Antimicrobial Resistance of Listeria monocytogenes Isolated from Various Cabbage Farms and Packing Sheds in Texas

Prazak, Ann Marie; Murano, Elsa A.; Mercado, I.; Acuff, G. R.

doi:10.4315/0362-028x-65.11.1796

Cited by 49 publications

(19 citation statements)

References 22 publications

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“…Although it was previously considered that multiresistant strains of Listeria spp. are not commonly found in nature, evidence of the emergence of multiresistant L. monocytogenes strains from various sources has been reported (15,16,18). Our results provide further evidence of the emergence of multiresistant strains in nature, representing a potential threat to human health.…”

supporting

confidence: 56%

Occurrence and Antibiotic Sensitivity of Listeria monocytogenes Strains Isolated from Oysters, Fish, and Estuarine Water

Rodas-Suárez

Flores-Pedroche

Betancourt-Rule

et al. 2006

Appl Environ Microbiol

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We analyzed the presence of Listeria spp. in oyster, fish, and seawater samples and tested isolates for antibiotic sensitivity. Listeria monocytogenes was found in 4.5% of fish samples and 8.3% of seawater samples and was not recovered from oysters. Multiresistant environmental strains were found, representing a potential threat to human health.Human listeriosis is a public health problem of low incidence but high mortality, requiring prompt diagnosis and adequate antibiotic therapy (1). Over the last 2 decades a high number of food-borne listeriosis outbreaks have occurred, some with high mortality rates (2,13,19). Antibiotic resistance and inefficient empirical treatment of Listeria infections could be responsible for this increased mortality (4). Since the first multiresistant Listeria monocytogenes strain was observed in France (14), different antibiotic resistance patterns in environmental, food, and clinical sources have been reported (7,12,20). Information on the presence of Listeria monocytogenes in Mexico is scarce, and the frequency of listeriosis is unknown. The purpose of this study was to determine the presence of Listeria spp. in fish, oysters, and saline waters in an area where fish are caught for local and regional consumption and to determine the sensitivities of the L. monocytogenes isolates to different antimicrobial agents.A total of 66 oyster, 66 fish, and 144 estuarine water samples were collected over a 12-month period (June 2001 to May 2002) from 12 sites of the Pueblo Viejo lagoon, Veracruz, Mexico (Fig. 1). Fish and oyster samples were transported on dry ice in separate thermal containers, and estuarine water samples were collected in sterile plastic bottles (Nalgene) and transported to the laboratory on ice. Oyster and fish samples were processed as previously described (9). Seawater samples were filtered through a 14-cm-diameter and 0.45-m-pore membrane (Millipore). Twenty-five milliliters of seawater or 25 g oyster or fish samples was added to 225 ml enrichment broth (EB; Merck) and incubated at 30°C for 24 to 48 h. The filter used for the water samples was washed with 100 ml peptone solution (0.1%), added to 225 ml EB, and incubated at 4°C for 7 days. Afterwards, a 0.1-ml sample was streaked in Oxford agar (Oxoid) and incubated at 30°C for 24 to 48 h. L. monocytogenes isolates were identified and serotyped as previously described (8). Antibiotic sensitivity was assessed using the Kirby-Bauer disk diffusion assay. The test and control strains were seeded in Mueller-Hinton agar supplemented with 0.5% defibrinated sheep blood and 0.1% esculin (17). Commercially available disks (Bio-Rad) with the following antibiotics were used: ampicillin, cephalothin, cefotaxime, ceftazidime, cefuroxime, dicloxacillin, erythromycin, gentamicin, pefloxacin, penicillin, tetracycline, and trimethoprim-sulfamethoxazole. MICs at which 50% of the isolates were inhibited (MIC 50 s) and MIC 90 s were calculated by following the CLSI (formerly NCCLS) guidelines (11). L. monocytogenes ATCC 19114, Escherichia co...

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supporting

confidence: 56%

Occurrence and Antibiotic Sensitivity of Listeria monocytogenes Strains Isolated from Oysters, Fish, and Estuarine Water

Rodas-Suárez

Flores-Pedroche

Betancourt-Rule

et al. 2006

Appl Environ Microbiol

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“…In addition, strains resistant to penicillin (50%) were observed. Prazak and Murano in their study reported the isolation of bacteria susceptible to all antibiotics except penicillin, which corresponds to the results of our study (23). Arslan and Ozdemir (20) in their study reported resistance to penicillin and chloramphenicol, which are in agreement with the results of the present study.…”

Section: Discussionsupporting

confidence: 92%

Phenotypic and Genotypic Characteristics of Listeria monocytogenes Isolated From Dairy and Meat Products

Bahador¹,

Kalani²,

Valian³

et al. 2015

Avicenna J Clin Microbiol Infect

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Background: Listeria monocytogenes is a foodborne pathogen and a serious threat to the public health in the world. Consumption of traditional foods such as dairy and meat products can be a major reason for relative abundance and isolation of these bacteria. Objectives: The purpose of this study was to determine the phenotypic and genotypic characteristics of L. monocytogenes strains isolated from dairy and meat products. Materials and Methods: A total of 317 dairy products and meat-processed samples were collected. Antibiotic susceptibility test was performed on each sample by the disk diffusion method (Kirby Bauer). Five reference loci were used for typing of L. monocytogenes strains by MLVA (Multiple Locus VNTR Analysis) Technique. Results: A total of 24 L. monocytogenes isolates were collected from the dairy and meat products. Resistance of isolated L. monocytogenes strains to penicillin G were 54.54% (from dairy products) and 46.15% (from processed meat). Genetic relatedness of isolates were assessed by MLVA. Out of 13 different types, type 2 with 6 strains and type 3 with 4 strains, were the most common types. Conclusions: MLVA analysis showed that samples obtained from different sources could have similar genetic profile. As a result, administration of penicillin in patients with listeriosis (especially pregnant women) and antibiotic susceptibility test are recommended. The fast and accurate methods such as MLVA for tracking of pollution sources of L. monocytogenes are recommended during outbreaks.

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“…Prazak et al 15 encontraram resultados muito parecidos, onde 95% das cepas de L. monocytogenes oriundas de amostras de repolho, do meio ambiente e de água foram resistentes a dois ou mais antimicrobianos, e 85% destas demonstraram resistência à penicilina. Antunes et al 12 também isolaram cepas de L. monocytogenes, a partir de carcaças de frango, com múltipla resistência antimicrobiana em 74% das amostras analisadas.…”

Section: Resultsunclassified

Resistência antimicrobiana de bactérias do gênero Listeria spp: isoladas de carne moída bovina

Mantilla

Franco

Oliveira

et al. 2008

Braz. J. Vet. Res. Anim. Sci.

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ResumoCom o aumento do uso de antimicrobianos como promotores de crescimento e até mesmo com fins terapêuticos na criação de animais de produção, existe o interesse global referente ao consumo de baixos níveis de resíduos de antimicrobianos em alimentos e os efeitos destes na saúde humana. A ingestão de alimentos contendo resíduos de fármacos antimicrobianos pode ocasionar resistência bacteriana aos antimicrobianos utilizados rotineiramente na terapêutica humana, dificultando o tratamento de enfermidades infecciosas humanas. Neste experimento foi realizado o teste de sensibilidade aos antimicrobianos em cepas de Listeria spp. isoladas a partir de amostras de carnes bovinas, através da metodologia descrita pelo NCCLS em 2003. De acordo com os resultados, todas as cepas isoladas foram resistentes à maioria dos antimicrobianos testados. As cepas de L. monocytogenes foram resistentes à gentamicina, cefoxitina, ampicilina, clindamicina, oxaciclina e sulfazotrim. As cepas de L. innocua isoladas foram resitentes à gentamicina, cefoxitina, tetraciclina, vancomicina, oxaciclina e clindamicina. Palavras-chave:Antimicrobianos. Alimentos. Listeriose. IntroduçãoA utilização de antimicrobianos gerou grande otimismo em relação à prevenção e ao tratamento dos processos infecciosos. Entretanto, o uso exagerado e nem sempre criterioso ou racional dos antimicrobianos e quimioterápicos trouxe dificuldades, sendo a maior delas representada pela progressiva resistência bacteriana aos fármacos. 1 As bactérias podem ser classificadas em sensíveis e resistentes aos antimicrobianos. Em geral, classificam-se como resistentes, as bactérias que crescem "in vitro", nas concentrações médias que os antimicrobianos atingem no sangue, quando administrados por via oral. São sensíveis as que não crescem nestas concentrações. 2 De acordo com Trabulsi e Toledo 2 , a resistência bacteriana pode ser natural ou adquirida. A natural corresponde a uma característica da espécie bacteriana, e a adquirida, à característica de uma ou mais amostras da espécie. A aquisição de resistência por uma célula bacteriana sensível é sempre decorrente de uma alteração genética.O gênero Listeria apresenta uma susceptibilidade uniforme aos antimicrobianos contra bactérias Gram positivas. 3 Os antimicrobianos mais indicados no tratamento da listeriose são : tetraciclina, coumercina, rifampicina, cloranfenicol, eritromicina e ampicilina, sendo esta última adicionada de um antibiótico aminoglicosídio. 4,5 Marth 6 relata que a maioria das cepas é sensível à tetraciclina, cloranfenicol, eritromicina e cefalotina. Ampicilina, rifampicina, ou penicilina associada com gentamicina são os antimicrobianos de escolha para o tratamento de listeriose. 7 A associação de trimetoprim com uma sulfonamida como a sulfametoxazole no co-trimoxazole, é considerada como uma segunda opção para o tratamento. 8 Para o tratamento da bacteremia e da mulher grávida com listeriose, utiliza-se a vancomicina e a eritromicina, respectivamente. 7 Mesmo com o uso destes fármacos, a terapia com antimicrobian...

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Antimicrobial Resistance of Listeria monocytogenes Isolated from Various Cabbage Farms and Packing Sheds in Texas

Cited by 49 publications

References 22 publications

Occurrence and Antibiotic Sensitivity of Listeria monocytogenes Strains Isolated from Oysters, Fish, and Estuarine Water

Occurrence and Antibiotic Sensitivity of Listeria monocytogenes Strains Isolated from Oysters, Fish, and Estuarine Water

Phenotypic and Genotypic Characteristics of Listeria monocytogenes Isolated From Dairy and Meat Products

Resistência antimicrobiana de bactérias do gênero Listeria spp: isoladas de carne moída bovina

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