Antimicrobial Susceptibilities and Phage Typing of Staphylococcus aureus Clinical Isolates in Indonesia

Warsa, Usman Chatib; Okubo, Toyoji; Okamoto, Ryoichi

doi:10.1007/bf02355194

Cited by 11 publications

(4 citation statements)

References 10 publications

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“…Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infection (10), and these bacteria have acquired multiple resistance to a wide range of antibiotics including aminoglycosides (9,10,37). AME produced by MRSA isolates can be determined by identifying the corresponding genes.…”

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confidence: 99%

Identification of Aminoglycoside-Modifying Enzymes by Susceptibility Testing: Epidemiology of Methicillin-Resistant Staphylococcus aureus in Japan

et al. 2001

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A multiple-primer PCR was used to identify genes encoding aminoglycoside-modifying enzymes in 381 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). The technique used three sets of primers delineating specific DNA fragments of the aph(3)-III, ant(4)-I, and aac(6)-aph(2؆) genes, which influence the MICs of gentamicin, tobramycin, and lividomycin. Isolates with none of the three genes detected were susceptible to all three agents. Isolates with the aph(3)-III gene showed resistance to lividomycin (MIC > 1,024 g/ml), and those with the ant(4)-I gene were resistant to tobramycin (MIC > 8 g/ml). Isolates with only the aac(6)-aph(2؆) gene were resistant to gentamicin (MIC > 8 g/ml) and tobramycin in decreasing order; those with both the ant(4)-I and aac(6)-aph(2؆) genes also were resistant to gentamicin and tobramycin, but in increasing order. Susceptibility testing, then, could detect specific genes. In 381 Japanese MRSA isolates, the ant (4)

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confidence: 99%

Identification of Aminoglycoside-Modifying Enzymes by Susceptibility Testing: Epidemiology of Methicillin-Resistant Staphylococcus aureus in Japan

et al. 2001

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“…Kawano et al (1996) verificaram que os SCN meticilina resistente isolados de galinhas saudáveis apresentaram resistência para maioria dos antibióticos â-lactâmicos, e alguns isolados foram também resistentes para os macrolídeos e aminoglicosídeos. A maior causa da resistência aos â-lactâ-micos é atribuída a presença de uma proteína produzida pelo gene mecA (Warsa et al 1996, Hartman & Tomasz, 1984. Apesar da ausência do gene mecA nos isolados de Staphylococcus observou-se neste estudo que os mesmos apresentaram resistência a todos os antimicrobianos.…”

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Perfil de resistência a antimicrobianos de Staphylococcus spp. isolados de frangos de corte e poedeiras comerciais no estado de Pernambuco

et al. 2011

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Este estudo foi realizado com o objetivo de pesquisar Staphylococcus spp. de frangos de corte sadios e frangos de corte e poedeiras comerciais que apresentassem sinais clínicos respiratórios. Foram colhidos swabs dos seios infra-orbitários de 55 frangos de corte sadios, 35 com sinais respiratórios, e 30 poedeiras comerciais também com sinais respiratórios. Cada amostra foi composta por um "pool" de cinco aves, totalizando 24 amostras coletadas de 24 granjas comerciais. Para o isolamento foi utilizado o exame bacteriológico, com posterior avaliação das características morfológicas, tintoriais e bioquímica para determinação da espécie. Verificou-se a produção de hemólise, formação de biofilme em ágar Vermelho Congo (ACR), detecção do gene mecA pela PCR e avaliação da suscetibilidade a 13 drogas antimicrobianas. Das 24 amostras processadas, foram isolados 16 Staphylococcus, cinco isolados foram coagulase-positiva (SCP) e 11 coagulase-negativa (SCN), e nos testes de hemólise e formação de biofilme, três isolados apresentaram-se hemolíticos e seis foram positivos, respectivamente. Na avaliação por meio da PCR, para detecção do gene mecA todos os isolados apresentaram resultados negativo. Observaram-se que 15 isolados foram resistentes a cinco ou mais antibióticos, e que as drogas associadas apresentaram melhor perfil de sensibilidade. A resistência a antimicrobianos e cepas produtoras de biofilme podem interferir na resposta terapêutica de aves que apresentam sinais clínicos.

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“…39,40 Phage group III has been studied to be associated with MRSA. [41][42][43] However, studies did not find significant associations between antibiotic resistance and AD infection. 38,44…”

Section: Bacteriophagesmentioning

confidence: 97%

Updated understanding of Staphylococcus aureus in atopic dermatitis: From virulence factors to commensals and clonal complexes

Hwang

Thompson

Jaros

et al. 2021

Experimental Dermatology

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Atopic dermatitis (AD) is an inflammatory dermatosis with a pathogenesis believed to be due to a combination of genetic, immunologic and environmental factors that affects up to 5% of adults and 20% of children worldwide. 1,2 Common genetic factors include polymorphisms in the filaggrin structural protein, interleukin (IL)-4 receptor and vitamin D receptor. [3][4][5] AD is driven by multiple immune pathways, most commonly with activation of Th2 and Th22 T-cell subsets. 6 Crosstalk between commensals and the host immune system modulates adaptive and innate immune responses in AD. 7 Staphylococcus aureus (SA) in AD lesions and surrounding normal skin was first noted by Leyden et al. in the 1970s; since then, the understanding of its role in AD has drastically evolved. 8,9 SA is a well-established exacerbator of AD; it is frequently isolated from the skin of AD patients and increased SA density is found during flares. 10 A meta-analysis in 2016 reported a pooled prevalence of SA colonization among AD patients of 70% in lesional skin, 39% in non-lesional skin and 62% in the nose. 11 More recently, Kong et al 12 found that AD treatments and flares were closely associated with shifts in the cutaneous microbial diversity. While current evidence has established that SA proliferates during flares and plays a role in the cycle of epidermal breakdown and inflammation, a true causal relationship has not been established. Herein, we will summarize the

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Antimicrobial Susceptibilities and Phage Typing of Staphylococcus aureus Clinical Isolates in Indonesia

Cited by 11 publications

References 10 publications

Identification of Aminoglycoside-Modifying Enzymes by Susceptibility Testing: Epidemiology of Methicillin-Resistant Staphylococcus aureus in Japan

Identification of Aminoglycoside-Modifying Enzymes by Susceptibility Testing: Epidemiology of Methicillin-Resistant Staphylococcus aureus in Japan

Perfil de resistência a antimicrobianos de Staphylococcus spp. isolados de frangos de corte e poedeiras comerciais no estado de Pernambuco

Updated understanding of Staphylococcus aureus in atopic dermatitis: From virulence factors to commensals and clonal complexes

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