Antimicrobial susceptibility and mechanism of quinolone resistance in Campylobacter jejuni strains isolated from diarrheal patients in a hospital in Tokyo

Bakeli, Gulibahaer; Sato, Kenya; Kumita, Wakako; Saito, Ryoichi; Ono, Emi; Chida, Toshio; Okamura, Noboru

doi:10.1007/s10156-008-0631-2

Cited by 25 publications

(14 citation statements)

References 24 publications

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“…Although the rate of erythromycin resistant C. jejuni isolates has been reported to be 3.2-42% in humans and animals in other countries (Gibreel and Taylor, 2006;Boonmar et al, 2007), none of erythromycin resistant C. jejuni isolates were in this study. This result was consistent with the report in Japan (Bakeli et al, 2008). The prevalence of quinolone-resistant C. jejuni isolates has continued to increase in other countries, although the rate varies from country to country.…”

Section: Discussionsupporting

confidence: 92%

Prevalence of virulence-associated genes and antimicrobial resistance of Campylobacter jejuni from ducks in Gyeongnam Province, Korea

Yang¹,

Kim²,

Lee³

et al. 2014

Korean Journal of Veterinary Service

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Total 99 strains of Campylobacter spp. were isolated from 117 cases of duck's fecal samples. Among 99 strains of Campylobacter spp. isolates, 93 strains (93.9%) were C. jejuni and 6 strains (6.1%) were C. coli. Prevalence of virulence and GBS associated genes of 72 C. jejuni isolates was determined by m-PCR. Among the 10 kinds of virulence associated genes, cadF, dnaJ, flaA and ceuE genes were detected in all of C. jejuni isolates from ducks, racR, pldA, iamA, ciaB, virB11 and docC genes were 87.5%, 84.7%, 77.8%, 48.6%, 13.9% and 11.1%, respectively. Antimicrobial susceptibility test was performed on 72 C. jejuni isolates. The rate of resistance were 62.5% for oxytetracycline, 55.6% for kanamycin, 54.2% for enrofloxacin, 50% for ciprofloxacin, 37.5% for tetracycline and nalidixic acid, 18.1% for ampicillin, 15.3% for streptomycin, and 6.9% for ofloxacin. All isolates were susceptible to erythromycin. The adherence (intracellular and extracellular bacteria) abilities of the 20 isolates to INT-407 cells were between 4.21±1.27×10 4 CFU/well and 1.053±0.451×10 6 CFU/well from the isolates of cj-55 and cj-52, respectively, and that can be expressed as 0.1033% to 5.2655% to the infecting inoculum. The invasion (intracellular bacteria) abilities of the 20 isolates to INT-407 were between 1.00±1.73×10 3 CFU/well and 8.47±5.16×10 4 CFU/well from the isolates of cj-13 and cj-47, respectively, and that can be expressed as 0.0050% to 0.4235% to the infecting inoculums. The average CFU/well of 20 campylobacters isolated from ducks for adherence to and invasion were 2.646±2.886×10 5 and 3.03±2.7×10 4 respectively, and that was 1.3230±1.2139% and 0.1516±0.1343% of the starting viable inoculum. There was considerable correlation (R 2 =0.627) between the adherence and invasion ability of C. jejuni isolates for INT-407 cell.

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Section: Discussionsupporting

confidence: 92%

Prevalence of virulence-associated genes and antimicrobial resistance of Campylobacter jejuni from ducks in Gyeongnam Province, Korea

Yang¹,

Kim²,

Lee³

et al. 2014

Korean Journal of Veterinary Service

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“…Macrolide resistance was not observed in Japan in the current study, which correlates with previous findings showing that C. jejuni isolates were susceptible to clarithromycin and erythromycin (14). However, the resistance rate of C. jejuni (12.5z) observed in this study was greater than that previously reported for C. jejuni isolated from Thai children with diarrhea between 1991 and 2000 (from 0z to 6.3z in 1999) (8,15).…”

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confidence: 81%

“…Fluoroquinolone resistance rates of the Japanese strains were higher than that of a previous study conducted in Japan, which revealed that 37 of 55 (67.3z) C. jejuni isolates tested had the Thr-86-Ile gyrA mutation (14).…”

contrasting

confidence: 65%

Antibiotic Resistance of <i>Campylobacter jejuni</i> and <i>C. coli</i> Isolated from Children with Diarrhea in Thailand and Japan

et al. 2016

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SUMMARY: A total of 29 Campylobacter jejuni and C. coli strains were isolated from Thai and Japanese children with diarrhea using the Loop-mediated Isothermal Amplification method. The samples were evaluated for mutations in gyrA and 23S rRNA in order to assess resistance against fluoroquinolones and macrolides, respectively. Among the isolated strains, 9 (8 C. jejuni and 1 C. coli) were from Thai children, and the other 20 (C. jejuni) were isolated from Japanese children. High fluoroquinolone resistance rates were observed in Thai (66.7z) and Japanese (90z) children. Macrolide resistance was not observed in Japanese children but was observed at a considerable rate of 12.5z of C. jejuni isolated in the Thai cohort. The results indicate that continuous monitoring of resistance of Campylobacter strains to fluoroquinolones and macrolides is definitely necessary.

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“…On the other hand, in case of C. coli strains (n =16), 100% isolates were resistant to nalidixic acid (MIC, 32-128 µg/ml), ciprofloxacin (MIC, 4-128 µg/ml) and sulfamethoxazole-trimethoprim whereas 93.75% isolates were resistant to levofloxacin (MIC, 2-16 µg/ml) and ofloxacin (MIC, 4-32 µg/ml) followed by 62.5% were resistant to ampicillin (MIC, 16-32 µg/ml) and 43.75% were resistant to tetracycline (MIC, 128 µg/ml ) and one C. coli strains had the Thr 86 to Ile and Met 181 to Arg substitution in GyrA and the rest 15 strains had only Thr 86 to Ile substitution in GyrA (Table 6). For Campylobacter strains, high-level resistance to fluoroquinolones is mostly mediated by mutations within the QRDR of the gyrA gene, of which replacement of C256 with T leading to a Thr-86-Ile substitution in GyrA is predominant (Ge et al, 2003;Vacher et al, 2003;Payot et al, 2006;Bakeli et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

Molecular identification and antimicrobial resistance profiles of Campylobacter strains of poultry origin in India with special emphasis on fluoroquinolone resistance

Kabir

Asakura

Shiramaru

et al. 2015

Asian J. Med. Biol. Res

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Abstract:The current study aimed to identify the selected number of Campylobacter strains of poultry origin in India that were isolated in the Laboratory of International Prevention of Epidemics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan to the species level with the aid of cdtA/B/C gene-based multiplex PCR assays as well as to investigate their antimicrobial resistance profiles. C. jejuni (4 strains) and C. coli (16 strains) were identified. The poultry isolates identified were subjected to susceptibility testing with the aid of disk diffusion method using 12 antimicrobial agents. Again, the resistant and intermediate isolates confirmed by the disk diffusion method were subjected to determination of minimum inhibitory concentration by agar dilution method. To elucidate the mechanism of quinolone resistance, a total number of 20 quinolone resistant strains were subjected to sequence determination and analysis of the gyrA gene in the quinolone-resistance determining region. The results of the disk diffusion method were consistent with the results of the agar dilution method with slight variation in case of ampicillin, levofloxacin, ofloxacin and fosfomycin. All C. jejuni and C. coli strains had the Thr 86 to Ile substitution in gyrA. Results of this study support the emergence of resistance of C. jejuni and C. coli strains of poultry origin in India to a variety of antimicrobials especially fluoroquinolones.

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Antimicrobial susceptibility and mechanism of quinolone resistance in Campylobacter jejuni strains isolated from diarrheal patients in a hospital in Tokyo

Cited by 25 publications

References 24 publications

Prevalence of virulence-associated genes and antimicrobial resistance of Campylobacter jejuni from ducks in Gyeongnam Province, Korea

Prevalence of virulence-associated genes and antimicrobial resistance of Campylobacter jejuni from ducks in Gyeongnam Province, Korea

Antibiotic Resistance of <i>Campylobacter jejuni</i> and <i>C. coli</i> Isolated from Children with Diarrhea in Thailand and Japan

Molecular identification and antimicrobial resistance profiles of Campylobacter strains of poultry origin in India with special emphasis on fluoroquinolone resistance

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