Antimicrobial-Resistant
            <i>Campylobacter</i>
            Species from Retail Raw Meats

Ge, Beilei; White, David G.; McDermott, Patrick F.; Girard, W.; Zhao, Shaohua; Hubert, Susannan; Meng, Jianghong

doi:10.1128/aem.69.5.3005-3007.2003

Cited by 130 publications

(108 citation statements)

References 22 publications

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“…On the other hand, in case of C. coli strains (n =16), 100% isolates were resistant to nalidixic acid (MIC, 32-128 µg/ml), ciprofloxacin (MIC, 4-128 µg/ml) and sulfamethoxazole-trimethoprim whereas 93.75% isolates were resistant to levofloxacin (MIC, 2-16 µg/ml) and ofloxacin (MIC, 4-32 µg/ml) followed by 62.5% were resistant to ampicillin (MIC, 16-32 µg/ml) and 43.75% were resistant to tetracycline (MIC, 128 µg/ml ) and one C. coli strains had the Thr 86 to Ile and Met 181 to Arg substitution in GyrA and the rest 15 strains had only Thr 86 to Ile substitution in GyrA (Table 6). For Campylobacter strains, high-level resistance to fluoroquinolones is mostly mediated by mutations within the QRDR of the gyrA gene, of which replacement of C256 with T leading to a Thr-86-Ile substitution in GyrA is predominant (Ge et al, 2003;Vacher et al, 2003;Payot et al, 2006;Bakeli et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

Molecular identification and antimicrobial resistance profiles of Campylobacter strains of poultry origin in India with special emphasis on fluoroquinolone resistance

Kabir

Asakura

Shiramaru

et al. 2015

Asian J. Med. Biol. Res

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Abstract:The current study aimed to identify the selected number of Campylobacter strains of poultry origin in India that were isolated in the Laboratory of International Prevention of Epidemics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan to the species level with the aid of cdtA/B/C gene-based multiplex PCR assays as well as to investigate their antimicrobial resistance profiles. C. jejuni (4 strains) and C. coli (16 strains) were identified. The poultry isolates identified were subjected to susceptibility testing with the aid of disk diffusion method using 12 antimicrobial agents. Again, the resistant and intermediate isolates confirmed by the disk diffusion method were subjected to determination of minimum inhibitory concentration by agar dilution method. To elucidate the mechanism of quinolone resistance, a total number of 20 quinolone resistant strains were subjected to sequence determination and analysis of the gyrA gene in the quinolone-resistance determining region. The results of the disk diffusion method were consistent with the results of the agar dilution method with slight variation in case of ampicillin, levofloxacin, ofloxacin and fosfomycin. All C. jejuni and C. coli strains had the Thr 86 to Ile substitution in gyrA. Results of this study support the emergence of resistance of C. jejuni and C. coli strains of poultry origin in India to a variety of antimicrobials especially fluoroquinolones.

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Section: Resultsmentioning

confidence: 99%

Molecular identification and antimicrobial resistance profiles of Campylobacter strains of poultry origin in India with special emphasis on fluoroquinolone resistance

Kabir

Asakura

Shiramaru

et al. 2015

Asian J. Med. Biol. Res

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“…In the opinion of many authors (Avrain et al 2003, Ge et al 2003, the high resistance to tetracycline is frequently recorded in case of genus Campylobacter bacteria isolated from products of animal origin. This is confirmed by the studies conducted by Sato et al (2004), who recorded 45% of isolates resistant to tetracycline.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance of Campylobacter in raw milk in the selected areas of Poland

Wysok

Wiszniewska-Łaszczych

Uradziński

et al. 2011

Polish Journal of Veterinary Sciences

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During the recent years, an immense increase in the number of food poisoning cases in people caused by Campylobacter (C.) species has occurred. Raw milk, next to poultry meat, is considered the most frequent cause of food poisoning in people caused by the subject bacteria, although it is not always possible to isolate Campylobacter cells from the incriminated milk. Most probably this difficulty is caused by low concentration of the pathogen in milk at the level of 2/3 cells/ml although even such low concentration represents risk to human health. The present study was aimed at determining the occurence of Campylobacter bacteria in milk originating from selected regions of Poland. The isolation method applied in this work was effective in recovering as few as 0.1 cell of Campylobacter per g of food. Among 150 bulk milk samples tested, Campylobacter spp. was isolated from 7 (4.6%) ones. The biochemical identification of the isolated strains conducted by means of conventional biochemical tests as well as by applying the API -Campy tests revealed that all the isolates belonged to the C. jejuni species. Determination of resistance to antibiotics was performed by means of the diffusion disks method for the following antibiotics: gentamicin, ciprofloxacin, ampicillin, chloramphenicol, erythromycin, doxycyclin and tetracycline. Among 7 isolates tested, all were susceptible to ampicillin, chloramphenicol, erythromycin and gentamicin, 28.5% to doxycyclin and 14.2% to tetracycline and ciprofloxacin.

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“…Despite harboring these microorganisms, chickens rarely show signs of clinical disease and act as a source of infection for healthy animals and human beings [5]. The poultry products, especially the retail meat products have been frequently implicated as sources of infection [6][7][8]. Human campylobacteriosis is generally a clinically mild and self-limiting illness, but severe and systemic forms of the disease may require antimicrobial treatment, with macrolides and fluoroquinolones being the drugs of choice [9].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and antibiotic resistance pattern of Campylobacter species in foods of animal origin

Pallavi¹,

Kumar²

2014

Vet World

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Aim:The aim was to determine the prevalence and evaluation of antibiotic resistance pattern and minimum inhibitory concentration (MIC) of Campylobacter species isolated from foods of animal origin. Materials and Methods:A total of 280 samples (comprising 150 chicken meat, 50 chevon and 80 milk) were collected from retail meat markets, slaughter houses and dairy farms and analyzed for isolation of Campylobacter species. A total of 29 isolates comprising 23 Campylobacter jejuni and 6 Campylobacter coli were recovered, characterized biochemically and confirmed by polymerase chain reaction. These isolates were then tested for antibiotic resistance pattern through disc diffusion method, and MIC was assessed by MIC strips. The antibiotic resistance assessment was performed against 8 antibiotics viz. ampicillin, co-trimoxazole, erythromycin, levofloxacin, gentamicin, ciprofloxacin, ceftriaxone, and norfloxacin. Results:The prevalence of Campylobacter spp. in chicken meat, chevon and milk samples were observed 17.33%, 6% and 0%, respectively. All the isolates were resistant to co-trimoxazole but sensitive to erythromycin. All the isolates showed different resistance pattern for the rest of the antibiotics. MIC results revealed that all the isolates were within prescribed concentrations for sensitivity for the antibiotics tested. Conclusions:The foods of animal origin are source of Campylobacter infections to human beings. Thus, the development of antibiotic-resistant strains emphasizes the requirement of better surveillance and monitoring of the foods of animal origin and the use of antimicrobials in veterinary and human medicine require careful regulation.

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Antimicrobial-Resistant Campylobacter Species from Retail Raw Meats

Cited by 130 publications

References 22 publications

Molecular identification and antimicrobial resistance profiles of Campylobacter strains of poultry origin in India with special emphasis on fluoroquinolone resistance

Molecular identification and antimicrobial resistance profiles of Campylobacter strains of poultry origin in India with special emphasis on fluoroquinolone resistance

Prevalence and antimicrobial resistance of Campylobacter in raw milk in the selected areas of Poland

Prevalence and antibiotic resistance pattern of Campylobacter species in foods of animal origin

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