Antimicrobial susceptibility of pathogenic bacteria isolated from swine lungs

Pascu, C.; Herman, V.; Costinar, L.; Iancu, I.

doi:10.25083/rbl/24.3/506.512

Cited by 3 publications

(2 citation statements)

References 11 publications

(12 reference statements)

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“…None of the isolates were sensitive for all antimicrobials, an aspect also observed by others [ 34 , 35 , 36 ]. In the present research, all 14 strains tested showed the multiple resistance phenomenon, as follows: six strains (42.9%) to five antimicrobials, three strains (21.4%) to six antimicrobials, two strains (14.3%) to eight antimicrobials, two strains (14.3%) to nine antimicrobials and one strain (7.1%) to ten antimicrobials.…”

Section: Resultssupporting

confidence: 78%

Etiology of Mastitis and Antimicrobial Resistance in Dairy Cattle Farms in the Western Part of Romania

et al. 2022

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The present study aimed to determine the bacteria isolated from bovine mastitis and their antimicrobial resistance in the western part of Romania. Clinical mastitis was diagnosed based on local inflammation in the udder, changes in milk, and when present, generalized symptoms. Subclinical mastitis was assessed using a rapid test—the California Mastitis Test. The identification of bacterial strains was performed based on biochemical profiles using API system tests (API 20 E, API Staph, API 20 Strep, API Coryne, API 20 NE (bioMerieux, Marcy l’Etoile, France), and MALDI-TOF mass spectrometry (MS). The prevalent isolated bacteria were Staphylococcus spp. (50/116; 43.19%), followed by Streptococcus spp. (26/116; 22.41%), E. coli (16/116; 13.79%), Corynebacterium spp. (9/116; 7.75%), Enterococcus spp. (10/116; 8.62%), and Enterobacter spp. (5/116; 4.31%). Phenotype antimicrobial resistance profiling was performed used the disc diffusion method. Generally, Gram-positive bacteria showed low susceptibility to most of the antimicrobials tested, except cephalothin. Susceptibilities to penicillins and quinolones were fairly high in Gram-negative bacteria, whereas resistance was observed to macrolides, aminoglycosides, and tetracyclines. The highest number of isolates were multidrug resistant (MDR), the resistance pathotypes identified including the most frequently antimicrobials used in cow mastitis treatment in Romania.

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Section: Resultssupporting

confidence: 78%

Etiology of Mastitis and Antimicrobial Resistance in Dairy Cattle Farms in the Western Part of Romania

et al. 2022

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“…For this reason, we consider that it is particularly important to have a high degree of hygiene among the staff, equipment, and laboratory where the semen is processed. The production of semen doses with low bacterial contamination and high viability of sperm will be possible only with a strict hygienic control in the processing of semen and having the antibiotic resistance profile of bacteria that can contaminate semen [ 12 , 34 , 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Boar Semen Contamination: Identification of Gram-Negative Bacteria and Antimicrobial Resistance Profile

Costinar

Herman

Pitoiu³

et al. 2021

Animals

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Bacterial contamination of boar semen occurs with some frequency in artificial insemination centers and may have a negative effect on the quality of the semen as well as on the sows’ reproductive capacity. Normally, the source of bacterial contamination in pig seminal doses is the own boar. However, distilled water or laboratory equipment used to elaborate the seminal doses can be an important source of bacterial contamination. This study focused on the identification of gram-negative bacteria in boar semen, and impact on the quality of ejaculates obtained from boar, as well as on the establishment of antimicrobial resistance patterns of isolated gram-negative bacteria. Semen samples were collected from 96 boars, ranging in age from 12–36 month, from three artificial insemination centers from the North-West of Romania. Bacterial species were identified by two methods: matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and API 20 E (BioMerieux, France). The main bacteria isolated from the doses diluted semen were gram-negative bacteria (47.91%), with a majority of the contaminant bacteria belonging to the family Enterobacteriaceae: Seratia marcescens 19.56%, Proteus mirabilis 15.21% and Escherichia coli 10.86% and to the family Pseudomonaceae: Ralstonia picketii 17.39%, Burkholderia cepacia 10.86%, Pseudomonas aeruginosa 8.69%, and Pseudomonas fluorescens 4.34%, respectively. More than half of these isolates (56.52%) were resistant to gentamycin and 58.69% were resistant to penicillin. These antibiotics are very frequently added in sperm diluent in the centers for the processing of sperm from boars in Romania. Regular monitoring for bacterial contamination is an important aspect of a control program.

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Identification of Intestinal Bacterial Flora Isolated From Jackals in Western Romania

Costinar,

Herman,

Pascu

2023

SGEM International Multidisciplinary Scientific GeoConference� EXPO Proceedings

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The golden jackal (Canis aureus), a mesocarnivore, is currently spreading from eastern to western Europe. In Europe, the golden jackal is considered an invasive species, as in recent years it has managed to populate extensive territories in Greece, Turkey, Slovenia, Serbia and Hungary. Bulgaria is considered the country with the largest population of jackals in Europe. In Romania it is frequently found from the plains of Dobrogea to Banat. The present work aimed to study and observe the newly developed antibiotic resistance profiles of intestinal bacterial flora isolated from these wild species and to compare our data with those existing in the literature. Duodenum samples from golden jackals were collected for primary bacteriological examinations. This study used common identification and isolation methods and modern identification techniques, such as proteomics and MALDI-TOF MS technique, for highly accurate information on the bacterial flora of the digestive system. Antibiograms were performed using the disc-diffusimetric method using antibiotics and chemotherapeutics belonging to several groups. Several bacterial species were isolated from the jackals studied: Serratia liquefaciens, Staphylococcus hominis and Aeromonas salmonicida. In Aeromonas salmonicida strains, antibiograms showed significant percentages of intermediate strains to most of the antimicrobials used and overall resistance to levofloxacin. The same behaviour was recorded for colistin and strains belonging to the Seratia and Streptococcus genera. Interestingly, some bacterial strains isolated showed resistance to levofloxacin, a third- generation fluoroquinolone, as this antimicrobial is banned for veterinary use in EU countries. The results are a wake-up call, as antibiotic resistance has been shown to be present in wild animal populations in Romania, with risk to public health.

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Antimicrobial susceptibility of pathogenic bacteria isolated from swine lungs

Cited by 3 publications

References 11 publications

Etiology of Mastitis and Antimicrobial Resistance in Dairy Cattle Farms in the Western Part of Romania

Etiology of Mastitis and Antimicrobial Resistance in Dairy Cattle Farms in the Western Part of Romania

Boar Semen Contamination: Identification of Gram-Negative Bacteria and Antimicrobial Resistance Profile

Identification of Intestinal Bacterial Flora Isolated From Jackals in Western Romania

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