Antimicrobial susceptibility of 6 antimicrobial agents in  Helicobacter pylori  clinical isolates by using EUCAST breakpoints compared with previously used breakpoints

Alarcón, Teresa; Urruzuno, Pedro; Martínez, María Josefa García; Domingo, Diego; Llorca, Laura; Corrêa, Ana Beatriz de Almeida; López-Brea, M

doi:10.1016/j.eimc.2016.02.010

Cited by 28 publications

(13 citation statements)

References 19 publications

(18 reference statements)

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“…Interestingly, the duality of resistance rates was observed in this article for amoxicillin due to discordant cutoff proposed by the literature for Latin America [45] and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) [47]. This difference demonstrated the lack of clear guidelines on monitoring studies for the treatment of H. pylori, showing that there is insufficient clinical and scientific evidence to delineate the cutoff points for amoxicillin, metronidazole, levofloxacin, and tetracycline in the eradication of H. pylori [48,49]. Larger studies are necessary to find the correlation of efficacy with the different levels of antibiotic susceptibility, mainly in Latin-America.…”

Section: Antibiotic Susceptibilitymentioning

confidence: 80%

Prevalence of Infection and Antibiotic Susceptibility of Helicobacter pylori: An Evaluation in Public and Private Health Systems of Southern Chile

et al. 2019

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Helicobacter pylori colonizes half of the human population. Age, ethnicity, and socioeconomic status are factors that influence the prevalence of the infection. This is important in southern Chile, one of the most unequal regions in the world, where a significant difference in the health access of the population occurs due to the existence of two competing health systems. Moreover, in the last few years, current protocols of H. pylori eradication have shown high rates of resistance with reduced therapeutic efficacy. This study reported the epidemiology of infection and attempted to identify divergent points among the population beneficiaries of the two health care schemes in southern Chile. Biopsies from public (n = 143) and private (n = 86) health systems were studied. At the same time, clinical and sociodemographic factors were evaluated. H. pylori strains were obtained from gastric biopsies for culture and molecular testing. Antibiotic susceptibility was determined by the agar dilution method. Differences about ethnicity, rural residence, and education (p ≤ 0.05) were observed between beneficiaries of the two health systems. The prevalence of H. pylori was 45%, with no significant differences regardless of the socioeconomic conditions. The only identified risk factor associated with H. pylori infection was Mapuche ethnicity (OR (odds ratio) = 2.30). H. pylori showed high resistance rates, particularly against clarithromycin (40%), levofloxacin (43.1%), and metronidazole (81.8%). This study highlighted the importance of Mapuche ancestry as a risk factor in southern Chile and emphasized the need to search for new eradication strategies as well as further studies evaluating therapeutic efficacy.

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Section: Antibiotic Susceptibilitymentioning

confidence: 80%

Prevalence of Infection and Antibiotic Susceptibility of Helicobacter pylori: An Evaluation in Public and Private Health Systems of Southern Chile

et al. 2019

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“…In Europe, metronidazole resistance has been estimated in the range of 30–40% [ 5 , 6 ] and this prevalence of resistance in H. pylori is even higher in developing countries ranging from 40.5% to 95.4% [ 50 ]. Metronidazole has been widely prescribed for infections such as parasitic or female genital infections and could contribute to the high resistance rate found everywhere [ 51 ]. Although rifampicin is not commonly used as a first treatment option in H. pylori infection, we observed a rising rate of resistance in comparison with previous reports [ 52 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, it is estimated that among antibiotics used for the treatment of H. pylori, resistance rates to amoxicillin and tetracycline are the lowest. The level of resistance to these antibiotics has been reported as very low (< 10%) or even absent in Europe [ 5 , 6 , 51 ]. In the present work, only one strain was resistant to amoxicillin ( Hp1 strain), and no resistant strains to tetracycline were detected.…”

Section: Discussionmentioning

confidence: 99%

Procyanidin-Rich Extract from Grape Seeds as a Putative Tool against Helicobacter pylori

Silván

Gutiérrez-Docio

Moreno-Fernández

et al. 2020

Foods

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Strains of Helicobacter pylori (H. pylori) resistant to various antibiotics have increased in recent years. In this context, the search for new therapeutic approaches is crucial. The aim of the present study was to demonstrate the antibacterial activity of a procyanidin-rich extract obtained from food-grade winery grape seeds against 14 H. pylori strains and elucidate its phenolic composition. Ten strains (71.4%) showed resistance to at least some of the tested antibiotics, while four isolates (28.6%) were susceptible to all antibiotics. Resistance to more than one class of antibiotics was observed in six strains (42.9%). The extract was able to inhibit the growth of all H. pylori strains in a range of a minimum inhibitory concentration (MIC) from 0.015 mg/mL to 0.125 mg/mL, confirming also the existence of a strain-dependent effect. The phenolic composition determined by reverse phase high pressure liquid chromatography, photodiode array, and mass spectrometry detection (RP-HPLC-PAD-MS) analysis revealed the presence of 43 individual compounds and allowed the quantification of 41 of them, including seven procyanidin tetramers, seven procyanidin pentamers, and six galloylated procyanidin dimers, trimers, and tetramers. The extract was composed mainly by catechin and procyanidin oligomers with a total amount of 5,801 mg/100 g, which represent 92% of the total individual phenolic content. Among them, the most abundant were catechins (2,047 mg/100 g), followed by procyanidin dimers (1,550 mg/100 g), trimers (1,176 mg/100 g), tetramers (436 mg/100 g), and pentamers (296 mg/100 g) that represent 35, 27, 20, 8, and 5%, respectively of the total flavanol constituents. The composition profile information may help to improve the production process of useful antibacterial extracts against H. pylori.

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“…The in vitro minimum inhibitory concentrations (MICs) of the five antibiotics against the H. pylori clinical isolates were defined by the points of intersection of the inhibitory zones with the strips. To determine susceptibility, we adopted the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines [ 19 ]. The MIC breakpoints for amoxicillin, clarithromycin, metronidazole, tetracycline, and levofloxacin were >0.125, >0.5, >8, >1, and >1 mg/L, respectively.…”

Section: Methodsmentioning

confidence: 99%

Is Only Clarithromycin Susceptibility Important for the Successful Eradication of Helicobacter pylori?

et al. 2020

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Resistance to clarithromycin and other antibiotics included in the eradication regimen, such as amoxicillin and metronidazole, is important for Helicobacter pylori (H. pylori) eradication. The aim of this study was to investigate the correlation between the results of antimicrobial susceptibility testing and the eradication rate, as well as to understand the importance of antimicrobial susceptibility testing in H. pylori eradication. We retrospectively reviewed the electronic medical records of 1862 patients who underwent gastric biopsy for the culture of H. pylori during upper endoscopy from March 2015 to June 2019. We tried to find a correlation between the results of the antimicrobial susceptibility testing and the eradication rate in patients who received standard triple or concomitant therapy. A total of 247 patients exhibited positive results for culture and underwent antimicrobial susceptibility testing. Of these, 146 received eradication therapy, with follow-up tests after treatment. In the standard triple therapy, patients who were susceptible to both clarithromycin and amoxicillin exhibited significantly higher eradication rates (85.9%) than those susceptible to clarithromycin and resistant to amoxicillin (75.0%) or those resistant to clarithromycin and susceptible to amoxicillin (44.4%) (p = 0.013). In the concomitant therapy, patients who were susceptible to both clarithromycin and metronidazole had significantly higher eradication rates (96.3%) than those susceptible to clarithromycin and resistant to metronidazole (88.9%) or those resistant to clarithromycin and susceptible to metronidazole (50.0%) (p = 0.016). There was a correlation between the results of antimicrobial susceptibility testing and the eradication rate for H. pylori. In addition to clarithromycin, susceptibility to amoxicillin and metronidazole is also important for the successful eradication of H. pylori.

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Antimicrobial susceptibility of 6 antimicrobial agents in Helicobacter pylori clinical isolates by using EUCAST breakpoints compared with previously used breakpoints

Cited by 28 publications

References 19 publications

Prevalence of Infection and Antibiotic Susceptibility of Helicobacter pylori: An Evaluation in Public and Private Health Systems of Southern Chile

Prevalence of Infection and Antibiotic Susceptibility of Helicobacter pylori: An Evaluation in Public and Private Health Systems of Southern Chile

Procyanidin-Rich Extract from Grape Seeds as a Putative Tool against Helicobacter pylori

Is Only Clarithromycin Susceptibility Important for the Successful Eradication of Helicobacter pylori?

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