Antimicrobial Resistance of Klebsiella pneumoniae -ESBL Producing Strains Isolated from Clinical Specimens in Abidjan (Cote de Ivoire)

Abstract

Aims:The objective of this study is to detect the prevalence of qnr genes in broad-spectrum betalactamase producing Klebsiella pneumoniae, involved in hospital and community-acquired infections. Study Design: It is a retrospective study. Place and Duration of Study: Bacteriology and virology laboratorie of Institut Pasteur, Abidjan, Côte d'Ivoire. Methodology: From January 2011 to June 2016, 350 Klebsiella pneumoniae were isolated from various clinical specimens and identified by conventional bacteriological tests. Antibiotic resistance (beta-lactams, quinolones and aminoglycosides) and detection of broad-spectrum beta-lactamases Tahou et al; MRJI, 20(2): 1-7, 2017; Article no.MRJI.34085 2 were assessed by the diffusion method and the synergy test, respectively. Molecular characterization of quinolone resistance genes (qnr A, qnr B and qnr S) was performed by the conventional polymerase chain reaction (PCR). Results: Of the 350 Klebsiella pneumoniae isolates, 91(26%) were detected as ESBL producer, 36.2% (n=33) recovered from urine, 24.2% (n=22) from pus and 20.8% (n=19) from blood culture respectively. 46(33%) strains were idenifed to carry qnr genes, qnrB predominate 33(71%), followed by qnr 12(26.1%) and qnrA 1(2.2%). The strains exhibited high resistance to most of the agents tested, expect imipemen, low resistance to amikacin 4.1%, moderate to cefoxitin 31.8% and 54.9% with amoxicillin-clauvanic acid. Conclusion: Although these observed prevalences are small proportions, this can be seen as a warning signal for the future. The emergence and dissemination of resistance genes in Côte d'Ivoire could pose a public health problem. Thus, the establishment of a relevant resistance surveillance policy to better control the circulation of multidrug-resistant strains is necessary. Original Research Article

Molecular characterization and in silico analysis of mutations associated with extended-spectrum beta-lactamase resistance in uropathogenic Escherichia coli and Klebisiella pneumoniae in two hospitals, Cte dIvoire

“…Commonly found in E. coli, TEM-1 is able to hydrolyze penicillins and narrow spectrum cephalosporins such as cephalothin (Ur Rahman et al, 2018; Galindo- Méndez, 2020). TEM-1 has also been reported in Côte d'Ivoire from previous studies (Guessennd et al 2008;Tahou et al, 2017) in Klebsiella pneumoniae. According to the scientific literature, the TEM-9 variant is detected for the first time in Côte d'Ivoire.…”

“…blaTEM, blaSHV and blaCTX-M are the main variants of genes coding for resistance in Enterobacteriaceae with the latest one (Ahmad and Khadija, 2019). In Côte d'Ivoire, recent studies have reported the spread of ESBL-producing strains of E. coli and K. pneumoniae in various infections (Tahou et al, 2017;Gadou et al, 2018) although the setup since 2002 of an observatory of antimicrobial resistance is dedicated to actions contributing to fight against the spread of resistant or multi-resistant bacteria (Ouédraogo et al, 2017). In addition, information on genetic variants and intragenic mutations encoding these resistances are scarce.…”

Distribution of quinolone resistance gene (qnr) in ESBL-producing Escherichia coli and Klebsiella spp. in Lomé, Togo

“…QnrB was observed to be co-produced with CTX-M-15 in Algeria strains of E. coli [33]. A similar result was recently found in Klebsiella pneumoniae isolates from Côte d’Ivoire, but the type of ESBL was not determined [24]. Qnr genes are usually found in multi-resistance plasmids linked to other resistance determinants, beta-lactamase genes have been conspicuously common [5, 6, 25, 26].…”

“…with 14.6% for qnrB , 9.9% for qnrA and 2.7% for qnrAS [10]. Always in Côte d’Ivoire, others authors found 50.54% of qnr genes in Klebsiella pneumoniae (71.73% qnrB , 26.08% qnrS and 2.17% qnrA ) [24].…”

Assessment of drugs pressure on Escherichia coli and Klebsiella spp. uropathogens in patients attending Abobo-Avocatier Hospital, North of Abidjan (Cte dIvoire)

“…Each urine sample was inoculated on the CHROMAgar medium using plastic loops for 10 μl (Ohkusu, 2000 colonies were colored in metallic blue and medium in size (Ohkusu, 2000). Identification of bacterial species was made based on reactions of Gram, morphology and biochemical characteristics using the gallery of the reduced rack of LEMINOR (Urea; Indol; Simmons' citrate agar; Kligler Hajna agar; iron Lysine agar), oxidase and catalase tests (Tahou et al, 2017). Clean catch urine samples were collected from patients and bacteriological analyses were performed using standard microbiological procedures.…”