Antimicrobial-resistant Escherichia coli and Enterococcus spp. isolated from Miranda donkey (Equus asinus): an old problem from a new source with a different approach

Carvalho, Isabel; Campo, R. del; Sousa, Margarida; Silva, Nuno; Carrola, João; Marinho, Catarina; Santos, Tiago; Carvalho, Sílvia; Nóvoa, Miguel; Quaresma, Miguel; Pereira, José Eduardo; Cobo, M.; Igrejas, Gilberto; Poeta, Patrícia

doi:10.1099/jmm.0.000423

Cited by 11 publications

(6 citation statements)

References 66 publications

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“…Descriptive analysis comparing the distributions of resistance (R), intermediate (I) and susceptible (S) E. coli , MDR and ESBL-resistant phenotypes as well as β-lactamase genotypes was performed with PROC FREQ (SAS software, version 9.4 SAS Institute, Cary, North Carolina, USA). Isolates were categorized as MDR if they showed resistance to antimicrobial agents in ≥2 different antimicrobial classes [ 49 , 50 ]. To compare the effect of isolate source on AMR in both ESC r and generic E. coli sub-populations, univariate multinomial logistic regression models were fitted to the data using the SAS GLIMMIX procedure [ 13 , 51 ].…”

Section: Methodsmentioning

confidence: 99%

A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant Escherichia coli from Beef Production, Sewage and Clinical Settings

et al. 2020

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This study aimed to compare antimicrobial resistance (AMR) in extended-spectrum cephalosporin-resistant and generic Escherichia coli from a One Health continuum of the beef production system in Alberta, Canada. A total of 705 extended-spectrum cephalosporin-resistant E. coli (ESCr) were obtained from: cattle feces (CFeces, n = 382), catch basins (CBasins, n = 137), surrounding streams (SStreams, n = 59), beef processing plants (BProcessing, n = 4), municipal sewage (MSewage; n = 98) and human clinical specimens (CHumans, n = 25). Generic isolates (663) included: CFeces (n = 142), CBasins (n = 185), SStreams (n = 81), BProcessing (n = 159) and MSewage (n = 96). All isolates were screened for antimicrobial susceptibility to 9 antimicrobials and two clavulanic acid combinations. In ESCr, oxytetracycline (87.7%), ampicillin (84.4%) and streptomycin (73.8%) resistance phenotypes were the most common, with source influencing AMR prevalence (p < 0.001). In generic E. coli, oxytetracycline (51.1%), streptomycin (22.6%), ampicillin (22.5%) and sulfisoxazole (14.3%) resistance were most common. Overall, 88.8% of ESCr, and 26.7% of generic isolates exhibited multi-drug resistance (MDR). MDR in ESCr was high from all sources: CFeces (97.1%), MSewage (96.9%), CHumans (96%), BProcessing (100%), CBasins (70.5%) and SStreams (61.4%). MDR in generic E. coli was lower with CFeces (45.1%), CBasins (34.6%), SStreams (23.5%), MSewage (13.6%) and BProcessing (10.7%). ESBL phenotypes were confirmed in 24.7% (n = 174) ESCr and 0.6% of generic E. coli. Prevalence of bla genes in ESCr were blaCTXM (30.1%), blaCTXM-1 (21.6%), blaTEM (20%), blaCTXM-9 (7.9%), blaOXA (3.0%), blaCTXM-2 (6.4%), blaSHV (1.4%) and AmpC β-lactamase blaCMY (81.3%). The lower AMR in ESCr from SStreams and BProcessing and higher AMR in CHumans and CFeces likely reflects antimicrobial use in these environments. Although MDR levels were higher in ESCr as compared to generic E. coli, AMR to the same antimicrobials ranked high in both ESCr and generic E. coli sub-populations. This suggests that both sub-populations reflect similar AMR trends and are equally useful for AMR surveillance. Considering that MDR ESCr MSewage isolates were obtained without enrichment, while those from CFeces were obtained with enrichment, MSewage may serve as a hot spot for MDR emergence and dissemination.

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Section: Methodsmentioning

confidence: 99%

A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant Escherichia coli from Beef Production, Sewage and Clinical Settings

et al. 2020

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“…Jackson et al [63] report that enterococci is a reservoir of antimicrobial resistance genes and has the potential to transfer these genes to other bacteria in the environment. According to Carvalho et al [64], Escherichia coli and enterococci are reservoirs of antimicrobial-resistant genes that could be disseminated to pathogenic bacteria in the environment. It is essential to place focused effective control measures at decreasing enterococcus in retail meats, since it is a leading cause of nosocomial infections [65].…”

Section: Antimicrobial Drug Resistance In Escherichia Coli and Enteromentioning

confidence: 99%

Prevalence of Multidrug-Resistant Foodborne Pathogens and Indicator Bacteria from Edible Offal and Muscle Meats in Nashville, Tennessee

Liu

Kilonzo‐Nthenge

Nahashon

et al. 2020

Foods

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This study investigated the prevalence of antimicrobial-resistant bacteria in retail edible offal and muscle meats in Nashville, Tennessee. A total of 348 retail meats (160 edible offal and 188 muscle) were analyzed for Salmonella enterica serovar, Campylobacter, Escherichia coli, E. coli O157:H7, and enterococci. Bacteria was identified using biochemical and PCR methods. Salmonella enterica serovar (4.4% and 4.3%), Campylobacter (1.9% and 1.1%), E. coli (79.4% and 89.4%), and enterococci (88.1% and 95.7%) was detected in offal and muscle meats, respectively. Chicken liver (9.7%) was most frequently contaminated with Salmonella enterica serovar, followed by ground chicken (6.9%) and chicken wings (4.2%). No Salmonella enterica serovar was detected in beef liver, beef tripe, and ground beef. The prevalence of Campylobacter was 6.9%, 2.3%, and 1.4% in beef liver, ground beef, and ground chicken, respectively. None of the meats were positive for E. coli O157:H7. Resistance of isolates was significantly (p < 0.05) highest in erythromycin (98.3%; 99.1%), followed by tetracycline (94%; 98.3%), vancomycin (88.8%; 92.2%) as compared to chloramphenicol (43.1%; 53.9%), amoxicillin/clavulanic (43.5%; 45.7%), and ciprofloxacin (45.7%; 55.7%) in offal and muscle meats, respectively. Imipenem showed the lowest resistance (0%; 0.9%). A total of 41 multidrug-resistant patterns were displayed. Edible offal could be a source of antibiotic-resistant bacteria.

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“…coli done in Canarian Egyptian vultures. Even though antibiotic resistance in vultures has been less studied than in other domestic animals worldwide (Ahmed et al ., 2010; Tuerena et al ., 2016; Carvalho et al ., 2017; Saputra et al ., 2017; Rumi et al ., 2019; Suay‐Garcia et al ., 2019), there are some recent available data in different Spanish cities that suggest that Neophron percnopterus might have an important role in the spread of ESBL/pAmpC bacteria (Alcala et al ., 2016; Lopez‐Cerero et al ., 2017; Moreno‐Opo et al ., 2020; Sevilla et al ., 2020). In this way, contact with urban waste and livestock farming has been associated with increased probability of antibiotic‐resistant microbiota in wild birds, especially ESBL‐producing E .…”

Section: Introductionmentioning

confidence: 99%

Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands

Carvalho

Tejedor-Junco

González-Martín

et al. 2020

Environ Microbiol Rep

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Antimicrobial resistance among isolates from wild animals is increasingly reported. Extended‐spectrum beta‐lactamase (ESBL)‐producing Enterobacteriaceae, and particularly Escherichia coli, have spread worldwide as one of the most common multidrug‐resistant organisms. The aim of this study was to determine the carriage rate of ESBL‐producing E. coli isolates and their genetic characteristics in wild vultures from the Canary Islands. Faecal samples were collected from 22 apparently healthy free‐ranging (wild) vulture chicks from Lanzarote and Fuerteventura (Canary Islands) during July 2019. They were seeded in MacConkey agar supplemented with cefotaxime (2 μg ml−1). Colonies with typical morphology of E. coli were identified by MALDI‐TOF‐MS. Antimicrobial susceptibility was done by disk diffusion. Phenotypic detection of ESBL was performed by double‐disk tests. The presence of blaCTX‐M, blaSHV, blaTEM, blaKPC and blaOXA‐48 genes, as well as mcr‐1 (colistin resistance), tetA/tetB and int1 gene, was tested by PCR/sequencing. Phylogenetic groups and multilocus sequence typing (MLST) were determined by PCR/sequencing. ESBL‐producing E. coli isolates were detected in 5/22 tested animals (22.7%), and all isolates (one/animal) carried blaCTX‐M genes: blaCTX‐M‐15 (n = 3) and blaCTX‐M‐55 (n = 2). ESBL‐positive isolates were ascribed to phylogenetic group D (two isolates), B1 (two isolates) and A (one isolate), and five sequence types were detected (ST/phylogenetic‐group/ESBL): ST515/B1/CTX‐M‐15, ST1290/A/CTX‐M‐15, ST38/D/CTX‐M‐15, ST457/D/CTX‐M‐55 and ST6448/B1/CTX‐M‐55; this suggests a genetic diversity among these isolates. Three CTX‐M‐15‐producing isolates contained the blaTEM gene and one the tetA gene. To our knowledge, this appears to be the first report of ESBL‐producing E. coli in vulture chicks from the Canary Islands.

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Antimicrobial-resistant Escherichia coli and Enterococcus spp. isolated from Miranda donkey (Equus asinus): an old problem from a new source with a different approach

Cited by 11 publications

References 66 publications

A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant Escherichia coli from Beef Production, Sewage and Clinical Settings

A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant Escherichia coli from Beef Production, Sewage and Clinical Settings

Prevalence of Multidrug-Resistant Foodborne Pathogens and Indicator Bacteria from Edible Offal and Muscle Meats in Nashville, Tennessee

Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands

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