows in Brazilian dairy herds. Of the resistance genes examined, ermB was found in 19 isolates (16.1%), tetO in 23 (19.5%), and tetM in 24 (20.3%). The genes ermA, mefA, aphA3, and aad-6 were not identified. There was an association between the presence of genes ermB, tetM, and tetO and phenotypic resistance to erythromycin, clindamycin, and tetracycline. Rates of resistance to the tested antibiotics varied, as follows: erythromycin (19.5%), tetracycline (35.6%), gentamicin (9.3%), clindamycin (20.3%), penicillin (3.4%), and amikacin (38.1%); conversely, all isolates were susceptible to ceftiofur and cefalotin. Antimicrobial resistance testing facilitates the treatment decision process, allowing the most judicious choice of antibiotics. Moreover, it enables regional and temporal monitoring of the resistance dynamics of this pathogen of high importance to human and animal health. Key words: Antimicrobial resistance genes. Bovine diseases. Bovine mastitis. GBS, MIC. ResumoStreptococcus agalactiae é um dos principais agentes causadores de mastite em bovinos e de consequentes perdas econômicas aos produtores. Poucos estudos que avaliaram a susceptibilidade a antimicrobianos e a presença de determinantes genéticos de resistência para este agente em bovinos leiteiros do Brasil. Este trabalho teve por objetivo avaliar a frequência dos genes de resistência a antimicrobianos ermA, ermB, mefA, tetO, tetM, aphA3, aad-6, bem como a susceptibilidade in vitro aos antimicrobianos amicacina, eritromicina, clindamicina, tetraciclina, gentamicina, penicilina, ceftiofur e cefalotina, e as associações entre genótipos e fenótipos de resistência em 118 isolados de S. agalactiae provenientes de casos de mastite em rebanhos bovinos brasileiros. Dentre os genes de resistência pesquisados, ermB foi encontrado em 19 isolados (16,1%), tetO em 23 (19,5%) e tetM em 24 (20,3%). Os genes ermA, mefA, apha3 e aad6 não foram detectados. Verificou-se associação entre a presença dos genes ermB, tetM e tetO e os fenótipos de resistência à eritromicina, clindamicina e tetraciclina. Foram encontrados diferentes índices de resistência aos antibióticos testados: Eritromicina (19,5%), tetraciclina (35,6%), gentamicina (9,3%), clindamicina (20,3%), penicilina (3,4%) e amicacina (38,1%). Todos os isolados foram susceptíveis ceftiofur e cefalotina. ...

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Section: Discussionsupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

In vitro antimicrobial susceptibility and genetic resistance determinants of Streptococcus agalactiae isolated from mastitic cows in Brazilian dairy herds

Silva

Castro

Gonçalves

et al. 2017

SCA

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“…Streptococcus agalactiae was the main pathogenic bacteria and resistance of strain also was high. Decreased susceptibility to penicillin has been reported previously (Guérin-Faublée et al, 2002;Rossitto et al, 2002). In contrast to the rate of penicillin resistance (approximately 10%) reported (Nam et al, 2009), the high rate of Streptococci in the present study was resistant to penicillin in the area.…”

Section: Discussioncontrasting

confidence: 74%

“…Streptococcus agalactiae is associated with cow and well adapted to the mammary gland, whereas Streptococcus dysgalactiae and Streptococcus uberis are environmental pathogens (Guérin-Faublée et al, 2002). Various species of Streptococcus are known to be associated with infections of cattle, pigs, horses, sheep, birds, aquatic mammals, and fishes.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance and virulence-related genes ofStreptococcusobtained from dairy cows with mastitis in Inner Mongolia, China

Ding

Zhao

et al. 2015

Pharmaceutical Biology

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Context: Mastitis is the most expensive disease in the dairy cattle industry and results in decreased reproductive performance. Streptococcus, especially Streptococcus agalactiae, possesses a variety of virulence factors that contribute to pathogenicity. Objective: Streptococcus isolated from mastitis was tested to assess the prevalence of antimicrobial resistance and distribution of antibiotic resistance-and virulence-related genes. Materials and methods: Eighty-one Streptococcus isolates were phenotypically characterized for antimicrobial resistance against 15 antibiotics by determining minimum inhibitory concentrations (MIC) using a micro-dilution method. Resistance-and virulence-related genes were detected by PCR. Results: High percentage of resistance to b-lactams, along with tetracycline and erythromycin, was found. Resistance to three or more of seven antimicrobial agents was observed at 88.9%, with penicillin-tetracycline-erythromycin-clindamycin as the major profile in Streptococcus isolates. Resistant genes were detected by PCR, the result showed that 86.4, 86.4, 81.5, and 38.3% of isolates were mainly carrying the pbp2b, tetL, tetM, and ermB genes, respectively. Nine virulence genes were investigated. Genes cyl, glnA, cfb, hylB, and scaA were found to be in 50% of isolates, while 3.7, 21, and 4.9% of isolates were positive for bca, lmb, and scpB, genes, respectively. None of the isolates carried the bac gene. Discussion and conclusion: This study suggests the need for prudent use of antimicrobial agents in veterinary clinical medicine to avoid the increase and dissemination of antimicrobial resistance arising from the use of antimicrobial drugs in animals.

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“…Additionally, the impact of this disease extends to the financial cost of therapeutic strategies and laboratory and veterinary services involved in control programs (15,16). The availability of information about the prevalence and the biological and epidemiological characteristics of the etiologic agents is essential for developing appropriate prevention programs and successful therapy (16,20).Streptococcus agalactiae, or group B Streptococcus (GBS), is a well-recognized worldwide etiological agent of mastitis in bovines, causing both clinical and mild subclinical mastitis of long duration (10,20). Bacterial cells are shed in milk from infected quarters, and transmission to uninfected quarters and cows usually occurs during the milking period (15, 21).…”

mentioning

confidence: 99%

Phenotypic and Molecular Characteristics of Streptococcus agalactiae Isolates Recovered from Milk of Dairy Cows in Brazil

et al. 2004

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Information on the characteristics of Streptococcus agalactiae obtained from bovine sources in Brazil is still very limited. The aim of this study was to assess the phenotypic and genotypic diversity among S. agalactiae isolates from milk of dairy cows presenting clinical or subclinical mastitis in the southeast region of Brazil. Phenotypic characterization was based on physiological and serological tests. Antimicrobial susceptibility tests were carried out by the disk method. Genetic diversity was evaluated by using random amplified polymorphic DNA-PCR (RAPD-PCR) (by using the primer 1254) and pulsed-field gel electrophoresis (PFGE) (by using SmaI as the restriction enzyme) and by PCRs for detection of genes associated with resistance to erythromycin and tetracycline as well as PCRs for detection of genes coding for cell surface-associated proteins. According to the results of physiologic tests, 45 (52.9%) isolates showed beta-hemolysis and 44 (51.7%) were susceptible to bacitracin. Fourteen different biotypes were detected. The two most frequent biotypes comprised strains that were non-beta-hemolytic; fermented galactose, lactose, and salicin; produced protease; and were negative for DNase production. Serotype III was predominant (66 isolates [77.6%]), followed by serotypes II, Ia, Ib, and VI. Resistance to tetracycline and erythromycin was found in 38 (44.7%) and 9 (10.5%) isolates, respectively, with tet(O) (31.7%) and erm(B) (100%) being the most frequently occurring resistance genes. Three genes coding for surface proteins, bca, lmb, and scpB, were detected in 55 (64.7%), 7 (8.2%), and 43 (50.5%) isolates, respectively. In most cases, isolates from animals in the same herd presented closely related genetic profiles (determined by either RAPD-PCR or PFGE), which were distinct from those of isolates from different herds.Infectious mastitis is an important health problem affecting dairy cattle and constitutes a source of economic loss for the dairy industry due to the effects on milk quality and yield. Additionally, the impact of this disease extends to the financial cost of therapeutic strategies and laboratory and veterinary services involved in control programs (15,16). The availability of information about the prevalence and the biological and epidemiological characteristics of the etiologic agents is essential for developing appropriate prevention programs and successful therapy (16,20).Streptococcus agalactiae, or group B Streptococcus (GBS), is a well-recognized worldwide etiological agent of mastitis in bovines, causing both clinical and mild subclinical mastitis of long duration (10,20). Bacterial cells are shed in milk from infected quarters, and transmission to uninfected quarters and cows usually occurs during the milking period (15, 21).The prevalence of GBS in cattle has been investigated in many areas, and the highest frequencies have been found in regions where appropriate control measures have not been implemented (20). In Brazil, studies have been developed by Empresa Brasileira de Agropecuá...

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Antimicrobial susceptibility of Streptococcus species isolated from clinical mastitis in dairy cows

Cited by 71 publications

References 17 publications

In vitro antimicrobial susceptibility and genetic resistance determinants of Streptococcus agalactiae isolated from mastitic cows in Brazilian dairy herds

In vitro antimicrobial susceptibility and genetic resistance determinants of Streptococcus agalactiae isolated from mastitic cows in Brazilian dairy herds

Antimicrobial resistance and virulence-related genes ofStreptococcusobtained from dairy cows with mastitis in Inner Mongolia, China

Phenotypic and Molecular Characteristics of Streptococcus agalactiae Isolates Recovered from Milk of Dairy Cows in Brazil

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