Antimicrobial Resistance Pattern and Their Beta-Lactamase Encoding Genes amongPseudomonas aeruginosaStrains Isolated from Cancer Patients

Abstract

This study was designed to investigate the prevalence of metallo-β-lactamases (MBL) and extended-spectrum β-lactamases (ESBL) in P. aeruginosa isolates collected from two different hospitals in Cairo, Egypt. Antibiotic susceptibility testing and phenotypic screening for ESBLs and MBLs were performed on 122 P. aeruginosa isolates collected in the period from January 2011 to March 2012. MICs were determined. ESBLs and MBLs genes were sought by PCR. The resistant rate to imipenem was 39.34%. The resistance rates for P. aeruginosa to cefuroxime, cefoperazone, ceftazidime, aztreonam, and piperacillin/tazobactam were 87.7%, 80.3%, 60.6%, 45.1%, and 25.4%, respectively. Out of 122 P. aeruginosa, 27% and 7.4% were MBL and ESBL, respectively. The prevalence of bla VIM-2, bla OXA-10-, bla VEB-1, bla NDM-, and bla IMP-1-like genes were found in 58.3%, 41.7%, 10.4%, 4.2%, and 2.1%, respectively. GIM-, SPM-, SIM-, and OXA-2-like genes were not detected in this study. OXA-10-like gene was concomitant with VIM-2 and/or VEB. Twelve isolates harbored both OXA-10 and VIM-2; two isolates carried both OXA-10 and VEB. Only one strain contained OXA-10, VIM-2, and VEB. In conclusion, bla VIM-2- and bla OXA-10-like genes were the most prevalent genes in P. aeruginosa in Egypt. To our knowledge, this is the first report of bla VIM-2, bla IMP-1, bla NDM, and bla OXA-10 in P. aeruginosa in Egypt.

Detection of virulence determinants and its association with drug resistance in clinical isolates of Pseudomonas aeruginosa

“…There are several studies showing resistance rate of ceftazidime above 60%, ciprofloxacin from 48-59%, very much higher than our study. [18][19][20][21] Another study by Joseph NM et al, shows that significant reduction in resistant rate of ceftazidime from 50% to 33% and of ciprofloxacin from 49% to 33% much comparable to our study. 22 Several studies showing better anti pseudomonal activity with antibiotics like amikacin and imipenem concurrent to our study.…”

Detection Rate of Metallo-β-Lactamase-Expressing Genes; blaVIM-1, blaVIM-2 and blaSPM-1 in Pseudomonas aeruginosa Isolates

“…16 The most prevalent, introduced families are IMP, VIM, GIM, SPM, and SIM. 1 In our study, 60.7% of the total 60 P. aeruginosa isolates were MBL producer, which were more than the outbreak of MBL producers in Egyptian studies (27% and 32.3%) and Indian study (28.57%). 1 VIM enzymes were also most prevalent in Korea (88%) and Greece (85%).…”

“…1 In our study, 60.7% of the total 60 P. aeruginosa isolates were MBL producer, which were more than the outbreak of MBL producers in Egyptian studies (27% and 32.3%) and Indian study (28.57%). 1 VIM enzymes were also most prevalent in Korea (88%) and Greece (85%). 22 Previous studies demonstrated that IMP and VIM producer genes of MBLs were also common in Asian studies.…”

“…26 In previous studies in Egypt and Taiwan, VIM-2 demonstrated the highest outbreak among imipenem-resistant P. aeruginosa strains. 1,27 Since most of the integrones harbored VIM-1 gene code for aminoglycosides destructing enzymes, the matter is of clinical importance. 28 In the present study, SPM was the commonest (28.5%) gene detected among imipenem-resistant P. aeruginosa isolates.…”

QacE and QacE?1 Genes and Their Correlation to Antibiotics and Biocides Resistance Pseudomonas aeruginosa

“…In recent years, P. aeruginosa emerged in Egypt and it is seen mainly in hospital acquired infections [20,21,22]. The standard definition of MDR is an acquired non-susceptibility to at least one agent in three or more antimicrobial categories [16].…”