Complete Genome Sequence of a bla _KPC-2 -Positive Klebsiella pneumoniae Strain Isolated from the Effluent of an Urban Sewage Treatment Plant in Japan

Abstract

We isolated and determined the complete genome sequence of a KPC-2-producing K. pneumoniae strain from a sampling site in Tokyo Bay, Japan, near a wastewater treatment plant (WWTP). In Japan, the KPC type has been very rarely detected, while IMP is the most predominant type of carbapenemase in clinical carbapenemase-producing Enterobacteriaceae (CPE) isolates. Although laboratory testing thus far suggested that Japan may be virtually free of KPC-producing Enterobacteriaceae, we have detected it from effluent from a WWTP. Antimicrobial resistance (AMR) monitoring of WWTP effluent may contribute to the early detection of future AMR bacterial dissemination in clinical settings and communities; indeed, it will help illuminate the whole picture in which environmental contamination through WWTP effluent plays a part.

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

“…Our study reveals for the first time the presence of carbapenemases-producing K. pneumoniae ST35, ST219, ST364, ST395, ST485 and ST1878 in wastewaters, of which ST395 has clinical importance, while ST35 and ST485 are sporadically related to clinical cases. The other STs, with major clinical significance, have already been described in wastewaters: ST11 [5,41,42,43], ST17 [44], ST45 [45], ST101 [43,46,47,48], ST258 [49], ST307 [45,49,50,51], ST512 [52], and the minor clone ST1564 [45].…”

<p>Molecular Mechanisms and Epidemiology of Carbapenem-Resistant <em>Escherichia coli</em> Isolated from Chinese Patients During 2002–2017</p>

“…The KPC-type enzyme was first reported in Klebsiella pneumoniae from the southern United States in 2001 30 and now endemic all over the world. 31,32 In China, dissemination of KPC-producing Enterobacteriaceae spp. has been confirmed in Shandong, Zhejiang, Taiwan, and other provinces.…”

The Contribution of Wastewater to the Transmission of Antimicrobial Resistance in the Environment: Implications of Mass Gathering Settings

“…These strains have been found to contain ARGs associated with resistance to clinically important drugs, including carbapenems, cephalosporines and aminoglycoside [36]. Additionally, a carbapenemase-producing K. pneumoniae strain carrying bla KPC-2 , which is rarely detected in clinical settings, has been identified in WWTP effluent wastewater in Japan [38].…”