Antimicrobial susceptibilities and resistance genes in Campylobacter strains isolated from poultry and pigs in Australia

Abstract

Our results suggest the resistant strains are persisting environmental isolates that have been acquired by the different livestock species. Furthermore, the different treatment practices in poultry and pigs have resulted in differences in resistance profiles in Campylobacter isolates.

Resistance to β-lactam and tetracycline in Campylobacter spp.isolated from broiler slaughterhouses in southern Brazil

“…This study found a high level of resistance to β -lactam gene in 65% (91/141) of isolates. Our results are similar to those described by Obeng et al (2012), who observed resistance of 59-65.4% for C. jejuni. In Brazilian studies, Hungaro et al (2015) was found 100% resistant isolates to ampicillin.…”

“…The tetO gene was detected in 35.5% (50/141) of isolates. Previous reports have indicated that tetO is present in 40% of C. jejuni isolates from chicken carcasses (Hungaro et al 2015), and of C. jejuni from chickens 19.2-40.7% (Obeng et al 2012). Tetracyclines have been suggested as an alternative choice in the treatment of clinical campylobacterosis, but in practice are rarely used (Moore et al 2006).…”

“…Primers, PCR conditions and lengths of products generated in this study are listed (Table 1). The PCR conditions were adapted (Pratt & Korolik 2005, Obeng et al 2012). All PCR amplifications were performed in a mixture (25 µL) consisting of 5 µL of 10X PCR Buffer [200 mM Tris-HCl (pH 8.4), 500 mM KCl], 0.25 µL (5U/µL) of Taq thermostable DNA polymerase (Invitrogen ® ), 2 µmol 1 -l of MgCl 2 (25 mM), 2 µL dNTPs (dATP, dCTP, dGTP and dTTP, each at 2.5 mM), 2 µL extracted template DNA and 0.5 µL (10 pmole 1 -l ) of each primer.…”

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

“…In Brazil, the use of this drug has been allowed for therapeutic purposes in poultry production. However, in Norway and Australia, where the use of these antibiotics in aviculture is not allowed, fluoroquinolone resistant Campylobacter were not detected in studies in poultry slaughterhouses (NORSTRÖM et al, 2007;OBENG et al, 2012).…”

Detecção de resistência às fluoroquinolonas em Campylobacter isolados de frangos de criação orgânica

“…Além dos EUA, na Noruega e Austrália, onde também não é permitido o uso de quinolonas na avicultura, estudos realizados relatam a inexistência de cepas de Campylobacter spp. resistentes às fluoroquinolonas (Cui et al 2005, Norström et al 2007, Obeng et al 2012. No Brasil, não é proibido o uso de enrofloxacina, medicamento de uso exclusivo em medicina veterinária, quando este tem fins terapêuticos em avicultura.…”