Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of &amp;lt;i&amp;gt;Pseudomonas aeruginosa&amp;lt;/i&amp;gt; Strains Isolated from Clinical Samples

Zaranza, Alicia Valéria; Morais, Francyelle Costa; Carmo, Monique Santos do; Marques, Adriana de Mendonça; Monteiro, Cristina de Andrade; Ferro, Thiago Feitosa; Monteiro-Neto, Valério; Figueiredo, Patrícia de Maria Silva

doi:10.4236/jbnb.2013.41013

Cited by 14 publications

(5 citation statements)

References 31 publications

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“…Coproduction of AmpC and ESBL (3.9%) in the isolates of this study was similar to the findings of Upadhyay et al (3.3%), but much lower than several other reports (24.5-26%) ( 17 , 22 , 23 ). Finally, similar to a report from Brazil, the isolates of the present study did not harbor SHV and CTX-M genes ( 32 ).…”

Section: Discussionsupporting

confidence: 91%

Prevalence of Extended-Spectrum and Metallo β-Lactamase Production in AmpC β-Lactamase Producing Pseudomonas Aeruginosa Isolates From Burns

Rafiee

Eftekhar

Tabatabaei

et al. 2014

Jundishapur J Microbiol

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Background:Pseudomonas aeruginosa is one of the most common causes of nosocomial infections. Resistance of P. aeruginosa to β-lactam antibiotics may be the result of acquired resistance through mutation and over production of various antibiotic inactivating enzymes. This research aimed to determine the prevalence of extended-spectrum β-lactamases (ESBL) and metallo β-lactamase (MBL) production as well as the presence of their related genes among AmpC β-lactamase producing P. aeruginosa isolated from burns.Objectives:The current study aimed to determine the prevalence of class A ESBL and MBL production in relation to the presence of their related genes among AmpC β-lactamase producing P. aeruginosa isolated from burns.Materials and Methods:The antimicrobial susceptibility of 51 P. aeruginosa isolates from patients with burns was examined against 13 antibiotics by the disc diffusion method. Minimum inhibitory concentrations (MIC) for imipenem and ceftazidime were measured by the microdilution method. AmpC production was detected by AmpC disc and the modified three-dimensional extract tests. ESBL phenotype was confirmed by the double disc synergy test (DDST). Presence of β-lactamase genes was detected by specific primers and polymerase chain reaction (PCR).Results:All isolates were multidrug resistant. AmpC, ESBL and MBL production were observed in 35 (68.6%), 20 (39.2%) and 19 (37.3%) isolates, respectively. Overall, 43 isolates (84.3%) carried β-lactamase genes, out of which 31 (60.8%) harbored blaAmpC, 20 (39.2%) had blaTEM and 11 (21.6%) carried blaPER-1 genes. Among the AmpC producers, two isolates (6.5%) carried blaAmpC + blaESBL, 13 (41.9%) had blaAmpC + blaMBL and six (19.4%) produced the three enzymes.Conclusions:A high prevalence of multiple β-lactamase production was observed among the AmpC producers (60%), of which the majority co-produced AmpC and MBL. The current study results showed correlation between β-lactamase production and the presence of antibiotic resistance genes in the isolates.

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Section: Discussionsupporting

confidence: 91%

Prevalence of Extended-Spectrum and Metallo β-Lactamase Production in AmpC β-Lactamase Producing Pseudomonas Aeruginosa Isolates From Burns

Rafiee

Eftekhar

Tabatabaei

et al. 2014

Jundishapur J Microbiol

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“…In a study carried out by Banar et al [ 20 ], the frequency of these genes among 57 P. aeruginosa strains was as follows: algD (100%), pelF (93%), and pslD (54.6%). Also, in other studies conducted by Namuq et al, Ghadaksaz et al, and Zaranza et al, the frequency of the algD gene has been reported as 98%, 87.5%, and 39%, respectively [ 29 , 33 , 34 ]. Bacterial strains that carry the genes encoding the biofilm formation have the potential to cause severe infections.…”

Section: Discussionmentioning

confidence: 85%

Prevalence of algD, pslD, pelF, Ppgl, and PAPI-1 Genes Involved in Biofilm Formation in Clinical Pseudomonas aeruginosa Strains

Rajabi

Salimizand

Khodabandehloo

et al. 2022

BioMed Research International

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Introduction. Biofilm formation is one of the main virulence factors in Pseudomonas aeruginosa infections. This study is aimed at investigating the presence of genes involved in biofilm formation in clinical P. aeruginosa isolates. Material and Methods. A cross-sectional study was conducted on 112 P. aeruginosa isolates. The biofilm formation assay was performed on all isolates. Antimicrobial resistance was determined by the disk diffusion method, and the presence of genes was detected by polymerase chain reaction. Isolates were typed with Rep-PCR. Results. The results of biofilm formation demonstrated that 85 strains (75.9%) were biofilm producers, and 27 strains (24.1%) were nonproducer isolates. Antibiotic susceptibility pattern in biofilm-positive and biofilm-negative isolates obtained from hospitalized patients showed a high rate of antibiotic resistance to amoxicillin with 95.7% and 92.3%, respectively. Based on PCR amplification results, the frequency of genes involved in biofilm formation among all isolates was as follows: algD (78.6%), pelF (70.5%), pslD (36.6%), Ppgl (0%), and PAPI-1 (77.6%). Rep-PCR typing demonstrated that 112 P. aeruginosa isolates were classified into 57 types according to 70% cut-off. The predominant type was A which contained 15 isolates. Moreover, 7 isolates were clustered in genotype B, followed by C type (6), D (4), E (4), F (4), G (4), H (3), I (3), J (3 isolates), and 12 genotypes, each containing two isolates. Also, 35 isolates were distributed in scattered patterns and showed single types. Conclusion. Study results showed significant association between biofilm formation and resistance to antibiotics such as ceftazidime and meropenem. Analysis of Rep-PCR patterns indicated that the evaluated isolates were heterogeneous, relatively.

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“…The evaluation of capsule production was assessed by culturing the candida strains on Congo red agar (CRA) as described by (23) with minor modifications. The Congo red agar was prepared by mixing 36 g saccharose (Sigma Chemical Company, Lezennes, France) with 0.8 g Congo red in 1 L of on Tryptic Soy Agar (TSA, Difco, City, Spain) supplemented with NaCl (1.0%).…”

Section: Methodsmentioning

confidence: 99%

“…The plates were then inoculated with the Candida spp. and incubated aerobically for 24 h at 37ºC followed by another 24 h at 30 o C. After incubation, black colonies were considered as capsule producers, whereas red colonies were considered as non-producers (23). All tests were done in triplicates that are independent of each other.…”

Section: Determination Of Capsulase Formation Abilitymentioning

confidence: 99%

Incidence, antifungal resistance properties, and virulence traits of candida species isolated from HIV/AIDS Patients from the hospital system in Kenya

Miruka

Eric

Musyoki

et al. 2022

Preprint

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Candidiasis is the most common fungal infection in hospitalized patients with acquired immune deficiency syndrome resulting to morbidity and mortality. This study aimed at characterizing, incidence, susceptibility, resistance genes, antibiofilm activity, and virulence traits of Candida species isolated from HIV-Infected patients. One hundred and eighty-one samples were collected and cultured on Sabouraud Dextrose Agar, biochemical tests and confirmed using automated Vitek-2® Compact bioMérieux followed by susceptibility tests. were done by use of various conventional antifungals against the isolates using standard procedures. Virulence factors, biofilm formations and resistance genes of Candida strains were determined. Out of the 181 samples, 46 were identified as Candida spp., 20 C. albicans (43.5%), 6 C. tropicalis (13.0%), 8 C. krusei (17.4%), 4 C. glabrata (8.7%), 3 C. famata (6.5%), 3 C. parapsilosis (6.5%), and 2 C. guilliermondii (4.3%). All the Candida albicans isolated were both Gram positive and Germ test tube test positive. Eighteen (90%) of the isolates were susceptible to Clotrimazole at a concentration of 5 μg/mL – 10μg/mL followed by 17 (85%) isolates to Panosoconazole at a concentration of 0.002 μg/mL – 5μg/mL. Eight (40.0 %) of the Candida albicans isolates possessed the gene (cdr1) that was observed at 286 bp. Virulence enzymes was determined in which 100% produced Haemolysin, followed by proteinase (75.0%), phospholipase (50%), coagulase at (50%) and lastly capsulase (25.0%). Fluconazole and Clotrimazole did not inhibit growth of C. albicans at high concentrations but from our study, it was deduced that they inhibit biofilm formation at lower concentrations. C. albicans isolates were resistant to multiple antifungal including those commonly used in the management on HIV/AIDs patient. This attributed to resistant genes and produced various virulence factors that were found to be present in the isolates. Therefore, there is a need to carry out regular surveillance on antifungal drug resistance.

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Antimicrobial Susceptibility, Biofilm Production and Adhesion to HEp-2 Cells of <i>Pseudomonas aeruginosa</i> Strains Isolated from Clinical Samples

Cited by 14 publications

References 31 publications

Prevalence of Extended-Spectrum and Metallo β-Lactamase Production in AmpC β-Lactamase Producing Pseudomonas Aeruginosa Isolates From Burns

Prevalence of Extended-Spectrum and Metallo β-Lactamase Production in AmpC β-Lactamase Producing Pseudomonas Aeruginosa Isolates From Burns

Prevalence of algD, pslD, pelF, Ppgl, and PAPI-1 Genes Involved in Biofilm Formation in Clinical Pseudomonas aeruginosa Strains

Incidence, antifungal resistance properties, and virulence traits of candida species isolated from HIV/AIDS Patients from the hospital system in Kenya

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