Antimicrobial resistance of Aeromonas spp. isolated from the growth pond to the commercial product in a rainbow trout farm following a flumequine treatment

Naviner, Magali; Gordon, Laurence; Giraud, Étienne; Denis, Martine; Mangion, Catherine; Bris, Hervé Le; Ganière, Jean‐Pierre

doi:10.1016/j.aquaculture.2011.03.006

Cited by 36 publications

(29 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Aquatic contamination by these persistent antibiotics resulted in emergence of bacterial resistance and subsequent development of more resistant and virulent pathogens in the exposed environments (Tuševljak et al, 2012). Several studies documented elevated levels of bacterial antibiotic resistance in the surrounding environment of aquaculture production systems, indicating a global health problem (Gordon et al, 2007;Naviner et al, 2011;Sapkota et al, 2008). Emergence of bacterial resistance presents one of the major emerging threats to human health and is by far the highest risk for humans of having medicinal products residues in the environment (EAHC, 2013).…”

Section: As Shown Inmentioning

confidence: 98%

“…Fluoroquinolones (FQ) and tetracyclines (TC), in addition to being employed in human therapy (Cabello, 2006;Heuer et al, 2009;Martinez, 2009;Naviner et al, 2011;Sapkota et al, 2008), are also widely used and effective veterinary antibiotics to prevent and treat fish diseases (FAO, 2010). Thus, FQ and TC were selected as target pharmaceuticals for the present study.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Fluoroquinolones and Tetracycline Antibiotics in a Portuguese Aquaculture System and Aquatic Surroundings: Occurrence and Environmental Impact

Pereira

Silva

Meisel

et al. 2015

Journal of Toxicology and Environmental Health, Part A

View full text Add to dashboard Cite

The growth of aquaculture over the past few years is widely recognized as one of the main sources of antibiotics, mainly fluoroquinolones (FQ) and tetracyclines (TC), in the aquatic environment, consequently, increasing the risk of the emergence of antibiotic bacterial resistance and promoting the spread of resistant genes. This study aimed to (1) develop and validate a multiresidue method for determination and quantification of ciprofloxacin (CIP), difloxacin (DIFL), enrofloxacin (ENR), norfloxacin (NOR), sarafloxacin (SARA), and oxytetracycline (OXY) in aquaculture waters and surrounding water bodies and (2) provide the first Portuguese data to utilize in assessment of risk of adverse effects. In addition, the potential environmental impact posed by these antibiotics to aquatic organisms, belonging to different trophic levels, when exposed to the studied aquaculture waters was also assessed. The analytical strategy comprised of solid-phase extraction (SPE) through Oasis HLB cartridges, and detection and quantification by liquid chromatography with tandem mass spectrometry (LC/MS(n)). Method detection limits (MDL) and method quantification limits (MQL) were in the range of 0.7-3 ng/L and 2.4-10 ng/L, respectively. Recoveries varied between 57.4 and 122.8%. The method was applied to 31 water samples collected from an aquaculture and surrounding water bodies located in north of Portugal. Residues of all antibiotics, except SARA and DIFL, were detected at concentrations ranging from 3 to 75.1 ng/L. Norfloxacin was the antibiotic present at highest frequency and concentration. Regarding the environmental impact assessment (EIA), a risk quotient higher than 1 was observed for NOR.

show abstract

Section: As Shown Inmentioning

confidence: 98%

mentioning

confidence: 99%

Fluoroquinolones and Tetracycline Antibiotics in a Portuguese Aquaculture System and Aquatic Surroundings: Occurrence and Environmental Impact

Pereira

Silva

Meisel

et al. 2015

Journal of Toxicology and Environmental Health, Part A

View full text Add to dashboard Cite

show abstract

“…Moreover, integrons, and other genetic elements are frequently detected in Aeromonas , in respect with these properties, Aeromonas spp. has been studied as an indicator of the dissemination of antimicrobial resistance in water (Usui et al, 2016; Varela et al, 2016) or in fish (Naviner et al, 2006, 2011) excepted for ampicillin, amoxicillin-clavulanate and cefazolin which is an intrinsic resistance for Aeromonas (CLSI, 2015). Monitoring Aeromonas susceptibilities would be much more relevant if standard interpretative criteria, internationally agreed, are applied to the generated data.…”

Section: Introductionmentioning

confidence: 99%

Aeromonas Diversity and Antimicrobial Susceptibility in Freshwater—An Attempt to Set Generic Epidemiological Cut-Off Values

et al. 2017

View full text Add to dashboard Cite

The importance of the role of environment in the dissemination of antimicrobial resistant bacteria is now well recognized. Thus, bacterial indicators to monitor the phenomena are required. The Aeromonas genus is autochthonous in the aquatic environment and easy to detect in any water type, such as freshwater, or wastewater. These microorganisms are also causing infections in humans and animals (including fish). Furthermore, as Aeromonas spp. is able to acquire antimicrobial resistance mechanisms, it is candidate for indicator bacteria to follow antimicrobial resistance dissemination in aquatic environments. Unfortunately, to date, interpretation criteria for Aeromonas spp. for antimicrobial susceptibility tests are scarce in the literature. No epidemiological cut-off values for Aeromonas are currently available at EUCAST to interpret Minimum Inhibitory Concentrations (MIC). The only interpretation criteria available are clinical breakpoints from CLSI that are adapted from Enterobacteriaceae. Based on the results of MIC distributions obtained for a collection of environmental isolates of Aeromonas, this study aimed at proposing tentative epidemiological cut-off values (COWT) for Aeromonas spp. assessing whether the genus is an acceptable level of definition. Thus, 233 isolates collected from 16 rivers were identified at species level using Maldi-Tof (Bruker). Eleven different species were identified, the most abundant were A. bestiarum (n = 54), A. salmonicida (n = 45), A. sobria (n = 41), and A. eucrenophila (n = 37). 96-well micro-plates containing different concentrations of 15 antimicrobials, namely cefotaxime, ceftazidime, chloramphenicol, colistin, enrofloxacin, erythromycin, florfenicol, flumequine, gentamicin, nalidixic acid, oxolinic acid, streptomycin, temocillin, tetracycline, and trimethoprim-sulfamethoxazole, were prepared. The broth micro-dilution method was used to determine the antimicrobial susceptibility of each isolate. The estimation of COWT values was satisfactory obtained at genus level for all antimicrobials except cefotaxime and erythromycin. This first step is an invitation for other research teams to increase the amount of antimicrobial resistance data collected. Then, robustness of our proposed provisional generic epidemiological cut-off values could be assessed by testing antimicrobial susceptibility of various Aeromonas collections.

show abstract

“…Previous reports have indicated that FLU in aquatic ecosystems could trigger specific transcriptional alterations in microbial communities and thus contribute to the development of resistant bacteria (Cabello et al, 2013;Labella et al, 2013). Indeed, antibiotic resistant bacteria and related resistance genes induced by this antibiotic have been widely found in different natural waters (Naviner et al, 2011;Rutgersson et al, 2014). Additionally, FLU can cause certain adverse biological effects on aquatic organisms by inhibiting their growth and reproduction or exhibiting its genotoxic and carcinogenic properties (Zounková et al, 2011;Ma et al, 2012).…”

Section: Introductionmentioning

confidence: 98%

Fast removal of the antibiotic flumequine from aqueous solution by ozonation: Influencing factors, reaction pathways, and toxicity evaluation

Feng

Yan

Zhang

et al. 2016

Science of The Total Environment

View full text Add to dashboard Cite

Antimicrobial resistance of Aeromonas spp. isolated from the growth pond to the commercial product in a rainbow trout farm following a flumequine treatment

Cited by 36 publications

References 34 publications

Fluoroquinolones and Tetracycline Antibiotics in a Portuguese Aquaculture System and Aquatic Surroundings: Occurrence and Environmental Impact

Fluoroquinolones and Tetracycline Antibiotics in a Portuguese Aquaculture System and Aquatic Surroundings: Occurrence and Environmental Impact

Aeromonas Diversity and Antimicrobial Susceptibility in Freshwater—An Attempt to Set Generic Epidemiological Cut-Off Values

Fast removal of the antibiotic flumequine from aqueous solution by ozonation: Influencing factors, reaction pathways, and toxicity evaluation

Contact Info

Product

Resources

About