Antimicrobial Susceptibilities of Shiga Toxin‐Producing <i>Escherichia coli</i> Isolates from Pigs with Edema Disease in Japan

Uemura, Ryoko; Sueyoshi, Masuo; Nagayoshi, Miki; Nagatomo, Hiroshi

doi:10.1111/j.1348-0421.2003.tb02786.x

Cited by 25 publications

(24 citation statements)

References 8 publications

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“…Inclusion of antibiotics 285 in media has been shown to inhibit certain serotypes (Uemura et al, 2003;Vimont et al, 286 2007a), and strains within serotype. Based on the minimum concentration needed to detect all 287 seven serogroup in spiked feces, EC broth was considered to be the best, which agrees with the 288 results of Vimont et al (2007b).…”

Section: Discussionmentioning

confidence: 99%

Applicability of a multiplex PCR to detect O26, O45, O103, O111, O121, O145, and O157 serogroups of Escherichia coli in cattle feces1

Paddock

Shi

Bai

et al. 2012

Veterinary Microbiology

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Section: Discussionmentioning

confidence: 99%

Applicability of a multiplex PCR to detect O26, O45, O103, O111, O121, O145, and O157 serogroups of Escherichia coli in cattle feces1

Paddock

Shi

Bai

et al. 2012

Veterinary Microbiology

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“…These strains were isolated from pigs with ED in 1999 and 2001, respectively [19]. We also used E. coli ATCC 23546, which was the O139 ETEEC reference strain isolated from pigs with ED in the 1950s [3].…”

Section: Methodsmentioning

confidence: 99%

Effect of Antimicrobial Agents on the Production and Release of Shiga Toxin by Enterotoxaemic <i>Escherichia coli</i> Isolates from Pigs

Uemura

Sueyoshi

Taura

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. Edema disease (ED) of pigs is an enterotoxaemic disease caused by enterotoxaemic Escherichia coli (ETEEC) infection. Antimicrobial therapy for pigs with ED is controversial because it may induce death of sickish piglets. In this study, we investigated the effects in vitro of 7 antimicrobial agents, ampicillin, gentamicin, colistin, bicozamycin, fosfomycin, sulfamethoxazole-trimethoprim and enrofloxacin, on the release and production of shiga toxin (Stx) 2e by ETEEC strains. We found that more Stx 2e accumulated in the bacterial cells than was released into supernatant. Associated with inhibition of cell wall synthesis, the exposure to ampicillin or fosfomycin increased the release of Stx 2e. The production levels of Stx 2e in all antimicrobial-treated cultures were equal to the level in the control or less than in the control. These results suggest that cell wall synthesis inhibitors, such as ampicillin and fosfomycin, may change for the worse in the signs in ETEEC infectious pigs. On the other hand, gentamicin, colistin, bicozamycin and enrofloxac in may be useful for the treatment of pigs with ED. KEY WORDS: antimicrobia, edema disease, enterotoxaemic Escherichia coli, shiga toxin 2e, swine.J. Vet. Med. Sci. 66(8): 899-903, 2004 Edema disease (ED) of the pig has been found throughout the world since ED was first reported in Ireland in 1938 [17]. ED has been a sporadically occurring disease found mainly in post-weaned piglets that causes systemic vascular damage as a result of intestinal infection with shiga toxinproducing Escherichia coli (STEC) [1,2,5]. The STEC is also called enterotoxaemic Escherichia coli (ETEEC) [14]. After ETEEC colonizes in the intestines, shiga toxin (Stx) 2e produced by the ETEEC is absorbed into the capillary vessels and then damages the endothelial cells [1,2,5]. The manifestations of ED include palpebral edema, neurological impairment, lateral recumbence and sudden death [1,2]. The speed of onset and severity of clinical signs and gross pathological lesions are related to the dose of the toxin. Intervals between injection and onset of the disease vary from 7 to 28 hr, the mean time to death being from 24 to 42 hr [2]. Classical ED has been seen to occur sporadically and subside spontaneously [1,2,8,11]. Recently, ED has tended to persist and spread, causing great economic damage to pig farmers [20,21,24].Antimicrobial administration has been used as one of the most common treatments for the eradication of STEC from the intestines of humans and animals [12,14,16,22]. Nevertheless, some antimicrobial agents have been reported to increase the release of Stxs from enterohemorrhagic Escherichia coli (EHEC) associated with enterohemorrhagic colitis and hemolytic uremic syndrome of humans in vitro [4,10,13,22,23]. In ED affected farms, deaths of piglets associated with antimicrobial treatment have increased in number [7,24]. Until now, however, there has been no investigation to determine the effect of antimicrobial agents on the production and release of Stx 2e by ETEEC.In ...

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“…Nowadays, the occurrence of ED has been increasing rapidly in Japan [25,26]. However, the etiology of this disease remains unknown, and its control is difficult [22]. Antimicrobial therapy is used as a major curative treatment for piglets with ED.…”

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confidence: 99%

“…Antimicrobial therapy is used as a major curative treatment for piglets with ED. However, the use of antimicrobials has resulted in the development of antimicrobialresistant STEC on Japanese farms [8,22]. Furthermore, the use of some antimicrobials, such as ampicillin and fosfomycin, is a possible cause for the aggravation of ED symptoms [23].…”

mentioning

confidence: 99%

Experimental Infection of Enterotoxemic Escherichia coli Associated with Porcine Edema Disease and its Pathologic Characteristics in the Intestine

Tsukahara

Nakanishi²,

Nakayama

et al. 2005

J. Vet. Med. Sci.

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ABSTRACT. Edema disease (ED) has become frequent in Japan, but no effective method for experimental infection has been developed. We report here the use of a capsule that resistant against gastric digestion to induce the ED in piglets. Four 21-day-old piglets were used. Shiga toxin 2e-producing Escherichia coli (STEC) cell pellet was encapsulated and administered orally. Two pigs received 1.0 × 10 10 CFU for two days, and the others received 3.9 × 10 8 CFU for three days. The high-dose group caused the typical clinical ED signs (palpebral edema or neurologic impairment). Eosinophil infiltration, swollen lymphoid follicles, and edema were observed in the ileum. The kidney had the thrombus in the glomerulus. KEY WORDS: edema disease, eosinophil infiltration, experimental infection.

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Antimicrobial Susceptibilities of Shiga Toxin‐Producing Escherichia coli Isolates from Pigs with Edema Disease in Japan

Cited by 25 publications

References 8 publications

Applicability of a multiplex PCR to detect O26, O45, O103, O111, O121, O145, and O157 serogroups of Escherichia coli in cattle feces1

Applicability of a multiplex PCR to detect O26, O45, O103, O111, O121, O145, and O157 serogroups of Escherichia coli in cattle feces1

Effect of Antimicrobial Agents on the Production and Release of Shiga Toxin by Enterotoxaemic <i>Escherichia coli</i> Isolates from Pigs

Experimental Infection of Enterotoxemic Escherichia coli Associated with Porcine Edema Disease and its Pathologic Characteristics in the Intestine

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