Antimicrobial Resistance Profile of Salmonella spp. Isolated from Raw Beef Meat Samples Collected from Karachi, Pakistan

Koondhar, Muhammad Noman; Kamboh, Asghar Ali; Khan, M. A.; Leghari, Riaz Ahmed; Dewani, Parkash

doi:10.17582/journal.pjz/20190929130949

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…The Salmonella spp. isolated in the present study were resistant to most of the tested antibiotics and the findings are in line with certain other studies conducted previously in poultry sector and slaughtering facilities of Pakistan [38][39][40].…”

Section: Discussionsupporting

confidence: 93%

An assessment of Hygiene Indicator Bacteria and MDR Salmonella on Poultry Butchers’ hands and Rinsing Water at XDR Salmonella Struck Areas

Tagar

Ahmed

2022

Egyptian Journal of Veterinary Sciences

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F OOD-borne diseases are a major health problem in developing countries including Pakistan. With regard to meat hygiene, the question is posed whether carriers of pathogenic organisms, like Salmonella, shigella, and E. coli can contaminate a product with their hands. This might be possible in case of not using appropriate water, not maintaining proper hand hygiene during mean handling or due to bad toilet hygiene. This study was thus conducted to determine the prevalence of some selected hygiene indicator bacteria on the hands of poultry butchers and their hand washing water in two selected districts of Pakistan, i.e. Hyderabad and Jamshoro. Three hygiene indicator bacteria, Salmonella, Shigella, and E. coli were focused in this regard and for Salmonella spp. antibiotic resistance was also determine. Total 76 samples were collected (38 water and 38 hands). Isolation of bacteria were carried out by some standard microbiological techniques. Out of 38 water samples, 76.3%, 81.5% and 81.5% of samples were positive for Salmonella, Shigella, and E. coli. While for butchers' hand samples, the prevalence of Salmonella, Shigella, and E. coli was found as 92.1%, 97.3%, and 100%. The overall antibiotic resistance is as follows: ampicillin (89%), azithromycin (3.1%), ceftazidime (0%), gentamicin (25.5%), cefotaxime (25%), erythromycin (40.6%), neomycin (31.2%), streptomycin (48.4%), and sulphamethoxazole (50%). Percentage of Multi-drug resistant (MDR) isolates found in water and butchers' hands sample is 50% and 71.4%. One Extremelydrug resistant (XDR) isolate is also found in hands samples. The high prevalence of Salmonella, Shigella, and E. coli in butchers' hands and hand washing water can transfer to the meat and cause many foodborne infections in meat consumers.

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Section: Discussionsupporting

confidence: 93%

An assessment of Hygiene Indicator Bacteria and MDR Salmonella on Poultry Butchers’ hands and Rinsing Water at XDR Salmonella Struck Areas

Tagar

Ahmed

2022

Egyptian Journal of Veterinary Sciences

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“…Foodborne Salmonellosis and acute gastroenteritis caused by Salmonella are major public health issues as a number of such outbreaks have been reported globally. The contamination of Salmonella in poultry meat occurs through the spread of intestinal matter during the cutting of poultry birds (Nair and Kollanoor Johny, 2019).This pathogen has zoonotic potential due to its wide host range (Orji et al, 2005).In developing countries, gastroenteritis causes 1 billion illnesses and 5 million fatalities annually (Koondhar et al, 2021), illustrating the severity of the problem (Heredia and García, 2018).…”

Section: Introductionmentioning

confidence: 99%

Designing a Rapid, Reliable and Reproducible Method for the Detection of Salmonella Spp. From Poultry Meat

Fatima¹,

Nawaz²,

Shahid³

et al. 2022

JMMG

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Salmonella is one of the most common causes of foodborne diseases among humans worldwide. Animal-derived foods are frequently tested for the presence of Salmonella spp. However, the detection of Salmonella in meat and its products is often hindered due to the presence of background normal flora, which may lead to the detection of false-positive Salmonella. The present study aimed to isolate and accurately identify Salmonella spp. from poultry meat. For this purpose, seventy poultry meat samples were collected from Lahore, Pakistan, isolated on selective and differential media, and identified using biochemical tests and polymerase chain reaction for the 16S rRNA gene of identified strains. The results of selective and differential media culturing and biochemical tests were compared with the results of 16S rRNA gene sequencing. It was inferred that the phenylalanine deaminase test and triple sugar iron tests eliminate the false-positive Salmonella isolates obtained on isolation media, and along with the PCR technique, can serve as an accurate and efficient method for the correct detection of Salmonella spp. from meat samples. In order to reduce the false-positive Salmonella isolates, a highly specific selective media must be designed which can distinguish Salmonella forming different colors of colonies from other bacteria and also cause the inhibition of non-Salmonellaisolates.

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Bacteriological quality assessment of poultry chicken meat and meat contact surfaces for the presence of targeted bacteria and determination of antibiotic resistance of Salmonella spp. in Pakistan

Tagar

Qambrani

2023

Food Control

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Antimicrobial Resistance Profile of Salmonella spp. Isolated from Raw Beef Meat Samples Collected from Karachi, Pakistan

Cited by 3 publications

References 4 publications

An assessment of Hygiene Indicator Bacteria and MDR Salmonella on Poultry Butchers’ hands and Rinsing Water at XDR Salmonella Struck Areas

An assessment of Hygiene Indicator Bacteria and MDR Salmonella on Poultry Butchers’ hands and Rinsing Water at XDR Salmonella Struck Areas

Designing a Rapid, Reliable and Reproducible Method for the Detection of Salmonella Spp. From Poultry Meat

Bacteriological quality assessment of poultry chicken meat and meat contact surfaces for the presence of targeted bacteria and determination of antibiotic resistance of Salmonella spp. in Pakistan

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