Antimicrobial Susceptibility of Staphylococcus aureus from Retail Ground Meats

Kelman, Alina; Soong, Yee-Ann; Dupuy, Nicole; Shafer, Daniel; Richbourg, William; Johnson, Kristy; Brown, Twain; Kestler, Edward; Li, Yi; Zheng, Jie; McDermott, Patrick F.; Meng, Jianghong

doi:10.4315/0362-028x.jfp-10-571

Cited by 44 publications

(50 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using data from the National Antibiotic Resistance Monitoring System retail meat program, Zhao et al (2012) found higher rates of E. coli contamination in a sample of 2988 chicken breasts and 2991 ground beef items. Three recent studies have reported on S. aureus and drug resistant S. aureus in the food supply (Hanson et al, 2011;Kelman et al, 2011;Waters et al, 2011). We found similar prevalence of S. aureus as (Kelman et al, 2011;Waters et al, 2011) and higher prevalence than Hanson et al (2011), a study that did not exclusively focus on ground beef.…”

Section: Discussionsupporting

confidence: 65%

“…Three recent studies have reported on S. aureus and drug resistant S. aureus in the food supply (Hanson et al, 2011;Kelman et al, 2011;Waters et al, 2011). We found similar prevalence of S. aureus as (Kelman et al, 2011;Waters et al, 2011) and higher prevalence than Hanson et al (2011), a study that did not exclusively focus on ground beef. Similar to Waters et al (2011) we found no confirmed MRSA.…”

Section: Discussionsupporting

confidence: 65%

“…This method is used by FDA in its surveys of bacterial contamination of retail meats (Zhao et al, 2012). S. aureus (including MRSA) is an increasingly documented food borne disease risk reported to be carried by both beef and poultry products (Hanson et al, 2011;Kelman et al, 2011;Waters et al, 2011).…”

Section: Microbiological Analysesmentioning

confidence: 99%

See 2 more Smart Citations

Food Safety and Food Access: A Pilot Study

Silbergeld¹,

Frisancho²,

Gittelsohn³

et al. 2013

JFR

View full text Add to dashboard Cite

(WC 238)Objectives: This study was designed to evaluate the feasibility of testing the hypothesis that differences in neighborhood level food access may be associated with consumer exposure to food borne microbial contamination. Methods:This study was carried out in Baltimore MD in 2011 among selected neighborhoods defined as high or low food access. In each category, packages of chicken thighs and ground beef were purchased from small stores and supermarkets. We evaluated presence of E. coli and Staphylococcus aureus and also tested isolates for antimicrobial resistance.Results: Microbial contamination of both chicken and beef products was highly prevalent (S. aureus-13/32 for chicken and 14/32 for beef; E. coli 21/32 for chicken and 12/32 for beef). Small stores were more likely to sell food carrying these microbes as well as MDR strains of both E. coli and S. aureus, and chicken was more likely to carry E. coli as compared to ground beef. Conclusions:This is the first study of this hypothesis. While it is limited in size and in focus on one US city, the results indicate that further research is appropriate to examine neighborhood level risk factors for differential exposures to food borne microbes. Abbreviations: S. aureus (Staphylococcus aureus); E. coli (Escherichia coli), MRSA (methicilllin resistantStaphylococcus aureus), MDR (multi-drug resistant), LFA (low food access), HFA (high food access). FDA (US Food and Drug Administration), USDA (US Department of Agriculture), CLSI (Clinical and Laboratory Standards Institute).

show abstract

Section: Discussionsupporting

confidence: 65%

Section: Discussionsupporting

confidence: 65%

See 1 more Smart Citation

Food Safety and Food Access: A Pilot Study

Silbergeld¹,

Frisancho²,

Gittelsohn³

et al. 2013

JFR

View full text Add to dashboard Cite

show abstract

“…Besides, antimicrobial resistance is a major public health concern worldwide, due to the persistent circulation of resistant strains of bacteria in the environment and the possible contamination of food. S. aureus has been reported to frequently show multiple antimicrobial resistance patterns (10,18,32).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterisation of Staphylococcus aureus strains isolated from beef, sheep and chicken meat

KEYVAN¹

2016

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

Summary:The objective of this study was to evaluate the occurrence, enterotoxigenic properties and antibiotic resistance of Staphylococcus aureus in beef, sheep and chicken meat. For this purpose, 225 meat samples were obtained from different supermarkets in Ankara. Firstly, these samples were analysed phenotypically for coagulase-positive staphylococci and S. aureus. Then phenotypically determined S. aureus isolates were confirmed through PCR assay and investigated by multiplex PCR for enterotoxin genes and mecA gene. According to the analyses, 14.6%, 30.6% and 45.3% of the beef, sheep and chicken meat samples respectively were found to be contaminated with S. aureus. Eighty eight (77.1%) out of 114 S. aureus isolates were determined as enterotoxigenic. Also, isolates contained newly described enterotoxin like genes. These results indicated that new types enterotoxin genes could be effective in establishing food intoxication. In this study, isolates were found resistant to one or more antibiotics. None of the S. aureus isolates contain mecA gene but some of them were identified as resistant to a group of antibiotics like oxacillin and cefoxitin, which are used in determining methicillin resistance. For public health and hygienic meat production HACCP and GMP systems should be implemented effectively.Keywords: Enterotoxin, meat, mecA, PCR, Staphylococcus aureus. Sığır, koyun ve piliç etlerinden Staphylococcus aureus'un izolasyon ve karakterizasyonuÖzet: Bu çalışma sığır, koyun ve piliç etlerinde Staphylococcus aureus'un bulunuşu, enterotoksijenik özellikleri ve antibiyotik dirençliliklerini saptamak amacıyla yapılmıştır. Bu amaçla, Ankara'daki farklı süpermarketlerden 225 adet et örneği alınmıştır. Alınan örnekler öncelikle koagulaz-pozitif stafilokoklar ve S. aureus yönünden analiz edilmiştir. Fenotipik testler sonucu S. aureus olarak belirlenen suşlar PCR tekniği ile doğrulanarak, multipleks PCR tekniği kullanılarak enterotoksin genleri ve mecA geni yönünden analiz edilmiştir. Analiz sonuçlarına göre sığır, koyun ve piliç eti örnekleri S. aureus ile sırasıyla % 14.6, % 30.6 ve % 45.3 düzeyinde kontamine bulunmuştur. İzole edilen 114 adet S. aureus izolatından 88'inin (% 77.1) enterotoksijenik özellikte olduğu tespit edilmiştir. İzolatların yeni tanımlanan enterotoksin genlerine sahip oldukları belirlenmiştir. Sonuçlar, yeni tanımlanan enterotoksin genlerinin gıda intoksikasyonlarında önemli olabileceğini ortaya koymaktadır. Bu çalışmada izolatlar bir veya daha fazla antibiyotiğe dirençli bulunmuştur. S. aureus izolatlarının hiçbirinde mecA geni saptanmamasına karşın, bazı izolatlar metisilin duyarlılığının belirlenmesinde kullanılan, oxacillin ve cefoxitine dirençli bulunmuştur. Halk sağlığının korunması ve hijyenik et üretimi için HACCP ve GMP sistemlerinin etkin uygulanması önemlidir.

show abstract

“…Manual handling during processing also can facilitate the entry of human MRSA strains into the production units. Recent surveillance data suggest that 22.5-64.8% of retail beef, pork, chicken, and turkey meats in ive diferent geographical locations in the United States were contaminated with S. aureus [148][149][150]. A Swiss meat-processing plant reported the presence of S. aureus on 22.7% of the received chilled pork hind quarters from 18 European suppliers [151].…”

Section: Mrsa In Food Processing Environmentmentioning

confidence: 99%

Methicillin-Resistant Staphylococcus aureus (MRSA) in Food- Producing and Companion Animals and Food Products

Chon¹,

Sung²,

Khan³

2017

Frontiers in Staphylococcus Aureus

View full text Add to dashboard Cite

Methicillin-resistant Staphylococcus aureus (MRSA) has become a growing concern in companion and food-producing animals. The presence of multidrug-resistance with a wide range of extracellular enterotoxin genes, virulence factors, and Panton-Valentine leukocidin (pvl) cytotoxin genes confer life-threatening traits on MRSA and makes them highly pathogenic and diicult to treat. Clonal complex 398 (CC398), a predominant clonal lineage of livestock-associated-MRSA in domestic animals and retail meat, is capable of infecting humans. In order to monitor and prevent MRSA contamination, it is critical to understand its source and transmission dynamics. In this review, we describe MRSA in food-producing animals (pig, catle, chicken), horses, pet animals (dogs, cats), and food products (pork, beef, chicken, milk, and ish).

show abstract

Antimicrobial Susceptibility of Staphylococcus aureus from Retail Ground Meats

Cited by 44 publications

References 28 publications

Food Safety and Food Access: A Pilot Study

Food Safety and Food Access: A Pilot Study

Isolation and characterisation of Staphylococcus aureus strains isolated from beef, sheep and chicken meat

Methicillin-Resistant Staphylococcus aureus (MRSA) in Food- Producing and Companion Animals and Food Products

Contact Info

Product

Resources

About