Etiology of Mastitis and Antimicrobial Resistance in Dairy Cattle Farms in the Western Part of Romania

Pascu, C.; Herman, V.; Iancu, I.; Costinar, L.

doi:10.3390/antibiotics11010057

Cited by 52 publications

(41 citation statements)

References 57 publications

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“…Antimicrobial treatment of mastitis is anticipated to become problematic in the near future due to the rapid increase in antibiotic-resistant pathogens [ 39 , 40 ]. For this reason, there is a growing need to identify and use alternatives to antibiotics such as natural formulations.…”

Section: Discussionmentioning

confidence: 99%

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Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils

et al. 2022

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Mastitis is considered to be one of the most important diseases of dairy cows in terms of health, production, and economy. Being the most common cause of antibiotic consumption in dairy cows, treatment of this disease is one of the biggest challenges in the veterinary profession as an increasing number of pathogens develop resistance to antibiotics used in the treatment. Therefore, new alternative approaches for limiting the use of antibiotics in livestock are required. For this reason, our study aimed to investigate prevalence of environmental mastitis associated bacterial strains, as well as the sensitivity of isolated strains to different antibiotics. Additionally, the therapeutic potential of three essential oils (EOs) was tested against bovine Serratia spp. and Proteus spp. mastitis pathogens, based on their chemical composition, as well as antibacterial potential. The study was carried out on 81 milk samples collected from dairy cows with mastitis. In order to determine prevalence of S. marcescens and P. mirabilis, microbiological isolation and identification were performed. Antimicrobial susceptibility testing was performed by disk diffusion method and the microdilution method was used to determine the antibacterial activity of selected EOs. In the oregano EO, a total of 23 compounds were detected, with carvacrol as a dominant component (78.94%). A total of 26 components were present in the EO of common thyme, where thymol was the most abundant compound (46.37%). Thymol also dominated (55.11%) the wild thyme EO. All tested EOs displayed antibacterial activity against all strains to different extents, while wild and common thyme EOs were the most effective. It could be concluded that the tested EOs represent promising therapeutic candidates for effective non-antibiotic treatment of mastitis.

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Section: Discussionmentioning

confidence: 99%

“…Antimicrobials are an important part of mastitis therapy [ 40 , 46 , 47 ]. Determination of the antimicrobial susceptibility pattern is necessary to choose the appropriate antimicrobial treatment for cows [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils

et al. 2022

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“…Klebsiella oxytoca, Enterobacter cloacae como los patógenos más frecuentes causantes de mastitis subclínica en las condiciones en el que se desarrolla la crianza en los cuatro establos estudiados. Estas bacterias son reconocidos como patógenos dentro del grupo de coliformes(Cobirka et al, 2020;Zaatout et al, 2020), relacionados con la contaminación fecal y vinculados principalmente con la mastitis clínica como lo describenPascu et al (2022). En el caso de Streptococcus agalactiae, se coincide con los reportesPascu et al (2022) quienes encuentran que este patógeno es un hallazgo frecuentes en casos de mastitis en bovinos con presentación entre 6,8 y 14,4%.La resistencia antibiótica es un problema creciente en casos enfermedades infecciosas en el ganado (incluyendo la mastitis), en la salud animal en general y la salud publica en el mundo(Pascu et al., 2022).…”

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“…Estas bacterias son reconocidos como patógenos dentro del grupo de coliformes(Cobirka et al, 2020;Zaatout et al, 2020), relacionados con la contaminación fecal y vinculados principalmente con la mastitis clínica como lo describenPascu et al (2022). En el caso de Streptococcus agalactiae, se coincide con los reportesPascu et al (2022) quienes encuentran que este patógeno es un hallazgo frecuentes en casos de mastitis en bovinos con presentación entre 6,8 y 14,4%.La resistencia antibiótica es un problema creciente en casos enfermedades infecciosas en el ganado (incluyendo la mastitis), en la salud animal en general y la salud publica en el mundo(Pascu et al., 2022). Los resultados del estudio muestran niveles de resistencia hasta de 100% de algunas patógenos a determinados antibióticos o niveles cercanos a estos.…”

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Evaluación de la resistencia antibiótica de bacterias aisladas de mastitis subclínica en bovinos de establos lecheros de Lurín, Lima

Quezada¹,

Morales-Cauti²

2022

Salud tecnol. vet.

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El objetivo del estudio fue determinar la resistencia antibiótica de bacterias aisladas de cuadros de mastitis subclínica en cuatro establos lecheros del distrito de Lurín, Lima - Perú, el año 2017. Se evaluaron 586 vacas Holstein sin cuadros de mastitis clínica durante la estación de verano, mediante la prueba de California Mastitis (CMT). Se colectaron muestras de leche de los cuartos que resultaron positivas al CMT (+, ++, +++) y se procedió al cultivo bacteriológico (agar sangre y agar McConkey) y se realizaron pruebas bioquímicas. Se evaluó la resistencia microbiana a ocho antibióticos utilizando la prueba de sensibilidad Kirby-Bauer. Se encontró 43.7% (256/586) de vacas con mastitis subclínicas y 12.4% (291/2344) de cuartos mamarios afectados. Las bacterias aisladas con mayor frecuencia fueron Klebsiella oxytoca (20.2%), Enterobacter cloacae (12.1%), y Streptococcus agalactiae (9.1%). Se observó resistencia a antibióticos en K. oxytoca a penicilina, cefalotina, amikacina, estreptomicina, tetraciclina, gentamicina, cefalexina y enrofloxacina entre un 62.9% y 91.9%. Las cepas de E. cloacae presentaron 100% de resistencia a la penicilina y para cefalotina, amikacina, estreptomicina, tetraciclina, gentamicina, cefalexina y enrofloxacina, entre 86.5% y 97.3%. En los aislados de S. agalactiae la resistencia observada a la penicilina, cefalotina, estreptomicina, tetraciclina, gentamicina, cefalexina y enrofloxacina estuvo entre el 10.7% y 82.1%. Para la amikacina la sensibilidad registrada fue del 100%.

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“…or positive for an SCC test in China. In a recent study,Pascu et al (2022) isolated Enterococcus spp. from 1Á72 to 6Á9% of milk samples from 127 cows (4 herds) with clinical and subclinical mastitis in Romania, respectively Bi et al (2016).…”

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confidence: 99%

Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows

Juliano

Gouvea

Latosinski

et al. 2022

Letters in Applied Microbiology

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The objectives were to study the diversity of Enterococcus spp. isolated from mastitis cases, milking equipment and the environment of dairy cows; and to determine in vitro resistance of isolates to antimicrobials that are relevant to human and animal health. Ten dairy farms were visited to collect samples from mastitis cases, faeces, bedding, aisles, water and milking equipment. Identification of Enterococcus at the species level and antimicrobial resistance testing was performed by MALDI‐TOF and the disk‐diffusion method, respectively. Of 365 isolates, Enterococcus hirae was the most prevalent, being more likely to be isolated from faeces than from milk (odds ratio (OR) = 39·2), liners (OR = 5·4) or bedding (OR = 2·2). Enterococcus saccharolyticus was the most prevalent in milk samples. The chances of isolating Enterococcus faecalis from milk were higher than from aisles (OR = 12·5), faeces (OR = 5·3), bedding (OR = 3·6) or liners (OR = 3·0). The odds of isolating Enterococcus faecium from faeces were higher than from liners (OR = 7·3), bedding (OR = 2·5) or aisles (OR = 2·4). Of 360 tested isolates, 1·9, 0·3 and 0·6% were resistant to penicillin, vancomycin and teicoplanin, respectively. Our results suggest that Enterococcus species can occupy specific ecological niches on dairy farms and pose a risk to public and animal health.

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Etiology of Mastitis and Antimicrobial Resistance in Dairy Cattle Farms in the Western Part of Romania

Cited by 52 publications

References 57 publications

Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils

Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils

Evaluación de la resistencia antibiótica de bacterias aisladas de mastitis subclínica en bovinos de establos lecheros de Lurín, Lima

Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows

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