Antimicrobial susceptibility of Actinobacillus pleuropneumoniae, Pasteurella multocida and Salmonella choleraesuis isolates from pigs

Abstract

The in vitro susceptibility of 839 isolates of Actinobacillus pleuropneumoniae, 969 isolates of Pasteurella multocida and 104 isolates of Salmonella choleraesuis from pigs to the fluoroquinolone danofloxacin, and eight other commonly used antimicrobial drugs was determined by veterinary diagnostic laboratories in Europe, Japan, South Africa and North America between 1989 and 1991, by using a broth microdilution technique. The minimum inhibitory concentrations of danofloxacin, amoxycillin, ceftiofur, erythromycin, gentamicin, lincomycin, oxytetracycline, spectinomycin and trimethoprim:sulphamethoxazole (ratio 1:19) that prevented the growth of 90 per cent of the bacteria were 0.125, < or = 0.5, < or = 0.125, 8, 8, 32, 32, 64 and < or = 0.25 microgram/ml for A pleuropneumoniae, 0.06, 1, < or = 0.125, 8, 4, 64, 8, 32 and 8 micrograms/ml for P multocida, and 0.125, > 64, < or = 1, > 64, 1, > 64, > 64, 64 and < or = 0.25 microgram/ml for S choleraesuis. These data confirm the high in vitro potency of danofloxacin against field isolates that show significant resistance to several other antibacterial drugs.

Antimicrobial Susceptibility of Actinobacillus pleuropneumoniae Isolated from Pigs in Korea Using New Standardized Procedures.

“…However, in Japan and other countries a relatively high number of penicllin-resistant isolates have been reported [2,5,11]. In this study, the in vivo activities of the ceftiofur and danofloxacin were in agreement with those of previous reports [2,10,11].…”

“…Current bacterins provide only serotype specific protection. Differences in antimicrobial susceptibility of A. pleuropneumoniae isolates from country to country were detected for antimicrobial agents when comparing minimal inhibitory concentration (MIC) data from one country with those from another [1,2,5,10,11]. For pleuropneumonia treatment to be effective, it is important to know the antimicrobial susceptibility of A. pleuropneumoniae isolates that are isolated in each country.…”

Antimicrobial Susceptibility of Pasteurella multocida Isolated From Cattle and Pigs

“…Antimicrobial therapy is the major effective method for treating animal pasteurellosis and a wide variety of antimicrobial agents has been clinically available. Antimicrobial susceptibility testing has been conducted world-wide, by determining minimum inhibitory concentrations (MICs), for either porcine isolates (Yamamoto et al, 1990;Iwamatsu et al, 1991;Gutie Ârrez Martin and Rodrõ Âguez Ferri, 1993;Raemdonck et al, 1994;Blackall et al, 1995;Salmon et al, 1995;Bousquet et al, 1997) or bovine isolates (Stephens et al, 1993;Watts et al, 1994). However, the MIC antimicrobial susceptibility data compared between bovine and porcine isolates are scant (Ho Èrmansdorfer and Bauer, 1998).…”

Protective Effect of Pasteurella multocida Cell-Free Antigen and Toxoid against Challenge with Toxigenic Strains of Pasteurella multocida in Mice

“…There have been an increasing number of pathogens resistant to antimicrobials on various farms recently [2,16,19], but no vaccine for pneumonia from P. multocida infection has yet been developed in Japan. To develop an effective vaccine for pneumonia from P. multocida infection, we examined the protective activities of the cell-free antigen (CFA) obtained from the culture supernatant of toxigenic P. multocida B-45 strain (type A; isolated from pneumonic lesions in a pig) and the toxin (PMT) purified from CFA for the infection with toxigenic strains of P. multocida B-45 and B-67 (type D; isolated from a nasal swab obtained from swine with symptoms of atrophic rhinitis) in mice.…”