Antimicrobial Resistance Profile of<i>Salmonella</i>Isolates in Chicken Carcasses in Dakar, Senegal

Fall-Niang, Ndeye Khota; Sambe-Ba, Bissoume; Seck, Abdoulaye; Deme, Saidou Nourou; Wane, Abdoul Aziz; Bercion, Raymond; Alambedji-Bada, Rianatou; Gassama-Sow, Amy

doi:10.1089/fpd.2018.2459

Cited by 21 publications

(16 citation statements)

References 20 publications

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“…Alambedji and colleagues had previously shown that S. Kentucky, S. Muenster and S. Brancaster were the most common in chicken carcasses in Dakar [19]. A recent study conducted in Senegal have also found that the serotypes Istambul, Brancaster and Kentucky were respectively the most prevalent in the broilers [20]. The difference found in the frequency of the serovars by these authors compared to our study could be explained by the fact the they only studied strains of chicken origin.…”

Section: Chicken Broilers 60 35contrasting

confidence: 72%

Antimicrobial Resistant Salmonella Serotypes Circulating in Meat in Senegal

O¹,

KM²,

Ndiaye³

et al. 2021

austinjmicrobiol

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The Presence of antimicrobial-resistant Salmonella in food remains a real threat. The aim of this study was to determine the antimicrobial resistance profiles of Salmonella strains isolated from the most consumed types of meat in Senegal. A total of 337 samples were collected including 247 samples of raw meat and 90 of meat-based ready-made meals. Salmonella strains were isolated according to the French standard EN-ISO-6579 and serotyped according to the KWLM scheme. Antimicrobial susceptibility tests were performed with 16 discs following the agar diffusion method. Our study showed that 37% of the samples carried Salmonella and allowed the isolation of 136 strains of Salmonella. We identified 47 different serotypes including S. Kentucky (11/136: 8%), S. Brancaster (10/136: 7%), S. Chester (10/136: 7%), S. Istanbul (5/136: 4 %), and S. Agona (5/136: 4%) which were respectively the most common. Of the 136 strains, 43 (31.6%) were resistant to at least one antibiotic, of which 15.4% (21/136) were multidrug-resistant. Interestingly, S. Kentucky that was the most common, exhibited also the highest level of resistance, being at least resistant to 8 antibiotics. In conclusion, The increasing resistance and spread of S. Kentucky in meat products demand special monitoring and to be further studied.

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Section: Chicken Broilers 60 35contrasting

confidence: 72%

Antimicrobial Resistant Salmonella Serotypes Circulating in Meat in Senegal

O¹,

KM²,

Ndiaye³

et al. 2021

austinjmicrobiol

View full text Add to dashboard Cite

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“…Moreover, the use of antibiotics in poultry in Côte d’Ivoire should be regulated to reduce the levels of resistance as were observed in the present study. In Côte d’Ivoire [ 36 , 41 ] or in other sub-region countries (Senegal [ 18 , 42 ] and Burkina Faso [ 43 ]), the poultry carcass has been demonstrated to harbor antibiotic-resistant Salmonella coming probably from the primary production and the lack of hygiene in slaughterhouses. Therefore, controlling the antibiotic resistance in primary production will protect consumers and public health against resistant bacteria or resistant genes that can be transmitted to humans through the food chain.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic resistance of Escherichia coli and Salmonella spp. strains isolated from healthy poultry farms in the districts of Abidjan and Agnibilékrou (Côte d'Ivoire)

et al. 2021

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Background and Aim: Antimicrobial resistance (AMR) is a serious challenge to animal and human health worldwide. Therefore, this study aims to determine levels and patterns of AMR of Escherichia coli and Salmonella spp. strains isolated from poultry farms in Côte d'Ivoire. Materials and Methods: A cross-sectional study was conducted in two districts of Côte d'Ivoire with high poultry production: Abidjan and Agnibilékrou. A total of 231 fecal samples were collected in 124 poultry farms in both districts. Enterobacteria were isolated and tested for susceptibility to 14 antimicrobial agents using the disk-diffusion method. Results: A total of 212 E. coli and 36 Salmonella strains were isolated. In Abidjan, 139 collected samples generated 101 E. coli and 23 Salmonella strains, whereas in Agnibilékrou, 92 collected samples generated 111 E. coli and 13 Salmonella strains. Variable resistance levels were recorded for the antibiotics tested. The resistance prevalence of E. coli and Salmonella, respectively, was high: Doxycycline (98%/94%), sulfonamide (84%/86%), trimethoprim-sulfamethoxazole (80%/41%), and streptomycin (71%/52%). Average resistance rates were recorded for flumequine (38%/66%), ampicillin (49%/33%), amoxicillin (25%/44%), colistin (26%/2%), chloramphenicol (21%/2%), and gentamicin (4%/47%). The antibiotics least affected by resistance were cefuroxime (4%/5%), ceftriaxone (2%/0.00%), and nitrofurantoin (1%/0.00%). Conclusion: In this study, it was observed that resistance to important antibiotics is emerging in poultry production in Côte d'Ivoire. Policies promoting the rational use of antibiotics should be implemented to manage antibiotic resistance in animal production.

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“…Considering the pace at which drug resistance is emerging among the pathogens of public health significance, drug discovery, design and their development is the need of the hour [22]. Researchers across the globe have been searching for the novel alternative therapeutic strategies or even drug re-purposing [23,24].…”

Section: Discussionmentioning

confidence: 99%

In silico molecular docking and in vitro antimicrobial efficacy of phytochemicals against multi-drug-resistant enteroaggregative Escherichia coli and non-typhoidal Salmonella spp.

et al. 2021

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Background In the wake of emergence of antimicrobial resistance, bioactive phytochemical compounds are proving to be important therapeutic agents. The present study envisaged in silico molecular docking as well as in vitro antimicrobial efficacy screening of identified phytochemical ligands to the dispersin (aap) and outer membrane osmoporin (OmpC) domains of enteroaggregative Escherichia coli (EAEC) and non-typhoidal Salmonella spp. (NTS), respectively. Materials and methods The evaluation of drug-likeness, molecular properties, and bioactivity of the identified phytocompounds (thymol, carvacrol, and cinnamaldehyde) was carried out using Swiss ADME, while Protox-II and StopTox servers were used to identify its toxicity. The in silico molecular docking of the phytochemical ligands with the protein motifs of dispersin (PDB ID: 2jvu) and outer membrane osmoporin (PDB ID: 3uu2) were carried out using AutoDock v.4.20. Further, the antimicrobial efficacy of these compounds against multi-drug resistant EAEC and NTS strains was determined by estimating the minimum inhibitory concentrations and minimum bactericidal concentrations. Subsequently, these phytochemicals were subjected to their safety (sheep and human erythrocytic haemolysis) as well as stability (cationic salts, and pH) assays. Results All the three identified phytochemicals ligands were found to be zero violators of Lipinski’s rule of five and exhibited drug-likeness. The compounds tested were categorized as toxicity class-4 by Protox-II and were found to be non- cardiotoxic by StopTox. The docking studies employing 3D model of dispersin and ompC motifs with the identified phytochemical ligands exhibited good binding affinity. The identified phytochemical compounds were observed to be comparatively stable at different conditions (cationic salts, and pH); however, a concentration-dependent increase in the haemolytic assay was observed against sheep as well as human erythrocytes. Conclusions In silico molecular docking studies provided useful insights to understand the interaction of phytochemical ligands with protein motifs of pathogen and should be used routinely before the wet screening of any phytochemicals for their antibacterial, stability, and safety aspects.

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Antimicrobial Resistance Profile ofSalmonellaIsolates in Chicken Carcasses in Dakar, Senegal

Cited by 21 publications

References 20 publications

Antimicrobial Resistant Salmonella Serotypes Circulating in Meat in Senegal

Antimicrobial Resistant Salmonella Serotypes Circulating in Meat in Senegal

Antibiotic resistance of Escherichia coli and Salmonella spp. strains isolated from healthy poultry farms in the districts of Abidjan and Agnibilékrou (Côte d'Ivoire)

In silico molecular docking and in vitro antimicrobial efficacy of phytochemicals against multi-drug-resistant enteroaggregative Escherichia coli and non-typhoidal Salmonella spp.

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