Antimicrobial Susceptibility and Molecular Epidemiology of Multidrug-Resistant &lt;i&gt;Klebsiella pneumoniae&lt;/i&gt; in Central China

Guo, Xia; Cao, Zaiqiu; Dai, Zhifeng; Li, Yuan; He, Xiaohong; Hu, Xiaoxin; Tian, Fuyun; Ren, Yihui

doi:10.7883/yoken.jjid.2016.049

Cited by 15 publications

(13 citation statements)

References 22 publications

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“…Another study in a hospital in Guangdong reported CTX-M-9 to be the most common CTX-M-type (Du et al, 2014) The most common gene in the current study, bla CTX−M−3 , is rarely reported in China, although this is likely due to a larger volume of data being available on ESBLs among E. coli as compared to K. pneumoniae . For instance, a study on clinical isolates of K. pneumoniae from hospitals in Jilin, China, showed that 48% of CTM-X-producing isolates carried bla CTX−M−3 (Li et al, 2014) and another study on K. pneumoniae isolates collected at a hospital in Henan, China, showed that bla CTX−M−3 was carried by 38% of the CTX-M-producers, which was second in prevalence only by carriers of bla CTX−M−14 (40%) (Guo et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Clinically Relevant Strains of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Occurring in Environmental Sources in a Rural Area of China by Using Whole-Genome Sequencing

et al. 2019

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Klebsiella pneumoniae is a gram-negative, opportunistic pathogen, and a common cause of healthcare-associated infections such as pneumonia, septicemia, and urinary tract infection. The purpose of this study was to survey the occurrence of and characterize K. pneumoniae in different environmental sources in a rural area of Shandong province, China. Two hundred and thirty-one samples from different environmental sources in 12 villages were screened for extended-spectrum β-lactamase-(ESBL)-producing K. pneumoniae , and 14 (6%) samples were positive. All isolates were multidrug-resistant and a few of them belonged to clinically relevant strains which are known to cause hospital outbreaks worldwide. Serotypes, virulence genes, serum survival, and phagocytosis survival were analyzed and the results showed the presence of virulence factors associated with highly virulent clones and a high degree of phagocytosis survivability, indicating the potential virulence of these isolates. These results emphasize the need for further studies designed to elucidate the role of the environment in transmission and dissemination of ESBL-producing K. pneumoniae and the potential risk posed to human and environmental health.

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Section: Discussionmentioning

confidence: 99%

Characterization of Clinically Relevant Strains of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Occurring in Environmental Sources in a Rural Area of China by Using Whole-Genome Sequencing

et al. 2019

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“…However, CTX-M-55 was not identified in clinical practices in China until 2010, when it was detected from a person who traveled to China [ 6 ]. Since then, plenty of surveys have confirmed the emergence of bla CTX - M - 55 among clinical pathogenic in China [ 7 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic contexts related to the diffusion of plasmid-mediated CTX-M-55 extended-spectrum beta-lactamase isolated from Enterobacteriaceae in China

Gou

Guo

et al. 2018

Ann Clin Microbiol Antimicrob

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BackgroundCTX-M-55 extended-spectrum beta-lactamases are being rapidly disseminated and transmitted in clinical practices around the world. The genetic contexts of the transferable plasmid-mediated blaCTX-M-55 gene in Enterobacteriaceae were detected and characterized in this study.MethodsIsolates were obtained from the First Affiliated Hospital of Zhengzhou University between September 2015 and March 2016. Based on polymerase chain reaction and BLAST analysis, resistance genes and genetic context of the blaCTX-M-55 gene were investigated. Conjugation experiments and multilocus sequence typing were performed to demonstrate plasmid-mediated blaCTX-M-55 transmission.ResultsThirteen blaCTX-M-55-positive isolates of Enterobacteriaceae were obtained. Seven isolates were Escherichia coli, 3 were Klebsiella pneumoniae, 1 was Citrobacter freundii, 1 was Morganella morganii and 1 was Serratia marcescens. The blaCTX-M-55 gene has not previously been identified from C. freundii and M. morganii. Four different blaCTX-M-55 genetic contexts were identified, and all of them harbored ISEcp1 in the region upstream of blaCTX-M-55 (in two cases, ISEcp1 was truncated by IS26, and in one case, it was truncated by IS1294), whereas ORF477 was detected downstream of the blaCTX-M-55 gene from 12 of 13 strains. The novel genetic context of ISEcp1∆-blaCTX-M-55-∆IS903 was firstly detected the IS903 element which was identified downstream of blaCTX-M-55. A conjugation assay revealed that all blaCTX-M-55 plasmids were quickly and easily transferable to recipient E. coli, which then presented resistance to multiple antibiotics.ConclusionsNumerous blaCTX-M-55-positive strains were isolated in a short period of 7 months. The findings indicate that blaCTX-M-55 was rapidly disseminated. The genetic context and conjugative transfer found in this study demonstrate that there is active transmission of blaCTX-M-55 among strains of Enterobacteriaceae in China, which could give rise to an urgent global public health threat.

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“…For instance, the KPC-producing K. pneumoniae isolates from central China between 2009 and 2014 were clonally related, with ST11 being the reservoir for the bla KPC-2 and ESBL genes ( bla CTX-M-55 ). These findings demonstrated the high prevalence of carbapenemase- and ESBL-producing K. pneumoniae in central China ( 19 ). An outbreak of KPC-2-producing K. pneumoniae (ST11) in a neonatal ward has been reported ( 20 ).…”

Section: Discussionmentioning

confidence: 70%

Complete Genome Sequence of a bla _KPC-2 -Positive Klebsiella pneumoniae Strain Isolated from the Effluent of an Urban Sewage Treatment Plant in Japan

Sekizuka

Yatsu

Inamine

et al. 2018

mSphere

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We isolated and determined the complete genome sequence of a KPC-2-producing K. pneumoniae strain from a sampling site in Tokyo Bay, Japan, near a wastewater treatment plant (WWTP). In Japan, the KPC type has been very rarely detected, while IMP is the most predominant type of carbapenemase in clinical carbapenemase-producing Enterobacteriaceae (CPE) isolates. Although laboratory testing thus far suggested that Japan may be virtually free of KPC-producing Enterobacteriaceae, we have detected it from effluent from a WWTP. Antimicrobial resistance (AMR) monitoring of WWTP effluent may contribute to the early detection of future AMR bacterial dissemination in clinical settings and communities; indeed, it will help illuminate the whole picture in which environmental contamination through WWTP effluent plays a part.

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Antimicrobial Susceptibility and Molecular Epidemiology of Multidrug-Resistant <i>Klebsiella pneumoniae</i> in Central China

Cited by 15 publications

References 22 publications

Characterization of Clinically Relevant Strains of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Occurring in Environmental Sources in a Rural Area of China by Using Whole-Genome Sequencing

Characterization of Clinically Relevant Strains of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Occurring in Environmental Sources in a Rural Area of China by Using Whole-Genome Sequencing

Genetic contexts related to the diffusion of plasmid-mediated CTX-M-55 extended-spectrum beta-lactamase isolated from Enterobacteriaceae in China

Complete Genome Sequence of a bla _KPC-2 -Positive Klebsiella pneumoniae Strain Isolated from the Effluent of an Urban Sewage Treatment Plant in Japan

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Antimicrobial Susceptibility and Molecular Epidemiology of Multidrug-Resistant <i>Klebsiella pneumoniae</i> in Central China

Cited by 15 publications

References 22 publications

Characterization of Clinically Relevant Strains of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Occurring in Environmental Sources in a Rural Area of China by Using Whole-Genome Sequencing

Characterization of Clinically Relevant Strains of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Occurring in Environmental Sources in a Rural Area of China by Using Whole-Genome Sequencing

Genetic contexts related to the diffusion of plasmid-mediated CTX-M-55 extended-spectrum beta-lactamase isolated from Enterobacteriaceae in China

Complete Genome Sequence of a bla KPC-2 -Positive Klebsiella pneumoniae Strain Isolated from the Effluent of an Urban Sewage Treatment Plant in Japan

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Complete Genome Sequence of a bla _KPC-2 -Positive Klebsiella pneumoniae Strain Isolated from the Effluent of an Urban Sewage Treatment Plant in Japan