Antimicrobial resistance, virulence and genetic relationship of Vibrio parahaemolyticus in seafood from coasts of Bohai Sea and Yellow Sea, China

Jiang, Yanhua; Chu, Yubo; Xie, Guosi; Li, Fengling; Wang, Lianzhu; Huang, Jie; Zhai, Yuanzheng; Yao, Lin

doi:10.1016/j.ijfoodmicro.2018.10.005

Cited by 58 publications

(47 citation statements)

References 34 publications

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“…with various virulence factors were identified in inland areas. Interestingly, four STs of V. parahaemolyticus were also reported in a previous study that investigated the genetic diversity of V. parahaemolyticus in Liaoning Province [33]. This finding suggested that the majority of V. parahaemolyticus isolates were likely endemic in this region.…”

Section: Identification Of Virulence Factors In Pathogenic Vibrio Sppsupporting

confidence: 69%

The Impact of Water Intrusion on Pathogenic Vibrio Species to Inland Brackish Waters of China

Wang

Yang

et al. 2020

IJERPH

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The estuary is the ecological niche of pathogenic Vibrio spp. as it provides abundant organic and inorganic nutrients from seawater and rivers. However, little is known about the ecology of these Vibrio species in the inland brackish water area. In this study, their co-occurrence and relationships to key environmental constraints (salinity and temperature) in the Hun-Tai River of China were examined using the most probable number polymerase chain reaction (MPN-PCR) approach. We hereby report 2-year continuous surveillance based on six water indices of the Hun-Tai River. The results showed that seawater intrusion maximally reached inland as far as 26.5 km for the Hun-Tai River. Pathogenic Vibrio spp. were detected in 21.9% of the water samples. In particular, V. cholerae, V. parahaemolyticus, and V. vulnificus were isolated in 10 (10.4%), 20 (20.8.5%), and 2 (2.08%) samples, respectively. All V. parahaemolyticus strains were tdh gene negative, 10% were positive for the trh gene. Multi-locus sequence typing (MLST) divided V. parahaemolyticus strains into 12 sequence types (STs) for the Hun-Tai River. Five STs were respectively present in various locations along the Hun-Tai River. The PCR assay for detecting six virulence genes and Vibrio seventh pandemic island I and II revealed three genotypes in 12 V. cholerae isolates. The results of our study showed that seawater intrusion and salinity have profound effects on the distribution of pathogenic Vibrio spp. in the inland river, suggesting a potential health risk associated with the waters of the Hun-Tai River used for irrigation and drinking.

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Section: Identification Of Virulence Factors In Pathogenic Vibrio Sppsupporting

confidence: 69%

The Impact of Water Intrusion on Pathogenic Vibrio Species to Inland Brackish Waters of China

Wang

Yang

et al. 2020

IJERPH

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“…In our study, high resistance rate of 202 V. parahaemolyticus isolates was observed for some antibiotics, such as ampicillin (79.20%), cephalothin (74.75%), and streptomycin (65.84%). Similarly, the occurrence and outbreak of streptomycin-and ampicillinresistant V. parahaemolyticus isolates were also reported in the past 5 years (Hu and Chen, 2016;Jiang et al, 2019). Besides, antibiotic susceptibility tests displayed the highest level of resistance to ampicillin, signifying that ampicillin may be ineffective and invalid for the treatment of Vibrio sp.…”

Section: Discussionmentioning

confidence: 76%

“…During the past few decades, due to the excessive application of antibiotics in medical treatment and aquaculture industry, antibiotic resistance has emerged and evolved in V. parahaemolyticus (Mazel and Davies, 1999;Cabello, 2006;Elmahdi et al, 2016). Currently, a large number of reports indicate that V. parahaemolyticus isolated from various sources has presented high resistance to single or multiple antibiotics, especially ampicillin (Lee et al, 2018;Fattel et al, 2019;Jiang et al, 2019;Mohamad et al, 2019;Ryu et al, 2019). The frequently occurring phenomenon of multi-drug resistance in V. parahaemolyticus directly affects application of antibiotics and prevention as well as treatment of bacterial infectious diseases (Xu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Food-Borne Vibrio parahaemolyticus in China: Prevalence, Antibiotic Susceptibility, and Genetic Characterization

Xie

Pang

et al. 2020

Front. Microbiol.

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Vibrio parahaemolyticus is a marine and estuarine bacterium that leads to damage of aquatic industry by foodborne outbreaks and possesses an enormous threat to food safety as well as human health worldwide. In the current study, we investigated 905 food samples (ready-to-eat foods, fish, and shrimp) from 15 provinces in China, and aimed to determine prevalence, biological characteristics and genetic diversity of presumptive V. parahaemolyticus isolates. Firstly, 14.17% of 240 fish samples, 15.34% of 365 shrimp samples and 3.67% of 300 RTE food samples were positive for potential V. parahaemolyticus. Secondly, 69 food samples (14.87%) collected in summer were positive for target isolates, while the rate of positive sample of 441 food samples in winter reached 7.26%. Thirdly, we purified 202 V. parahaemolyticus strains for further research. And antimicrobial susceptibility results of strains tested revealed that the highest resistance rate was observed for ampicillin (79.20%). At the same time, 148 (73.27%) of all isolates were classified and defined as multi-drug resistant foodborne bacteria. The results of PCR assay showed that the isolates being positive for the tdh, trh or both genes, were up to 9.90%, 19.80% or 3.96%. Besides, multiplex PCR test showed that the isolates carrying O2 serogroup were the most prevalent. Furthermore, sequence types (STs) of 108 isolates were obtained via multi-locus sequence typing. Not only 82 STs were detected, but also 41 of which were updated in the MLST database. Thus, our findings significantly demonstrated the high contamination rates of V. parahaemolyticus in fish and shrimp and it may possess potential threat for consumer health. We also provided up-to-date dissemination of antibiotic-resistant V. parahaemolyticus which is important to ensure the high efficacy in the treatment of human and aquatic products infections. Lastly, with the identification of 82 STs including 41 novel STs, this study significantly revealed the high genetic diversity among V. parahaemolyticus. All of our research improved our understanding on microbiological risk assessment in ready-to-eat foods, fish, and shrimp.

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“…2016; Jiang et al . 2019b). Although compliance exists in some parts of China where only permitted antibiotics are used in aquaculture sectors, illegal use of banned antibiotics like streptomycin has been reported (Xu et al .…”

Section: Trends Of Antimicrobials In Vibrio Spmentioning

confidence: 99%

“…2017b; Jiang et al . 2019b). This situation may possibly attributable to the illegal use of chloramphenicol or effluent of wastewater in farming areas.…”

Section: Trends Of Antimicrobials In Vibrio Spmentioning

confidence: 99%

Incidence of antibiotic resistance in Vibrio spp

Loo

Letchumanan

Law

et al. 2020

Reviews in Aquaculture

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Vibrio species is a common natural inhabitant of brackish and saltwater. Vibrio sp. includes not only emerging pathogens but also well‐known and emerged pathogens, such as V. cholerae, V. parahaemolyticus and V. vulnificus. Although it is often harmless, pathogenic strains which could cause serious food‐borne illnesses/outbreaks in both humans and animals have been identified. Vibrio infection is usually self‐limiting and requires no clinical treatment. However, antibiotics are still required to treat severe cases of Vibrio infection. Recent reports have highlighted a rise in the presence of antibiotic‐resistant Vibrio in the environment. This hampers the clinical treatment of Vibrio infection and has been linked to the imprudent use of antibiotics in various fields such as human medicine, agriculture and animal husbandry. As a result, it has greatly jeopardized food security, threatened public health and impugned the growth of economy. Food is a potential vehicle of antibiotic‐resistant Vibrio where the resistant genes are transmitted to humans via ingestion of contaminated food. Hence, the surveillance, monitoring and management of antibiotic patterns of Vibrio are important for clinical treatment. This review focuses on different trends and global patterns of antibiotic resistance of Vibrio.

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Antimicrobial resistance, virulence and genetic relationship of Vibrio parahaemolyticus in seafood from coasts of Bohai Sea and Yellow Sea, China

Cited by 58 publications

References 34 publications

The Impact of Water Intrusion on Pathogenic Vibrio Species to Inland Brackish Waters of China

The Impact of Water Intrusion on Pathogenic Vibrio Species to Inland Brackish Waters of China

Food-Borne Vibrio parahaemolyticus in China: Prevalence, Antibiotic Susceptibility, and Genetic Characterization

Incidence of antibiotic resistance in Vibrio spp

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